. Identification of CYP2C9, VKORC1 genotypes and recommendation of warfarin dose for Vietnamese cardiovascular patients

Nguyen Dang Ton; Nguyen Thi Thanh Hoa; Nguyen Phan Anh; Vu Phuong Nhung; Le Thi Bich Thao; Nguyen Hai Ha

doi:10.15625/1811-4989/17/4/14568

Author affiliations

Authors

Nguyen Dang Ton
Nguyen Thi Thanh Hoa
Nguyen Phan Anh
Vu Phuong Nhung
Le Thi Bich Thao
Nguyen Hai Ha Institute of genome research

DOI:

https://doi.org/10.15625/1811-4989/17/4/14568

Keywords:

Warfarin, pharmacogenetics, CYP2C9 gene, VKORC1 gene, PCR-RFLP

Abstract

Warfarin is a well-known anticoagulant that capable of reducing the activity of vitamin K-dependent clotting factors. It has been widely used for cardiovascular patients. However, patient’s genotype of CYP2C9 and VKORC1 remarkably affects the metabolism of warfarin. This study aims to identify the CYP2C9 and VKORC1 genotypes of 96 Vietnamese patients suffering cardiodynia or myocardial infarction and establish their daily warfarin dose. The PCR-RFLP method was used to identified the CYP2C9*2, CYP2C9*3 and VKORC1 alleles. The result indicated that allelic frequencies for CYP2C9*3 were observed at 3% of investigated patients while CYP2C9*2 alleles and genotypes were not detected in this study population. Allele frequency of VKORC1 (c. -1639G>A) was observed at 84%. Base on the CYP2C9 and VKORC1 genotypes, we recommended the daily warfarin dose for of these patients.

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