Analysis of genetic diversity of Ven dog breed based on microsatellite markers

Dieu Thuy Nguyen Thi; Cong Trieu Le; Phuong Loan Huynh Thi; Tra Mi Bui Thi; Huy Tuong Nguyen; Anh Khoa Do Vo

doi:10.15625/2615-9023/20446

Author affiliations

Authors

Nguyen Thi Dieu Thuy Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Le Cong Trieu Soc Trang Vocational College, 176 Nam Ky Khoi Nghia, Soc Trang, Vietnam
Huynh Thi Phuong Loan Can Tho University, Can Tho, Vietnam
Bui Thi Tra Mi Nong Lam University, Thu Duc, Ho Chi Minh City, Vietnam
Nguyen Huy Tuong Vinh Long College, Vinh Long, Vietnam
Do Vo Anh Khoa Vietnam National University of Forestry, Ha Noi, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/20446

Keywords:

Ven indigenous dogs, microsatellite marker, genetic diversity, inbreeding.

Abstract

Microsatellite (MS) is a genetic marker widely used in the studies of pedigree, individual identification, gene mapping, and genetic diversity within and between populations. The genetic diversity of the indigenous Ven dog breed, along with two imported dog breeds, the Berger and Poodle dogs raised in Vietnam, was analyzed based on seven microsatellite markers. A total of 32 alleles, an average number of alleles/loci of 4.6 were observed across 80 samples of the three dog breeds. The overall polymorphic information content (PIC) was 0.67 representing the quality of selected MS markers. Genetic diversity indices (Ho, He, Fit, Fst) showed the phenomenon of inbreeding between individuals in the Ven dog population. The genetic similarity level and phylogenetic tree also reasonably reflect the genetic relationship between the three analyzed dog breeds, in which the Ven dog showed a higher genetic distance compared to two imported dog breeds.

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