Associations of \(\textit{OTUBAIN-1, STAT, SHP}\) and \(\textit{NF-KB2}\) expression with clinical features in Non-Hodgkin’s lymphoma patients

Trang Do Thi; Ha Nguyen Trong; Xuan Thi Nguyen

doi:10.15625/2615-9023/19064

Author affiliations

Authors

Do Thi Trang 103 Hospital, Vietnam Military Medical University, 261 Phung Hung, Ha Dong, Ha Noi, Vietnam
Nguyen Trong Ha Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Nguyen Thi Xuan Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam https://orcid.org/0000-0003-3494-5136

DOI:

https://doi.org/10.15625/2615-9023/19064

Keywords:

NF-kB2, Otubain-1, non- Hodgkin’s lymphoma and STAT5.

Abstract

Non-Hodgkin's lymphoma (NHL) is a group of lymphoproliferative disorders characterized by the abnormal proliferation and accumulation of lymphocytes in the lymphatic system. An ovarian tumor domain containing ubiquitin aldehyde binding protein 1 (Otubain-1) is a deubiquitinating enzyme that cleaves ubiquitin or ubiquitin-like molecules and is expressed in various human tissues. The pathogenesis of NHL is associated with activations of the nuclear transcription factor (NF-κB) and signal transducer and activator of transcription proteins (JAK/STAT) signaling pathways. In this study, all the expressions of Otubain-1, NF-κB2, SHPs and STATs genes, the concentrations of cytokines IL-1β, IL-6 and TNF-α and clinical features in NHL patients were examined. To the end, gene expression levels of 82 NHL patients and 56 healthy individuals were determined by quantitative real time RT-PCR and secretion of cytokines by ELISA. As a result, concentrations of IL-6 and TNF-α in the patient group were found higher than in the healthy individuals and patients with higher LDH concentrations in the clinical cutoff, 280 U/L showed increased concentrations of AST, ALT and GGT than those with normal LDH concentrations. Interestingly, NHL patients with high Otubain-1 expression had significant elevations of GGT and ferritin concentrations as well as STAT-5 expression compared to those with low Otubain-1 expression. In conclusion, the present study indicates that up-regulation of Otubain-1 led to activation of STAT5 in lymphoma cells and liver dysfunction and metabolic syndrome in NHL patients.

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