Isolation and screening producing growth regulator cyanobacteria strains
Keywords:Cyanobacteria, indole-3-acetic acid (IAA), isolate, phytohormone, Planktothricoides raciborskii.
AbstractCyanobacteria are photosynthetic microorganisms that have their biosynthesis capacities for secondary compounds with the high application value. They can produce a variety of bioactive compounds such as lipopeptides, fatty acids, toxins, carotenoids, vitamins and plant growth regulators which could be released into the culture medium. The present study aimed to isolate and screen cyanobacteria strains that could synthesize phytohormone, indole-3-acetic acid (IAA) from paddy soild and fresh water ecosystems (canals, river). Soil and water samples were collected from diferent provinces (Bac Giang, Thanh Hoa and Hue). Indole-3-acetic acid was extracted from the culture of isolated cyanobacteria strains and identified using the Salkowski method. As a result, total 10 strains belonging to 4 genera including Nostoc, Anabena, Geitlerinema and Planktothricoides were susscessful isolated from river, canal and rice field. The morphology of isolated taxa was characterized and monoalgal cultures were grown in BG 11 medium. In L-tryptophan-enriched growth media, all cyanobacteria strains in this research were able to biosynthesize growth regulators with IAA concentrations ranging from 9.1 to 95 µg/mL. Among the isolated cyanobacteria strains, the Planktothricoides raciborskii showed potential for the production of IAA even in the absence of tryptophan in the culture medium. Research results of the L-tryptophan concentration effect on the ability of IAA biosynthesis of this cyanobacteria strain showed that IAA concentration increased gradually and reached the highest value (118,28 ± 2,00 µg/mL) when supplementing L- tryptophan in culture medium at 900 µg/mL. The capacity of producing IAA makes these isolated cyanobacteria an appopriate cadidate for agricultural biotechnology.
Ahmad F, Ahmad I and Khan MS (2005). Indole acetic acid production by the indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and in the absence of tryptophan. Turk J Biol, 29:29-34.
Ahmed M, Stal LJ and Hasnain H (2010). Production of indole- 3-acetic acid by the cyanobacterium Arthrospira platensis Strain MMG-9. J. Microbiol. Biotechnol, 20(9):1259-1265.
Amarsinh B, Pravin P and Sunil P (2016). Screening and optimization of indole 3 acetic acid producing non-heterocystous cyanobacteria isolated from saline soil. Scholars Academic Journal of Biosciences, 4(9): 738-744.
Ashok KB, Perumal V and Sivakumar N (2013). Indole-3-acetic acid from filamentous cyanobacteria: Screening, strain identification and production. Journal of Scienttific & Industrial Research, Vol. 72, pp. 581-584.
Chittapun S, Limbipichai S, Amnuaysin N, Boonkerd R, Charoensook M (2018). Effects of using cyanobacteria and fertilizer on growth and yield of rice, Pathum Thani I: a pot experiment. J Appl Phycol 30:79–85
Duong TT (1996) Taxonomy of Cyanobacteria of Vietnam. Agriculture Publishing House, Hanoi
Hazarika D, Duarah I and Barukial J (2012). An ecological assessment of algal growth with particular reference to blue-green algae from upper Brahmaputra valley of Assam, Indian Journal of fundamental and applied life science, 2(3), 29–35.
Gollerbakh MM and Shtina AE, Tảo đất, NXB Leningrat, 1969, 228 tr.
Komárek J, Anagnostidis K (1999). Cyanoprokaryota, 1. Teil, Chroococcales. - In: Ettl, H., Gärtner, G., Heynig, H., Mollenhauer, D. (eds): Süsswasserflora von Mitteleuropa 19/1 (pp 1-548). Fischer Ver lag, Jena.
Mazhar S, Hasnain S (2011) Screening of native plant growth promoting cyanobacteria and their impact on Triticum aestivum var. Uqab 2000 growth. Afr J Agric Res 6 (17): 3988-3993
Mehboob A, Stal LJ, Hasnain S (2010) Production of indole-3-acetic acid Arthrospira platensis platensis strain MMG-9. J Microbiol Biotechn 20:1259-1265
Prasanna R, Jaiswal P, Kaushik BD (2008). Cyanobacteria as potential options for environmental sustainability- promises and challenges. Indian J. Microbiol, 48:89-94
Prasanna R, Jishi M, Rana A, Nain L (2010) Modulation of IAA production in cyanobacteria by tryptophan and light. Pol J Microbio 59: 99-105
Shirai M, Matumaru K, Ohotake A, Takamura Y, Tokujiro A, Nakano M (1989). Development of a Solid Medium for Growth and Isolation of Axenic Microcystis Strains (Cyanobacteria). Applied an environmental Microbiology, 55: 2569-2571.
Singh DP, Prabha R, Yandigeri MS, Arora DK (2011). Cyanobacteria-mediated phenylpropanoids and phytohormones in rice (Oryza sativa) enhance plant growth and stress tolerance. Antonie van Leeuwenhoek, 100:557–568.
Rahman A, Sitepu IR, Tang SY and Hashidoko Y (2010). Salkowski’s Reagent Test as a Primary Screening Index for Functionalities of Rhizobacteria Isolated from Wild Dipterocarp Saplings Growing Naturally on Medium-Strongly Acidic Tropical Peat Soil. Biosci. Biotechnol. Biochem., 74 (11): 2202–2208.
Whitton B.A., Potts M (2000). The Ecology of Cyanobacteria: Their Diversity in Time and Space, Kluwer Academic Publisher, Dordrecht, The Netherlands, pp. 669.