Chemical composition of \(\textit{Elsholtzia ciliata}\) (Thunb.) Hyland essential oil in Vietnam with multiple biological utilities: a survey on antioxidant, antimicrobial, anticancer activities

Luu Tang Phuc Khang, Nguyen Huu Tai, Cao Van Len, Nguyen Ngoc Thu Phuong, Nguyen Hoang Viet, Truong Vinh, Phan Thanh Bach, Tran Thi Phuong Dung, Nguyen Xuan Tong
Author affiliations


  • Luu Tang Phuc Khang Ho Chi Minh City University of Education, Ho Chi Minh City, Vietnam
  • Nguyen Huu Tai Ho Chi Minh City University of Education, Ho Chi Minh City, Vietnam
  • Cao Van Len Ho Chi Minh City University of Education, Ho Chi Minh City, Vietnam
  • Nguyen Ngoc Thu Phuong Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam
  • Nguyen Hoang Viet Can Tho University of Medicine and Pharmacy, Can Tho City, Vietnam
  • Truong Vinh International School Ho Chi Minh City, Ho Chi Minh City, Vietnam
  • Phan Thanh Bach Nong Lam University, Ho Chi Minh City, Vietnam
  • Tran Thi Phuong Dung Ho Chi Minh City University of Education, Ho Chi Minh City, Vietnam
  • Nguyen Xuan Tong Institute of Environmental Science, Engineering and Management, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Vietnam



Anticancer, antimicrobial, antioxidant, Elsholtzia ciliata, essential oil.


Elsholtzia ciliata (Thunb.) Hyland is commonly known as Vietnamese balm - a spicy, lemon-scented culinary herb in Asian cuisine, especially in Vietnam. The biological activities of E. ciliata essential oils (Ec EOs) in Vietnam have yet to be thoroughly studied and have received less attention than other species of genus Elsholtzia in the world. In this study, we evaluated the antioxidant, antimicrobial, and anticancer of Ec EOs and examined their chemical compositions. Fresh leaves of E. ciliata were hydro-distilled to yield essential oil of 0.82% dry weight, respectively. Gas chromatography-mass spectrometry (GC-MS) analyses revealed that Ec EOs principally possessed complex mixtures of monoterpenes and sesquiterpenes. (Z)-β-Farnesene (22.72%), neral (15.66%), geranial (15.62%), and β-ocimene (13.30%) were the major components of Ec EOs. In the antioxidant assay, the radical scavenging capacities of Ec EOs against DPPH were 26.55 g/L (IC50). In the antimicrobial assay, the evaluation of antimicrobial activity using the agar wells diffusion method showed that Ec EOs in all concentrations was active against the Gram-positive bacteria (Bacillus cereus, Bacillus subtilis, Staphylococcus aureus), Gram-negative bacteria (Proteus mirabilis, Escherichia coli, Klebsiella pneumoniae). In the anticancer assay, EcE Os can be toxic to Hep G2 cells with IC50 reaching 0.00204%.


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How to Cite

Luu Tang Phuc, K., Nguyen, H. T., Cao, V. L., Nguyen, N. T. P., Nguyen, H. V., Truong, V., Phan, T. B., Phuong Dung, T. T., & Xuan Tong, N. (2023). Chemical composition of \(\textit{Elsholtzia ciliata}\) (Thunb.) Hyland essential oil in Vietnam with multiple biological utilities: a survey on antioxidant, antimicrobial, anticancer activities. Academia Journal of Biology, 45(3), 99–110.