The concentration-independence cellular effects of fibronectin adsorbed on material surfaces with different hydrophobicities

Huong Le, Hoang-Nghi Mai-Thi, Xuan Le, Ngoc Quyen Tran, Cam Tu Tran, Khon Huynh
Author affiliations

Authors

  • Huong Le School of Biomedical Engineering, International University, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam
  • Hoang-Nghi Mai-Thi School of Biomedical Engineering, International University, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam
  • Xuan Le School of Biomedical Engineering, International University, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam
  • Ngoc Quyen Tran Graduate University of Science and Technology Viet Nam, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Cam Tu Tran Institute of Tropical Biology, Vietnam Academy of Science and Technology, 9/621 Hanoi Highway, Linh Trung Ward, Thu Duc city, Ho Chi Minh city, Vietnam
  • Khon Huynh School of Biomedical Engineering, International University, Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam https://orcid.org/0000-0001-5630-0369

DOI:

https://doi.org/10.15625/1811-4989/16585

Abstract

In tissue engineering, coating biomaterial’s surface with extracellular matrix (ECM) proteins can promote many biological activities, including cellular responses, wound healing, and rejection reduction. Several interactions occur upon binding proteins onto the surfaces, leading to changes in the protein structural conformation, directly affecting the cell-host interactions. Therefore, this study investigates the impacts of surfaces’ wettability on protein conformation. In order to get the insights, organosilicate (OGS) was utilized to modify the tissue culture plate, resulting in surfaces with different wettability, followed by fibronectin (FN) immobilization. Then, the surfaces were used to study the fibrinogen interaction, cell attachment, and spreading. The results showed that OGS-modified surfaces produced four different wettability, ranging from super-hydrophilic (OGS150), hydrophilic (OGS100), hydrophobic (OGS60), to super-hydrophobic (OGS5). Each surface possessed particular nature, resulting in the variation of FN molecules' structural change. The amount of FN adsorbed on the OGS-coated surfaces was shown not to be perfectly proportional to the results of fibrinogen interaction, cell attachment, and spreading. The super-hydrophobic surfaces (OGS5) were highest in the amount of immobilized FN and the efficiency in subsequent experiments among the OGS-coated surfaces group. Notably, the hydrophobic surface adsorbed the lowest amount of FN but achieved remarkable results in the following experiments. Thus, this study holds a promising potential in producing biocompatible materials in tissue engineering.

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Published

30-09-2022

How to Cite

Le, H., Mai-Thi, H.-N., Le, X., Tran, N. Q., Tu Tran, C., & Huynh, K. (2022). The concentration-independence cellular effects of fibronectin adsorbed on material surfaces with different hydrophobicities. Vietnam Journal of Biotechnology, 20(3), 435–444. https://doi.org/10.15625/1811-4989/16585

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Articles