Synthesis of graphene oxide-TiO2 nanotubes-silver nanoparticles nanocomposite by gamma irradiation for antibacterial and post-harvest preservation purposes


  • Nguyen Thi Phuong Anh
  • Pham Thi Thuy Loan
  • Nguyen Vu Duy Khang
  • Vo Nguyen Dang Khoa



Graphene oxide, TiO2 nanotubes, silver nanoparticles, Escherichia coli, nanocomposite, post-harvest crops


In this study, graphene oxide (GO)-TiO2 nanotubes (TNTs)-silver nanoparticles (AgNPs) nanocomposites were synthesized under γ-ray irradiation at different doses (5, 10, 15, 20 and 25 kGy) from formerly synthesized GO, TNTs and AgNPs. They were then characterized by Fourier-transformed infrared (FTIR) and ultra-violet-visible (UV-Vis) spectroscopies, as well as by scanning electron (SEM) and transmission electron (TEM) microscopes. The spectral data indicated the assemblage of silver nanoparticles on both GO sheets and TiO2 nanotubes, as well as the assemblage of TiO2 nanotubes on GO sheets. In addition, their antibacterial activity against Escherichia coli and post-harvest preservation were investigated. Fresh bunches of green grapes were used for this study. AATCC 100-2012 and ISO 21527-1:2008 standards were used for all experiments. The obtained results indicated that all nanocomposite samples exhibited very high antibacterial activity against E. coli. Among which, the 20 kGy sample showed the highest value. Moreover, two samples (5 kGy and 25 kGy) possessed the lower number of yeasts and molds than that of control sample, indicating that the nanocomposites had partial contribution to the preservation of post-harvest crops. We have also found in this study that the dose range affected the antibacterial activity and preservation; and the highest dose range, however, was not always ideal for that purpose. With such fascinating properties, GO-TNTs-AgNPs will be the promising material for antibacterial and agricultural applications.


Aherne D, Ledwith DM, Gara M, Kelly JM (2008) Optical Properties and Growth Aspects of Silver Nanoprisms Produced by a Highly Reproducible and Rapid Synthesis at Room Temperature. Advanced Functional Materials 18: 2005-2016

Bykkam S, Rao VK, Chakra CHS, and Thumgunta T (2013) Synthesis and Characterization of Graphene Oxide and Its Antimicrobial Activity Agents Klebseilla and Staphylococcus. Int. J. Adv. Biotech. & Res. 1(4): 142-146

Cui Y, Kundalwal S, Kumar S (2016) Gas barrier performance of graphene/polymer nanocomposites. Carbon 98: 313-333

Gupta DK, Rajaura RS, Jasuja ND, Sharma K (2015) Synthesis and characterization of graphene oxide nanoparticles and their antibacterial activity. Journal of Environment, Science and Technology 1(1): 16-24

Hummers WS, Offeman RE (1958) Preparation of graphitic oxide. Journal of the American Chemical Society 1339-1339

Kunrath MF, Leal BF, Hubler R, de Oliveira SD, Teixeira ER (2019) Antibacterial potential associated with drug-delivery built TiO2 nanotubes in biomedical implants. AMB Expr. 9(51): 1-13

Li Y, Yang Y, Li R, Tang X, Guo D, Qing Y, Qin Y (2019) Enhanced antibacterial properties of orthopedic implants by titanium nanotube surface modification: a review of current techniques. International Journal of Nanomedicine 14: 7217-7236

Marcano DC, Kosynkin DV, Berlin JM, Sinitskii A, Sun Z, Slesarev A, Alemany LB, Lu W, Tour JM (2010) Improved Synthesis of Graphene Oxide. ACS Nano 4(8): 4806-4814

Selim A, Elhaig MM, Taha SA, Nasr EA (2018) Antibacterial activity of silver nanoparticles against field and reference strains of Mycobacterium tuberculosis, Mycobacterium bovis and multiple-drug-resistant tuberculosis strains. Rev. Sci. Tech. Off. Int. Epiz. 37(3): 1-16

Smith AT, LaChance AM, Zeng S, Liu B, Sun L (2019) Synthesis, properties, and applications of graphene oxide/reduced graphene oxide and their nanocomposites. Nano Materials Science 1(1): 31-47

Zavala MAL, Ávila-Santos M (2017) Synthesis of stable TiO2 nanotubes: effect of hydrothermal treatment, acid washing and annealing temperature. Heliyon 3(2017): 1-18