Direct induction of adventitious root in Schefflera octophylla (Lour.) Harms from leaf explants cultured in vitro
Author affiliations
DOI:
https://doi.org/10.15625/1811-4989/15621Keywords:
Chất điều hòa sinh trưởng thực vật, mô lá, môi trường nuôi cấy, Schefflera octophylla, sự hình thành trực tiếp rễ bất địnhAbstract
Schefflera octophylla (Lour.) Harms is a precious plant species belonging to the Araliaceae family. All parts of plant have been used to create products for human health. In tissue culture of medicinal plants, the induction and multiplication of adventitious root of Schefflera octophylla for biomass collection have been studied. In this report, results on induction of adventitious root from leaf explants cultured in vitro of this plant species were presented. Leaf disks (~ 10 x 10 mm), leaf transverse - thin cell layers (t-TCLs) (~ 3 x 10 mm) were cultured on different mineral media MS, ½MS, B5, SH with NAA (0 - 5 mg/L), sucrose (0 - 50 g/L) and light intensity (0 - 4,000 lux). The results showed that, 30 days after culturing on ½MS solid medium plus 3 mg/L NAA, and 30 g/L sucrose in 4,000 lux light condition, direct formation of adventitious root was best from leaf disks, t-TCLs with rooting rate (%) 100, 100; root number/sample 68.80, 21.96; root lenght (mm) 16.53, 15.53, respectively. Leaf disk culture resulted in better rooting than t-TCL culture in two criteria of root number and root length. Morphological and histological observations of adventitious root primordia formation in the leaf disk were also performed. This is the first report on direct formation of adventitious root by in vitro culture of leaf disks/t-TCLs in Schefflera octophylla with very high efficiency, creating basis for further studies on root biomass multiplication for production of bioactive compounds.
Downloads
References
Chen YF, Tao SH, Zeng FL, Xie LW, Shen ZB (2015) Antinociceptive and anti-inflammatory activities of Schefflera octophylla extracts. J Ethnopharmacol 171: 42–50.
Đặng Thị Thanh Tâm (2012) Nghiên cứu tạo rễ tơ của cây Tam thất, Ngũ gia bì chân chim và thử nghiệm quá trình sản xuất sinh khối bằng bioreactor. Báo cáo tổng hợp đề tài KC.04.TN10/11-15 thuộc Chương trình KH&CN trọng điểm cấp nhà nước (KC.04/11-15), Trường ĐH. Nông nghiệp Hà Nội, 151 tr.
Đỗ Huy Bích, Đặng Quang Chung, Bùi Xuân Chương, Nguyễn Thượng Dong, Đỗ Trung Đàm, Phạm Văn Hiền, Vũ Ngọc Lộ, Phạm Duy Mai, Phạm Kim Mãn, Đoàn Thị Thu, Nguyễn Tập, Trần Toàn (2006) Cây thuốc và động vật làm thuốc ở Việt Nam, NXB Khoa học và Kỹ thuật, Viện Dược Liệu, 2: 411-414.
Dương Tấn Nhựt (2010) Một số phương pháp, hệ thống mới trong nghiên cứu công nghệ sinh học thực vật. NXB Nông nghiệp TP. HCM, 218 tr.
Eduardo SV (1998) In vitro root induction and plumule explants of Helianthus annuus. Environmental and Experimental Botany 39: 271-277.
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirement of suspensions cultures of soybean root cells. Exp Cell Res 50(1):151-158.
Hussein S, Ling APK, Ng TH, Ibrahim R and Paek KY (2012) Adventitious roots induction of recalcitrant tropical woody plant, Eurycoma longifolia. Romanian Biotechnological Letters 17(1): 7026-7025.
Huỳnh Thị Lũy, Nguyễn Hữu Hổ, Bùi Văn Lệ (2019) Tạo phôi vô tính trực tiếp từ nuôi cấy in vitro từ mô lá cây Ngũ gia bì chân chim Schefflera octophylla (Lour.) Harms. Kỷ yếu Hội nghị Công nghệ Sinh học toàn quốc, TP. HCM, 01/11/2019, tr. 405-410 (ISBN 978 – 604 – 73 – 7266 - 9).
Imin N, Nizamidin M, Daniher D, Nolan KE, Rose RJ and Rolfe BG (2005) Proteomic analysis of somatic embryogenesis in Medicago truncatula. Plant Physiology 137: 1250-1260.
James DJ (1983) Adventitious root formation in vitro in apple rookstock (Malus pumila). Phyosilogia Plantarum 57: 213 -218.
Khalafalla MM, Daffalla HM, El-Shemy HA and Abdellatef E (2009) Establishment of in vitro fast-growing normal root culture of Vernonia amygdalina - a potent African medicinal plant. African Journal of Biotechnology 8(21): 5952-5957.
Kim HJ, Chang EJ, Oh HI (2005) Saponin production in submerged adventitious root culture of Panax ginseng as affected by culture conditions and elicitors. Asia Pacific Journal Molecular Biology Biotechnology 13: 87-91.
Kitajima J, Shindo M, Tanaka Y (1990) Two new triterpenoid sulfates from the leaves of Schefflera octophylla. Chem.Pharm. Bull. 38: 714–716.
Lã Đình Mỡi, Châu Văn Minh, Trần Văn Sung, Phạm Quốc Long, Phan Văn Kiệm, Trần Huy Thái, Trần Minh Hợi, Ninh Khắc Bản, Lê Mai Hương (2013) Họ Nhân sâm (Araliaceae Juss.) - Nguồn hoạt chất sinh học đa dạng và đầy triển vọng ở Việt Nam. Tuyển tập Hội nghị khoa học toàn quốc lần thứ 5 về Sinh thái và tài nguyên sinh vật (Viện HLKH&CNVN, 18/10/2013), tr. 1152-1158.
Li Y, Shi DX, Wang ML (2004) Tissue culture and rapid propagation of Schefflera octophylla (Abstract). Plant Physiology Communications 40(2): 193. Science Press.
Li YL, Jiang RW, Ooi LSM, But PPH, Ooi VEC (2007) Antiviral triterpenoids from the medicinal plant Schefflera heptaphylla. Phytother Res 21: 466–470.
Li YL, Yeung CM, Chiu LCM, Cen YZ and Ooi VEC (2009) Chemical composition and antiproliferative activity of essential oil from the leaves of a medicinal herb, Schefflera heptaphylla. Phytother Res 23: 140–142.
Ling APK, Chin MF, Hussein S (2009a) Adventitious roots production of Centella asiatica in response to plant regulators and sucrose concentration. Medicinal and Aromatic Plant Science and Biotechnology 3(1): 36-42.
Ling APK, Kok KM, Hussein S and Ong SL (2009b) Effects of plant growth regulators on adventitious roots induction from different explants of Orthosiphon stamineus. American-Eurasian Journal of Sustainable Agriculture 3(3): 493-501.
Ling APK, Tan KP, Hussein S (2013) Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. Alata. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) 14(7): 621-631.
Liu X, Dong J, Liang Q, Wang HMD, Liu Z, Xu R and Kang W (2019b) Coagulant effects and mechanism of Schefflera heptaphylla (L.) Frodin. Molecules 24(24), 4547; https://doi.org/10.3390/molecules24244547.
Liu X, Niu Y, Liu J, Shi M, Xu R and Kang W (2019a) Efficient extraction of anti-inflammatory active ingredients from Schefflera octophylla leaves using ionic liquid-based ultrasonic-assisted extraction coupled with HPLC. Molecules 24(16), 2942; https://doi.org/10.3390/molecules24162942.
Lo KH (1997) Factor affecting shoot organogenesis in leaf dic culture of African violet. Scientia Hoticulturae 72(1): 49-57.
Mahdieh M, Noori M and Hoseinkhani S (2015) Establishment of in vitro adventitious root cultures and analysis of flavonoids in Rumex crispus. Plant Tissue Cult & Biotech 25(1): 63-70.
Martin KP, Zhang CL, Hembrom ME, Slater A, Madassery J (2008) Adventitious root induction in Ophiorrhiza prostrata: a tool for the production of camptothecin (an anticancer drug) and rapid propagation. Plant Biotechnol Rep 2: 163–169.
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15: 473-497.
Nandagopal S and Kumari BDR (2007) Effectiveness of auxin induced in vitro root culture in chicory. J Cent Eur Agric 8(1): 73-80.
Nguyễn Thị Liễu, Nguyễn Trung Thành, Nguyễn Văn Kết (2011) Nghiên cứu khả năng tạo rễ bất định của sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.) trong nuôi cấy in vitro. Tạp chí Khoa học ĐHQGHN, Khoa học Tự nhiên và Công nghệ 27: 30-36.
Nguyễn Thị Ngọc Hương, Trần Hùng, Trương Thị Đẹp (2016) Tìm hiểu các biến đổi hình thái trong sự phát sinh rễ Tam thất hoang (Panax stipuleanatus H.T.Tsai et K.M.Feng) nuôi cấy in vitro và bước đầu định tính oleanolic acid trong rễ tạo thành. Tạp chí Công nghệ Sinh học 14(1): 49-54.
Nguyễn Thị Ngọc Hương, Trần Hùng, Trương Thị Đẹp (2018) Nghiên cứu sự hình thành rễ từ cuống lá cây Tam thất hoang (Panax stipuleanatus H.T.Tsai et K.M.Feng) in vitro và bước đầu định lượng saponin toàn phần trong rễ tạo thành. Tạp chí Y học TP. HCM 22(1): 45-51.
Pandey VP, Cherian E and Patani G (2010) Effect of growth regulators and culture conditions on direct root induction of Rauwolfia serpentina L. (Apocynaceae) Benth by leaf explants. Tropical Journal of Pharmaceutical Research 9(1): 27-34.
Rahmat E and Kang Y (2019) Adventitious root culture for secondary metabolite production in medicinal plants: A Review. J Plant Biotechnol 46: 143–157.
Roberts K (2007) Auxin. In: Handbook of Plant Science. John Wiley & Sons Ltd., USA, pp. 352-360.
Rose RJ, Wang XD, Nolan KE and Rolfe BG (2006) Root meristems in Medicago truncatula tissue culture arise from vascular-derived procambial-like cells in a process regulated by ethylene. Journal of Experimental Botany 57(10): 2227–2235.
Saiman MZ, Mustafa NR, Schulte AE, Verpoorte R, Choi YH (2012) Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens. Plant Cell Tiss Organ Cult 109: 465–475.
Samaj J, Bobak M, Kubosnikova D, Kristin J, Kolarik E, Ovecka M, Blehova A (1999) Bundle sheet cells are responsible for direct root regeneration from leaf explants of Helianthus occidentalis. J Plant Physiol 154: 89-94.
Schenk RU and Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell culture. Can J Bot 50: 199-204.
Schmidt EDL, Guzzo F, Toonen MAJ and Devries SC (1997) A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development 124: 2049-2062.
Sharmaa SN, Jhaa Z and Sinha RK (2013) Establishment of in vitro adventitious root cultures and analysis of andrographolide in Andrographis paniculata. Natural Product Communications 8(8): 1045-1047.
Silva ER, Simoes IM, Baptista JO, Schmildt ER, Lopes JC, Goncalves EO, Caldeira MVW, Alexandre RS (2020) In vitro of Melanoxylon brauna Schott. morphogenesis: responsiveness of explants to permanent and temporary immersion growth regulators. CERNE 26(1): 26-36.
Sumaryono, Wirdhatul M, Diah R (2012) Effect of carbohydrate source on growth and performance of in vitro sago palm (Metroxylon sagu Rottb.) plantlets. HAYATI Journal of Biosciences 19(2): 88-92.
Taj F, Khan MA, Ali H and Khan RS (2019) Improved production of industrially important essential oils through elicitation in the adventitious roots of Artemisia amygdalina. Plants 8, 430; doi:10.3390/plants8100430.
Tchouga AO and Deblauwe V (2020) Micropropagation and effect of
phloroglucinol on rooting of Diospyros crassiflora Hiern. Hortscience 55(4): 424–428
Thomas C, Meyer D, Himber C and Steinmetz A (2004) Spatial expression of a sunflower SERK gene during induction of somatic embryogenesis and shoot organogenesis. Plant Physiology and Biochemistry 42: 35-42.
Yu KW, Gao WY, Hahn EJ, and Paek KY (2001) Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in ginseng (Panax ginseng C.A. Meyer). Journal of Plant Biology 44(4): 179-184.
Zhang JY, Sun HJ, Song IJ, Bae TW, Kang HG, Ko SM, Kwon YI, Kim IW, Lee J, Park SY, Lim PO, Kim YH, Lee HY (2014) Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation 60Co of adventitious roots. J. Ginseng Res 38: 220-225.
Zhou S and Brown DCW (2006) High efficiency plant production of North American ginseng via somatic embryogenesis from cotyledon explants. Plant Cell Rep 25: 166–173.
Downloads
Published
How to Cite
Issue
Section
