Rapid micropropagation of Begonia tuberous by young leaf tissue culture and acclimatization in greenhouse

Abstract

In this paper, the protocol for the rapid micropropagation of Begonia tuberous are presented.

The shoot regeneration was carried out on 1/2MS medium, plus 0.7 mg/l BA and 0.03 mg/l NAA. Furthermore, the callus formation was obtained on 1/2MS medium supplemented with 0.7 mg/l BA and 0.1 mg/l NAA.

For the shoot multiplication, single shoots were cultured on MS medium, plus 1 mg/l BA and 0.3 mg/l NAA.

The root formation was obtained on 1/2MS medium, plus 0.5 mg/l NAA and 0.3  mg/l IBA.

To increase the growth and development possibility of Begonia tuberous in the greenhouse, we can use some chemical fertilizers (nitrophoska, Grow more, super) at a suitable dosage.