Assessment of gene and protein similarities of Beijing-1 vaccine producing strain with Japanese encephalitis virus strains circulating in Vietnam

Authors

  • Nguyen Thi Ly Institute of Biotechnology, VAST
  • Huynh Thi Phuong Lien
  • Do Tuan Dat
  • Nguyen Kim Bach
  • Nguyen Hoang Tung
  • Ha Thi Thu
  • Dinh Duy Khang

DOI:

https://doi.org/10.15625/1811-4989/18/4/15398

Keywords:

Beijing-1 strain, Master Seed Virus strain, Working Seed Virus strain, Envelope protein gene, nucleotide homology, amino acid similarity, neutralizing antibody-produce epitopes.

Abstract

Since 2006, the inactivated Japanese encephalitis vaccine has been studied and produced by the VABIOTECH company from the Beịing-1 strain on Vero cells. Vaccines produced from the materseed and working seed virus have been evaluated at laboratory and clinical scale in humans. The results showed that the vaccine was safe and created 100% protective antibodies after the booster dose. To officially put this vaccine into production and mass use, the master seed virus BV-MSV-0210 and working seed virus BV-WSV-0310 with the reference standard strain JEV Beijing-Kanonji has been tested for genetic stability. By the method of Sanger sequencing and genetic analysis software, we have evaluated the similarity of nucleotide and proteinsequencesof the E antigen-encoding gene. The results showed that the seed virus similarity of amino acids and nucleotides is 100% compared with the reference strain. Thus, it can be concluded, the seed virus has antigen stability. Nucleotide and amino acid gene sequences of E genomic regions of the two seed lots were compared with virus strains isolated from human, pig and mosquito in Vietnam. The results showed that the nucleotide similarity of seed virus compared with the JEV strains isolated from humans ranged from 86.67 to 97.54%; from pigs is 87.47 to 88.33%, and from mosquitoes is 86.05 to 99%. Meanwhile, the amino acid similarity of the seed virus with the JEV strains isolated from humans ranged from 96.73 to 99.02%; from pigs is 98.00 to98.40% and from mosquitoes 94.55 to 98.40%. The sequence of amino acids in the epitope producing neutralizing antibodies of the seed virus did not differ from that of the JEV strain circulating in humans isolated in 2014.

Downloads

Download data is not yet available.

Author Biography

Nguyen Thi Ly, Institute of Biotechnology, VAST

Molecular Microbiology Laboratory

References

Đỗ Tuấn Đạt, Vũ Đình Thiểm, Tống Thiện Anh, Nguyễn Thị Lý (2017) Tính an toàn và sinh miễn dịch của vắc xin Viêm não nhật bản (VNNB) sản xuất trên tế bào Vero (JECEVAX) ở trẻ em (giai đoạn II)”. Tạp chí y học dự phòng, 27:

Dược điểm Châu Âu, 2017.

Huỳnh Phương Liên, Nguyễn Anh Tuấn, Trần Hằng Nga, Nguyễn Quế Anh và cs (2011) Nghiên cứu phát triển vắc xin VNNB bất hoạt trên tế bào vero. Đề tài cấp bộ.

Huỳnh Phương Liên, Nguyễn Anh Tuấn, Trần Hằng Nga, Nguyễn Quế Anh và cs (2011) Đánh giá tính an toàn và sinh miễn dịch của vắc xin VNNB bất hoạt trên tế bào vero. Đề tài cấp nhà nước.

Luca VC, AbiMansour J, Nelson CA, and Fremont DH (2012) Crystal Structure of the Japanese Encephalitis Virus Envelope Protein. J Virol 86: 2337–2346.

Nguyễn Thị Lý, Huỳnh Phương Liên, Đỗ Tuấn Đạt, Đoàn Hữu Thiển, Phạm Văn Hùng, Nguyễn Thị Mai Hương (2018) Chất lượng vắc xin viêm não nhật bản bất hoạt sản xuất trên tế bào Vero tại VIệt Nam. Hội nghị KHCN Sinh học toàn quốc 2018, 26/10/2018. NXB Khoa học tự nhiên và công nghệ, 2018, trang 512.

Roy U (2020) Structural and molecular analyses of functional epitopes and escape mutants in Japanese encephalitis virus envelope protein domain III. Immunol Res 22: 1-9.

Sharma P, Mittal V, Chhabra M, Singh P, Chauhan LS, Rai Arvind (2014) An Update on JE Vaccine Development and Use. J Commun Dis 46: 109- 118.

WHO TRS No. 963, (2007) Annex 1. Recommendations for Japanese encephalitis vaccine (inactivated) for human use.

Downloads

Published

2021-05-24

Issue

Section

Articles