Molecular evolutionary relationships of Vietnamese and global pulmonary Paragonimus species in the family Paragonimidae and suborder Xiphidiata (Platyhelminthes: Trematoda)

Nguyen Thi Khue, Pham Thi Khanh Linh, Do Thi Roan, Doan Thi Thanh Huong, Pham Ngoc Doanh, Le Thanh Hoa
Author affiliations

Authors

  • Nguyen Thi Khue 1Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST); 2Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST)
  • Pham Thi Khanh Linh 1Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST)
  • Do Thi Roan 1Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST);
  • Doan Thi Thanh Huong 1Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST); 2Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST)
  • Pham Ngoc Doanh 3Institute of Ecology and Biological Resources (IEBR), Vietnam Academy of Science and Technology (VAST)
  • Le Thanh Hoa 1Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST); 2Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST) https://orcid.org/0000-0003-3841-368X

DOI:

https://doi.org/10.15625/1811-4989/18/4/15673

Keywords:

28S rDNA/rRNA, ribosomal transcription unit, Paragonimus spp., Paragonimus westermani, phylogenetic, complex, evolution, Vietnam

Abstract

Paragonimiasis, caused by Paragonimus species belonging to the family Paragonimidae of the suborder Xiphidiata (Platyhelminthes: Trematoda), often occurs in poor, upland, ethnic minorities, in Vietnam and the world. Asian Paragonimus species are distributed from Japan, South Korea, along with North and Southeast China, North-West and Central Vietnam, the Philippines, Thailand, Bangladesh, India, and Sri Lanka. There are various genetic variants, strains, and genotypes forming different complexes and evolutionary lineages. The 18S, 28S rDNA sequences and the intergenic transcribed spacer regions (ITS-1, ITS-2) of nuclear ribosomal transcription units are commonly used as molecular markers in genetic studies and phylogenetic analyses. We obtained a portion of 28S rDNA (domains D1–D3) of Paragonimus spp. including P. heterotremus (from Vietnam), P. ohirai (Japan), P. iloktsuenensis (Japan), and P. westermani (India and Vietnam) and conducted phylogenetic analysis for molecular evolutionary studies. The results showed that the family Paragonimidae formed the biggest cluster in a phylogenetic tree, which comprises of 46 sequences of 11 species belonging to 11 subgroups, among which the P. westermani complex of strains originating from China, Korea, Japan, India, Philippines, Malaysia, and Vietnam is present. P. westermani complex is arranged in a position of "sister" (sister group) with the subgroup P. siamensis. The P. heterotremus and P. ohirai complexes, and the P. miyazakii, P. harinasutai, P. mexicanus, P. kellicotti, and P. macrorchis complexes are clustered in a common population. P. westermani of Vietnam is in close proximity to the East Asian strains, as of which has been previously reported. P. ohirai and P. iloktsuenensis are considered “sibling” species, sharing the same clade. Phylogenetic analysis using the 28S rDNA sequences directly presented species position and their molecular evolutionary relationships in the families Paragonimidae, Troglotrematidae, Nanophyetidae, and Collyriclidae. Evolutionary analysis has also clarified a number of complex delineation problems and made a clear nomenclature for Paragonimus sp. of Vietnam, in particular, which has scientific grounds merited to recognize as that it is really the P. westermani species.

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References

Blair D (2006) Ribosomal DNA variation in parasitic flatworms. In: Maule A, editor. Parasitic Flatworms: Molecular Biology, Biochemistry, Immunology and Control. CAB International pp. 96–123.

Blair D (2008) Family Paragonimidae Dollfus, 1939. In: Bray RA, Gibson DI, Jones A, eds. Keys to the Trematoda. Vol. 3. London: CAB International and Natural History Museum.

Blair D (2014) Paragonimasis. In: Toledo R, Fried B (editors). Digenetic Trematodes. New York: Springer Science, pp. 115–52.

Blair D, Nawa Y, Mitreva M, Doanh PN (2016) Gene diversity and genetic variation in lung flukes (genus Paragonimus). Trans R Soc Trop Med Hyg 110(1): 6–12.

Cumberlidge N, Rollinson D, Vercruysse J, Tchuem Tchuenté LA, Webster B, Clark PF (2018) Paragonimus and paragonimiasis in West and Central Africa: unresolved questions. Parasitology 145(13): 1748–1757.

Doanh PN, Horii Y, Nawa Y (2013) Paragonimus and paragonimiasis in Vietnam: an update. Korean J Parasitol 51(6): 621–627.

Doanh PN, Shinohara A, Horii Y, Habe S, Nawa Y (2009) Discovery of Paragonimus westermani in Vietnam and its molecular phylogenetic status in P. westermani complex. Parasitol Res 104(5): 1149–1155.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K ( 2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol 35: 1547–1549.

Le TH, De NV, Blair D, McManus DP, Kino H and Agatsuma T (2006) Paragonimus heterotremus Chen and Hsia, 1964, in Vietnam: a molecular identification and relationships of isolates from different hosts and geographical origins. Acta Trop 98(1): 25–33.

Le TH, Nguyen KT, Nguyen NT, Doan HT, Dung DT, Blair D (2017) The ribosomal transcription units of Haplorchis pumilio and H. taichui and the use of 28S sequences for phylogenetic identification of common heterophyids in Vietnam. Parasites & Vectors 10: 17.

Le TH, Nguyen KT, Nguyen NTB, Doan HTT, Agatsuma T, Blair D (2019) The complete mitochondrial genome of Paragonimus ohirai (Paragonimidae: Trematoda: Platyhelminthes) and its comparison with P. westermani congeners and other trematodes. PeerJ 7: e7031.

Le TH, Pham KLT, Doan HTT, Le TKX, Nguyen KT, Lawton SP (2020) Description and phylogenetic analyses of ribosomal transcription units from species of Fasciolidae (Platyhelminthes: Digenea). Journal of Helminthology 94: e136.

Le Thanh Hoa, Nguyen Thi Bich Nga, Doan Thi Thanh Huong, Le Thi Thi Kim Xuyen, Nguyen Thi Khue (2019) Structure and characterization of the ribosomal transcription units of small liver flukes, Opisthorchis viverrini, O. felineus and Clonorchis sinensis. Journal of Biotechnology 17(3): 561–567 (in Vietnamese with English abstract).

McStay B (2016). Nucleolar organizer regions: genomic 'dark matter' requiring illumination. Genes & Development 30(14): 1598–1610.

Pérez-Ponce de León G, Hernández-Mena DI (2019) Testing the higherlevel phylogenetic classification of Digenea (Platyhelminthes, Trematoda) based on nuclear rDNA sequences before entering the age of the ‘next-generation’ Tree of Life. Journal of Helminthology 93: 260–276.

Procop GW (2009) North American paragonimiasis (caused by Paragonimus kellicotti) in the context of global paragonimiasis. Clin Microbiol Rev 22(3): 415–446.

Sanpool O, Intapan PM, Thanchomnang T, Janwan P, Laymanivong S, Sugiyama H, Maleewong W (2015) Morphological and molecular identification of a lung fluke, Paragonimus macrorchis (Trematoda, Paragonimidae), found in central Lao PDR and its molecular phylogenetic status in the genus Paragonimus. Parasitol Int 64(6): 513–518.

Sanpool O, Intapan PM, Thanchomnang T, Janwan P, Nawa Y, Blair D, Maleewong W (2013) Molecular variation in the Paragonimus heterotremus complex in Thailand and Myanmar. Korean J Parasitol 51(6): 677–681.

Tkach V, Pawlowski J, Mariaux J (2000). Phylogenetic analysis of the suborder plagiorchiata (Platyhelminthes, Digenea) based on partial lsrDNA sequences. Int J Parasitol 30(1): 83–93.

Weider LJ, Elser JJ, Crease TJ, Mateos M, Cotner JB, Markow TA (2005). The functional significance of ribosomal rDNA variation: Impacts on the evolutionary ecology of organisms. Annu Rev Ecol Evol Syst 36: 219–242.

Yoshida A, Doanh PN, Maruyama H (2019). Paragonimus and paragonimiasis in Asia: An update. Acta Trop 199: 105074.

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Published

24-05-2021

How to Cite

Khue, N. T., Linh, P. T. K., Roan, D. T., Huong, D. T. T., Doanh, P. N., & Hoa, L. T. (2021). Molecular evolutionary relationships of Vietnamese and global pulmonary Paragonimus species in the family Paragonimidae and suborder Xiphidiata (Platyhelminthes: Trematoda). Vietnam Journal of Biotechnology, 18(4), 653–662. https://doi.org/10.15625/1811-4989/18/4/15673

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