Physiological and molecular comparative analysis of two contrasting rice varieties under jasmonic acid treatment
Keywords:Rice (Oryza sativa L.), Jasmonic acid, abiotic stress, phosphorus starvation, JA–responsive genes
Rice is one of the most important crops but its productivity is severely threatened by both biotic and abiotic stresses. Jasmonic acid and its derivatives (referred to JA) are the lipid-based plant hormones that were commonly known as regulators of plant growth, development and defense responses. In this study, we compare the physiological and molecular responses of two rice accessions, G38 (Nep_nuong) and G11 (Tam-tron) to JA treatment. G38 plants under JA treatment experienced a reduction in shoot length, root weight, shoot weight and total plant weight which suggested its sensitiveness to JA, whereas G11 plants showed a less reduction in these traits. The expression levels of 12 JA-related genes were investigated in order to better understand how the JA biosynthesis and responses differ in these two contrasting rice accessions. A significantly higher expression level of a set of genes related to JA biosynthesis, signaling and response in G11 compare to G38 was observed. Furthermore, the inorganic phosphorus starvation (Pi) response was also examined in the two varieties G11 and G38. In low Pi condition (40 µM), G11 plants showed more roots, longer root length and shoot length, higher weight compared to the G38 plants which suggest that G11 did not suffer much effect of Pi deficiency. This study highlights the differences in JA growth response in 2 contrasting rice genotypes and also suggests the link between JA developmental response and the tolerance to the Pi starvation condition in rice.
Bates, T.R., Lynch, J.P., 2000. The efficiency of Arabidopsis thaliana (Brassicaceae) root hairs in phosphorus acquisition. Am. J. Bot. 87, 964–970.
Chini, A., Boter, M., Solano, R., 2009. Plant oxylipins: COI1/JAZs/MYC2 as the core jasmonic acid-signalling module. FEBS J. 276, 4682–4692. https://doi.org/10.1111/j.1742-4658.2009.07194.x
Chini, A., Fonseca, S., Fernández, G., Adie, B., Chico, J.M., Lorenzo, O., García-Casado, G., López-Vidriero, I., Lozano, F.M., Ponce, M.R., Micol, J.L., Solano, R., 2007. The JAZ family of repressors is the missing link in jasmonate signalling. Nature 448, 666–671. https://doi.org/10.1038/nature06006
Devoto, A., Turner, J.G. file:///C:/Users/Asus_Pro/Google D.G. paper/devoto2003. pdf, 2005. Jasmonate-regulated Arabidopsis stress signalling network. Physiol. Plant. 123, 161–172. https://doi.org/10.1111/j.1399-3054.2004.00418.x
FOEHSE, D., JUNGK, A., 1983. Influence of phosphate and nitrate supply on root hair formation of rape, spinach and tomato plants. Plant Soil 74, 359–368.
Goossens, J., Fernández-Calvo, P., Schweizer, F., Goossens, A., 2016. Jasmonates: signal transduction components and their roles in environmental stress responses. Plant Mol. Biol. 91, 673–689. https://doi.org/10.1007/s11103-016-0480-9
Huang, L.-F., Lin, K.-H., He, S.-L., Chen, J.-L., Jiang, J.-Z., Chen, B.-H., Hou, Y.-S., Chen, R.-S., Hong, C.-Y., Ho, S.-L., 2016. Multiple Patterns of Regulation and Overexpression of a Ribonuclease-Like Pathogenesis-Related Protein Gene, OsPR10a, Conferring Disease Resistance in Rice and Arabidopsis. PLoS ONE 11. https://doi.org/10.1371/journal.pone.0156414
Jungk, A., 2001. Root hairs and the acquisition of plant nutrients from soil. J. Plant Nutr. Soil Sci. 164, 121–129. https://doi.org/10.1002/1522-2624(200104)164:2<121::AID-JPLN121>3.0.CO;2-6
Livak, K.J., Schmittgen, T.D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25, 402–408. https://doi.org/10.1006/meth.2001.1262
Seo, P.J., Kim, M.J., Park, J.Y., Kim, S.Y., Jeon, J., Lee, Y.H., Kim, J., Park, C.M., 2010. Cold activation of a plasma membrane-tethered NAC transcription factor induces a pathogen resistance response in Arabidopsis. Plant J. 61, 661–671. https://doi.org/10.1111/j.1365-313X.2009.04091.x
Singh Gahoonia, T., Care, D., Nielsen, N.E., 1997. Root hairs and phosphorus acquisition of wheat and barley cultivars. Plant Soil 191, 181–188. https://doi.org/10.1023/A:1004270201418
Song, L., Yu, H., Dong, J., Che, X., Jiao, Y., Liu, D., 2016. The Molecular Mechanism of Ethylene-Mediated Root Hair Development Induced by Phosphate Starvation. PLOS Genet. 12, e1006194. https://doi.org/10.1371/journal.pgen.1006194
Thines, B., Katsir, L., Melotto, M., Niu, Y., Mandaokar, A., Liu, G., Nomura, K., He, S.Y., Howe, G.A., Browse, J., 2007. JAZ repressor proteins are targets of the SCFCOI1complex during jasmonate signalling. Nature 448, 661–665. https://doi.org/10.1038/nature05960
To, H.T.M., Nguyen, H.T., Dang, N.T.M., Nguyen, N.H., Bui, T.X., Lavarenne, J., Phung, N.T.P., Gantet, P., Lebrun, M., Bellafiore, S., Champion, A., 2019. Unraveling the Genetic Elements Involved in Shoot and Root Growth Regulation by Jasmonate in Rice Using a Genome-Wide Association Study. Rice 12, 69. https://doi.org/10.1186/s12284-019-0327-5
Wasternack, C., Hause, B., 2013. Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany. Ann. Bot. 111, 1021–1058. https://doi.org/10.1093/aob/mct067