rbcL and rpoL gene sequences of Panax vietnamensis var. fuscidiscus and Panax vietnamensis, the background for identification and comparison

Authors

  • Nguyen Thi Phuong Trang Viện Sinh thái và Tài nguyên Sinh vật (IEBR) ntptrang@yahoo.com nptrang@gmail.com Hongmai1304@gmail.com
  • Nguyen Thi Hong Mai Viện Sinh thái và Tài nguyên Sinh vật (IEBR) Hongmai1304@gmail.com
  • Zhuravlev Yury N
  • Reunova Galina D

DOI:

https://doi.org/10.15625/0866-7160/v39n1.7870

Keywords:

Panax vietnamensis var. fuscidiscus, Panax vietnamensis, rbcL, rpoB, chloroplast DNA

Abstract

The Panax vietnamensis var. fuscidiscus samples collected at Phong Tho district, Lai Chau Province have morphological characteristics very similar with those of Panax vietnamensis distributed in Ngoc Linh mountain at Quang Nam and Kon Tum provinces that hinders the identification of these taxons. To supply the information to identify two species and know the genetic distance of these species with other Panax species, we sequenced rbcL and rpoB genes that are among DNA barcoding markers recommended for plant species identification. In the rbcL gene of 700 bp in length, we found 9 nucleotide differences between Panax vietnamensis var. fuscidiscus and Panax vietnamensis and the genetic similarity was 98,8%, while in the rpoB gene of 500 bp in length, only 2 nucleotide differences were discovered and the genetic distance was 0,4%. The sequences of rbcL and rpoB of Panax vietnamensis var. fuscidiscus and Panax vietnamensis were submitted to Genbank with the accession numbers KT194325.1, KT194324.1 and KT154685.1, KT154686.1 respectively.

 

Citation: Nguyen Thi Phuong Trang, Nguyen Thi Hong Mai, Zhuravlev Yury N., Reunova Galina D., 2017. rbcL and rpoB gene sequences of Panax vietnamensis var. fuscidiscus and panax vietnamensis, the background for identification and comparison. Tap chi Sinh hoc, 39(1): 80-85. DOI: 10.15625/0866-7160/v39n1.7870

*Corresponding author: ntptrang@yahoo.com.

Received 11 March 2016, accepted 20 March 2017

 

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Author Biographies

Nguyen Thi Phuong Trang, Viện Sinh thái và Tài nguyên Sinh vật (IEBR) ntptrang@yahoo.com nptrang@gmail.com Hongmai1304@gmail.com

Làm việc tài phòng hệ thống học phân tử và di truyền bảo tồn

A11, viện Sinh thái và Tài nguyên sinh vật, số 18 hoàng Quốc Việt , Cầu Giấy, Hà Nội

nptrang@gmail.com

Nguyen Thi Hong Mai, Viện Sinh thái và Tài nguyên Sinh vật (IEBR) Hongmai1304@gmail.com

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Published

07-12-2016

How to Cite

Phuong Trang, N. T., Hong Mai, N. T., Yury N, Z., & Galina D, R. (2016). rbcL and rpoL gene sequences of Panax vietnamensis var. fuscidiscus and Panax vietnamensis, the background for identification and comparison. Academia Journal of Biology, 39(1), 80–85. https://doi.org/10.15625/0866-7160/v39n1.7870

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