Genetic diversity of mitochondrial DNA D-loop sequence in Bang Troi chicken breed

Nguyen Thi Dieu Thuy, Dinh Thi Ngoc Thuy, Mai Thi Thanh Nga
Author affiliations


  • Nguyen Thi Dieu Thuy Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam; Graduated University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
  • Dinh Thi Ngoc Thuy Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
  • Mai Thi Thanh Nga North East College of Agriculture and Forestry, Quang Ninh province, Vietnam



Bang Troi chicken breed, mtDNA D-loop sequence, genetic diversity, haplotype, phylogeny.


Bang Troi chicken (GBT) is a local chicken that is free-gardening raised in Quang Ninh province. A 1,268 bp of mitochondrial DNA (mtDNA) D-loop of 17 Bang Troi chicken individuals was sequenced and analyzed. Comparative multiple sequence alignment revealed 17 base substitutions within 17 Bang Troi chicken individuals. Bang Troi chicken had a relatively high level of genetic diversity, expressed in the number of 13 haplotypes, haplotype diversity of 0.949. The Tajima’s test indicated that the analysed population in balancing selection with a D-value of 2.39. A phylogenetic tree based on 455 bp of hypervariable mtD-loop sequence classified 17 Bang Troi chicken individuals into three clades A, B, and E, which are common groups of Vietnamese and Asia indigenous chickens. This result provides the first genetic information and maternal lineages of Bang Troi chickens and it can support for registration of local chicken breeds.


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How to Cite

Nguyen, T. T. D., Dinh, T. N. T., & Mai, T. T. N. (2024). Genetic diversity of mitochondrial DNA D-loop sequence in Bang Troi chicken breed. Academia Journal of Biology, 46(1), 49–54.