The first record of Metacordyceps neogunnii (Metacordyceps, Clavicipitaceae) isolated from larva of Lepidoptera in Vietnam: morphological, phylogenetic characterization and chemical constituent analysis

Lao Duc Thuan; Le Huyen Ai Thuy; Le Cong Hac; Nguyen Hoang Mai; Nguyen Van Giang; Truong Binh Nguyen

doi:10.15625/1811-4989/16033

Author affiliations

Authors

Lao Duc Thuan Faculty of Biotechnology, Ho Chi Minh City Open University, 97 Vo Van Tan Street, Ward 6, District 3, Ho Chi Minh City, Vietnam
Le Huyen Ai Thuy Faculty of Biotechnology, Ho Chi Minh City Open University, 97 Vo Van Tan Street, Ward 6, District 3, Ho Chi Minh City, Vietnam
Le Cong Hac Faculty of Biotechnology, Ho Chi Minh City Open University, 97 Vo Van Tan Street, Ward 6, District 3, Ho Chi Minh City, Vietnam
Nguyen Hoang Mai Institute of Research & Hi-Tech Application in Agriculture, Dalat University, 1 Phu Dong Thien Vuong Street, Da Lat City, Lamdong Province, Vietnam
Nguyen Van Giang Faculty of Biology, Dalat University, 1 Phu Dong Thien Vuong Street, Da Lat City, Lamdong Province, Vietnam
Truong Binh Nguyen Institute of Research & Hi-Tech Application in Agriculture, Dalat University, 1 Phu Dong Thien Vuong Street, Da Lat City, Lamdong Province, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/16033

Keywords:

Molecular phylogeny, morphological identification, entomopathogenic fungi, adenosine, cordycepin

Abstract

An entomopathogenic fungus, specimen DL0091 parasitized on the larvae of Lepidoptera, was collected from Lang Biang Biosphere Reserve, located in Lam Dong Province, Vietnam. The specimen DL0091 has been analyzed to be contained numerous chemical constituents, especially containing adenosine of 634 mg/Kg and cordycepin of 35.2 mg/Kg. Due to containing many bioactive compounds, DL0091 was promised to be a precious natural source that could be applied in fields of medicine and function food for health care. For classification, based on the morphology analysis, it was identified as Metacordyceps neogunnii (Metacordyceps, Clavicipitaceae) sharing the similar characteristics of M. neogunnii T.C. Wen & K.D. Hyde. Morphology of this species differed from Cordyceps neogunnii (Berk.) Berk., by many characteristics, such as the larger stroma of DL0091 (15–130 mm x 2–6 mm), of asci (550–680 μm × 5–8 μm), etc. Additionally, the combined multi-gene phylogenetic analysis, including ITS, Tef and Rpb1, well supported its systematic position in the clade of M. neogunnii, which was used as traditional herb in China and other Asian countries. In summary, DL0091 was identified as M. neogunnii, containing many bioactive compounds, could be used as the medicinal potential in human healthcare.

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References

Berkeley MJ (1848) Decades of fungi. Decade XX. Tasmanian fungi. London J Bot 7: 572-580.

Chomczynski P (1993) A reagent for the single-step simultaneous Biotechniques 15(3): 536-537.

Jin Y, Meng X, Qiu Z, Su Y, Yu P, Qu P (2018) Anti-tumor and antimetastatic roles of cordycepin, one bioactive compound of Cordyceps militaris. Saudi J Biol Sci 25(5): 991-995.

Kobayashi Y (1941) The genus Cordyceps and its allies. Sci Rep Tokyo Bunrika Daigaku Sect B 5: 53-260.

Kobayashi Y (1982) Keys to the taxa of the genera Cordyceps and Torrubiella. Trans Mycol Soc Japan 23: 329-364.

Kuo HC, Huang IC, Chen TY (2015) Cordyceps s.L. (ascomycetes) species used as medicinal mushrooms are closely related with higher ability to produce cordycepin. Int J Med Mushrooms 17(11): 1077-1085.

Li Y, Chen W, Han Y, Liang J, Liang Z (2020) Cordyceps yinjiangensis, a new ant-pathogenic fungus. Phytotaxa 453(3): 284-292.

Li Z, Li C, Huang B, Fan M, Lee M (1999) new variety of cordyceps gunnii (Berk.) Berk. and its paecilomyces anamorph. Korean J Mycol 27(3): 231-233.

Liu Y, Wang J, Wang W, Zhang H, Zhang X, Han C (2015) The chemical constituents and pharmacological actions of Cordyceps sinensis. J Evid Compl Alter Med 1-12.

Meng M, Wang H, Li Z, Guo M, Hou L (2019) Protective effects of polysaccharides from Cordyceps gunnii mycelia against cyclophosphamide-induced immunosuppression to TLR4/TRAF6/NF-κB signalling in BALB/c mice. Food Funct 10(6): 3262-3271.

She X, Yao T, Ding L, Gan Z, Tang M, Han Y (2019) Identification and analysis of secondary metabolites from CH3OH/H2O extract of Metacordyceps neogunnii. E3S Web Conf, 118.

Shin S, Moon S, Park Y, Kwon J, Lee S, Lee CK, Cho K, Ha NJ, Kim K (2009) Role of cordycepin and adenosine on the phenotypic switch of macrophages via induced anti-inflammatory cytokines. Immune Netw 9(6): 255.

Sung GH, Hywel-Jones NL, Sung JM, Luangsaard JJ, Shrestha B, Spatafora JW (2007) Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol 57: 5-59.

Wen TC, Xiao YP, Han YF, Huang SK, Zha LS, Hyde KD, Kang JC (2017) Multigene phylogeny and morphology reveal that the Chinese medicinal mushroom ‘Cordyceps gunnii’ is Metacordyceps neogunnii sp. Nov. Phytotaxa 302(1): 27-39.

Yang FQ, Feng K, Zhao J, Li SP (2009) Analysis of sterols and fatty acids in natural and cultured Cordyceps by one-step derivatization followed with gas chromatography–mass spectrometry. J Pharmaceut Biomed 49(5): 1172-1178.

Zhu ZY, Liu XC, Tang YL, Dong FY, Sun HQ, Chen L, Zhang, YM (2016) Effects of cultural medium on the formation and antitumor activity of polysaccharides by Cordyceps gunnii. J Biosci Bioeng 122(4): 494-498.

Zhu ZY, Liu Y, Si CL, Yuan J, Lv Q, Li YY, Dong GL, Liu AJ, Zhang YM (2013) Sulfated modification of the polysaccharide from Cordyceps gunnii mycelia and its biological activities. Carbohydr Polym 92(1): 872-876.