Isolation and properties of endophytic bacteria and actinomycetes of <i>Catharanthus roseus</i> (L) G. Don grown in Nha Trang, Vietnam

My Linh Tran; Thi Hoe Pham; Chi Mai Nguyen; Khac Ban Ninh; Huong Giang Vu; Tuong Van Nguyen

doi:10.15625/2615-9023/18984

Author affiliations

Authors

Tran My Linh Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Pham Thi Hoe Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Nguyen Chi Mai Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Ninh Khac Ban Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Vu Huong Giang Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Nguyen Tuong Van VNTEST Institute for Quality Testing and Inspection, Vietnam Union of Science and Technology Associations, 53 Nguyen Du, Ha Noi, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/18984

Keywords:

Catharanthus roseus, endophytic bacteria, actinomycetes, extracellular enzyme.

Abstract

Endophytic microbes of medicinal plants are known to involve the synthesis of several bioactive compounds, which promote plant growth and resistance to diseases. In order to investigate endophytes in the important medicinal plant Catharanthus roseus, 16 endophytic microorganism strains were isolated from root samples of C. roseus var. roseus and C. roseus var. ocellatus naturally growing in coastal areas of Nha Trang, Vietnam. Based on morphological characteristics and 16S rDNA gene marker sequences, four actinomycetes of Streptomyces and Microbiospora genera and twelve bacterial strains belonging to Bacillus, Enterobacter, Pseudomonas, Panenibacillus and Rhizobium genera were identified. Analysis of the extracellular enzyme activities of actinomycetes strains indicated that Streptomyces strains produced proteases, cellulases, xylanases and amylases and Microbiospora strains exhibited protease and cellulase activities. The results demonstrated the diversity of endophytes in the roots of C. roseus plants and their potential extracellular enzyme activities for further application.

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References

Anjum N., Chandra R., 2019. Endophytic bacteria of Catharanthus roseus as an alternative source of vindoline and application of response surface methodology to enhance its production. Arch. Biol. Sci., 71(1): 27–38.

Eida M. F. Nagaoka T., Wasaki J., Kouno K., 2013. Phytate degradation by fungi and bacteria that inhabit sawdust and coffee residue composts. Microbes Environ. 28(1): 71–80.

Glick B. R., 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol. Res. 169: 30–39.

Hawar S. N., 2022. Extracellular enzyme of endophytic fungi isolated from Ziziphus spina leaves as medicinal plant. International Journal of Biomaterials, 2022: 2135927.

Holtz C., Kaspari H., Klemme J. H., 1991. Production and properties of xylanases from thermophilic actinomycetes. Antonie Van Leeuwenhoek, 59(1): 1–7.

Jasmine D. J., Agastian P., 2013. In vitro antioxidant activity and in vivo alpha glucosidase activity of endophytic actinomycetes isolated from Catharanthus roseus (l.) G. Don. J. Pharm. Res., 6(6): 674–678.

Joseph B., Priya R. M., 2011. Bioactive Compounds from Endophytes and their Potential in Pharmaceutical Effect: A Review. Am. J. Biochem. Mol. Biol., 1(3): 291–309. doi: 10.3923/ajbmb.2011.291.309

Kafur A., Basheer K. A., 2011. Isolation of endophytic actinomycetes from Catharanthes roseus (L.) G. Don leaves and their antimicrobial activity. Iran. J. Biotechnol., 9(4): 302–306.

Kumar A., Patil D., Rajamohanan P. R., Ahmad A., 2013. Isolation, purification and characterization of vinblastine and vincristine from endophytic fungus Fusarium oxysporum isolated from Catharanthus roseus. PloS one, 8(9): e71805.

Kumar M., Kumar P., Das P., Solanki R., Kapur M. K., 2020 Potential applications of extracellular enzymes from Streptomyces spp. in various industries. Arch Microbiol., 202(7): 1597–1615.

Kuriakose G. C., Palem P. P., Jayabaskaran C., 2016. Fungal vincristine from Eutypella spp-CrP14 isolated from Catharanthus roseus induces apoptosis in human squamous carcinoma cell line-A431. BMC Complementary Altern. Med., 16(1): 1–8.

Laura M. C., Rachel A. C., Martin W., Jasna K., 2022. Keeping up with the Bacillus cereus group: taxonomy through the genomics era and beyond, Crit. Rev. Food Sci. Nutr., 62(28): 7677–7702.

Li J., Zhao G. Z., Chen H. H., Wang H. B., Qin S., Zhu W. Y., Li W. J., 2008. Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in the rainforest. Lett. Appl. Microbiol., 47(6): 574–580.

Matsumoto A., Takahashi Y., Mochizuki M., Seino A., Iwai Y., Omura, S., 1998. Characterization of actinomycetes isolated from fallen leaves. Actinomycetologica, 12(1): 46–48. doi: 10.3209/saj.12_46

Moudi M., Go R., Yien C.Y.S., Nazre M., 2013. Vinca alkaloids. Int. J. Prev. Med., 4(11): 1231–1235.

Palem P. P., Kuriakose G. C., Jayabaskaran C., 2016. Correction: an endophytic fungus, Talaromyces radicus, isolated from Catharanthus roseus, produces vincristine and vinblastine, which induce apoptotic cell death. Plos one, 11(4): e0153111.

Petrini O., 1991. Fungal endophytes of tree leaves, Microbial Ecology of Leaves. Springer, New York, pp. 179–197.

Robl D., Mergel C. M., Costa P. D. S., Pradella J. G. D. C., Padilla G., 2019. Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation. Braz. Arch. Biol. Technol.: 62.

Sharma M., Dangi P., Choudhary M., 2014. Actinomycetes: source, identification, and their applications. Int. J. Curr. Microbiol. App. Sci., 3(2): 801–832.

Shutsrirung A., Chromkaew Y., Pathom-Aree W., Choonluchanon S., Boonkerd N., 2013. Diversity of endophytic actinomycetes in mandarin grown in northern Thailand, their phytohormone production potential and plant growth promoting activity. Soil Science and Plant Nutrition, 59(3): 322–330.

Sottomayor M., Ros Barceló A., 2005. The vinca alkaloids: from biosynthesis and accumulation in plant cells, to uptake, activity and metabolism in animal cells, Studies in natural products chemistry (bioactive natural products), Vol 33. Elsevier Science, Amsterdam, pp. 813–857.

Sousa C. D. S., Soares A. C. F., Garrido M. D. S., 2008. Characterization of streptomycetes with potential to promote plant growth and biocontrol. Sci. Agric.(Piracicaba, Braz.), 65(1): 50–55.

Tamura K., Stecher G., Peterson D., Filipski A., Kumar S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol. Biol. Evol., 30: 2725–2729.

Tan R. X., Zou W. X., 2001. Endophytes: a rich source of functional metabolites. Nat. Prod. Rep., 18(4): 448–459.

Thao P. T. H., Linh N. V. M., Lien N. T. H., Hieu N. V., 2016a. Biological characteristics and antimicrobial activity of endophytic Streptomyces sp. TQR12-4 isolated from elite Citrus nobilis cultivar Ham Yen of Vietnam. Int. J. Microbiol., 2016.

Thao P. T. H., Linh N. V. M., Lien N. T. H., Hieu N. V, 2016b. Endophytic Actinomyces Streptomyces parvulus HNR3X4 in Ha Noi - Dien Grapfruit Trees and the Potential to Biosynthetize Antimicrobial Substances. VNU Journal of Science: Earth and Environmental Sciences: 32(1S) (In Vietnamese with English summary).

Wachinger G., Bronnenmeier K., Staudenbauer W. L., Schrempf H., 1989. Identification of mycelium-associated cellulase from Streptomyces reticuli. Appl. Environ. Microbiol., 55(10): 2653–2657.

Wirth S., Ulrich A., 2002. Cellulose-degrading potentials and phylogenetic classification of carboxymethyl-cellulose decomposing bacteria isolated from soil. Syst. Appl. Microbiol., 25(4): 584–591.

Zhao S., Zhou N., Zhao Z. Y., Zhang K., Wu G. H., Tian C. Y., 2016. Isolation of endophytic plant growth-promoting bacteria associated with the halophyte Salicornia europaea and evaluation of their promoting activity under salt stress. Curr. Microbiol. 73: 574–581.