Micropropagation of wasabi plant (Wasabia japonica Matsumura) by shoot apex culture

Authors

  • Cao Dinh Hung VAST
  • Nguyen Tri Minh
  • Nguyen Trung Ai
  • Nguyen Thi Dieu Huong

DOI:

https://doi.org/10.15625/0866-7160/v25n3.6847

Abstract

The mass propagation technique by shoot apex culture of the wasabi plant (Wasabia japonica Matsumura) was established as follows:

1. Shoot apexes isolated from rhizomes were used as explants. In order to regenerate vigorous shoots, the explants were cultured on the half-strength MS basal medium supplemented with antibiotics.

2. For optimal multiple-shoots, the explants were cultured on the half-strength MS basal medium supplemented with a combination of BA at 0.1 mg/l and NAA at 0.02 mg/l within 30 days of culture. The addition of the activated charcoal at 0.1g/l to the root regeneration medium, which had been supplemented with 0.1mg/l of NAA, was necessary.

The result also showed that, after 8 months of transplantation and acclimatization, the plants with survival rate of 90-97% began to shoot and grew well under the greenhouse conditions.

Through this study, a large number of synchronous and bacteria-free plantlets were simultaneously obtained to meet the great demand of the local farmers and the japanese-owned enterprises in Dalat region for growing and processing the wasabi plant.

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Published

02-09-2015

How to Cite

Hung, C. D., Minh, N. T., Ai, N. T., & Dieu Huong, N. T. (2015). Micropropagation of wasabi plant (Wasabia japonica Matsumura) by shoot apex culture. Academia Journal of Biology, 25(3), 110–113. https://doi.org/10.15625/0866-7160/v25n3.6847

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