Biochemical and molecular adaptation of rice plants towards low phosphate condition
Author affiliations
DOI:
https://doi.org/10.15625/2615-9023/17087Keywords:
Low-phosphate-responsive genes, low-Pi sensitive variety, low-Pi tolerant variety, Oryza sativa, phosphate starvation, phosphate use efficiencyAbstract
Rice is the staple food for half of the world's population. Phosphorus is an essential element for rice plant growth, development and production; however, the availability of soluble phosphate for plant uptake is often limited due to the binding with cation in different pH environments. Therefore, the development of rice plant varieties, which can grow well under a limited phosphate environment, is an urgent need for agricultural development for biotechnology. This study reported two contrasting rice varieties' biochemical and molecular adaptation toward a low phosphate medium. The results show that the response of these two varieties is genotype-dependent. We observed a higher phosphate use efficiency in the low-Pi-sensitive G299 rice variety than in the low-Pi-tolerant G22 rice variety.
Moreover, the expression of five out of six investigated genes relating to the low Pi responsive pathway, including two purple acid phosphatase (PAP), phosphate starvation response 2 (PHR2), SPX (SYG1/PHO81/XPR1) domain-containing gene, phosphate transcription factor 1 (PTF1) and phosphate 1 (PHO1) were significantly higher in the low-Pi-sensitive accession than in the tolerant genotype. Impressively, 214 times more expression of the PHO1 was observed in the leaves of the G299 variety than in the G22 variety, followed by SPX, PTF1, PHR2, and PAP21 genes. Similarly, the PHO1 was induced at the highest level in the roots of the G299 variety, followed by PAP21, PHR2, PTF1, and SPX1 genes. These findings supply further information for elucidation of the mechanism behind the response of rice plants to low phosphate medium and the development of the low Pi nutrient happy rice varieties.
Downloads
Metrics
References
Abel S., Ticconi C. A., Delatorre C. A., 2002. Phosphate sensing in higher plants. Physiol Plant, 115: 1–8. http://dx.doi.org/10.1034/j.1399-3054.2 002.1150101.x
Balemi T., Negisho K., 2012. Management of soil phosphorus and plant adaptation mechanisms to phosphorus stress for sustainable crop production: A review. J Soil Sci Plant Nutr, 12: 547–561. http://dx.doi.org/10.4067/S0718-951620 12005000015
Bozzo G. G., Dunn E. L., Plaxton W. C., 2006. Differential synthesis of phosphate-starvation inducible purple acid phosphatase isozymes in tomato (Lycopersicon esculentum) suspension cells and seedlings. Plant Cell Environ, 29: 303–313. https://doi.org/10.1111/ j.1365-3040.2005.01422.x
Bustos R., Castrillo G., Linhares F., Puga M. I., Rubio V., Pérez J. P., Solano R., Leyva A., Ares J. P., 2010. A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis. PLOS Genet, 6: e1001102. https://doi.org/10.1111/j.1365-3040.2005.01422.x
Cao H. X., Zhang Z. Bin, Sun C. X., Shao H. B., Song W. Y., Xu P., 2009. Chromosomal location of traits associated with wheat seedling water and phosphorus use efficiency under different water and phosphorus stresses. Int J Mol Sci, 10: 4116–4136. https://doi.org/ 10.3390/ijms10094116
Cordell D., Drangert J. O., White S., 2009. The story of phosphorus: Global food security and food for thought. Glob Environ Chang, 19: 292–305. https://doi.org/10.1016/j.gloenvcha.2008.10.009
Cordell D., Rosemarin A., Schröder J. J., Smit A. L., 2011. Towards global phosphorus security: A systems framework for phosphorus recovery and reuse options. Chemosphere, 84: 747–758. https://doi.org/10.1016/j.chemosphere.2011.02.032
Gamuyao R., Chin J. H., Pariasca-Tanaka J., Pesaresi P., Catausan S., Dalid C., Slamet-Loedin I, Tecson-Mendoza E. M., Wissuwa M., Heuer S., 2012. The protein kinase Pstol1 from traditional rice confers tolerance of phosphorus deficiency. Nature, 488: 535–539. https://doi.org/10.1038/nature11346
Hammond J. P., Broadley M. R., White P. J., King G. J., Bowen H. C., Hayden R., Meacham M. C., Mead A., Overs T., Spracklen U. P., Greenwood D. J., 2009. Shoot yield drives phosphorus use efficiency in Brassica oleracea and correlates with root architecture traits. J Exp Bot, 60: 1953–1968. https://doi.org/ 10.1093/jxb/erp083
He Y., Liao H., Yan X., 2003. Localized supply of phosphorus induces root morphological and architectural changes of rice in split and stratified soil cultures. Plant Soil, 248:247–256. https://doi.org/ 10.1023/A:1022351203545
Hunter M. C., Smith R. G., Schipanski M. E., Atwood L. W., Mortensen D. A., 2017. Agriculture in 2050: Recalibrating targets for sustainable intensification. Bioscience, 67: 386–391. https://doi.org/10.1093/biosci/bix010
Kirkby E. A., Johnston A. E., 2008. Soil and fertilizer phosphorus in relation to crop nutrition. The Ecophysiology of Plant-Phosphorus Interactions, 177–223. https://doi.org/10.1007/978-1-4020-8435-5_9
Liu D., 2021. Root developmental responses to phosphorus nutrition. J Integr Plant Biol, 63:1065–1090. https://doi.org/ 10.1111/jipb.13090
Livak K. J., Schmittgen T. D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 25: 402–408. https://doi.org/10.1006/meth.2001.1262
Mai N. T. P., Mai C. D., Nguyen H. Van, Le Q. K., Duong V. L., Tran T. A., To T. M. H., 2021. Discovery of new genetic determinants of morphological plasticity in rice roots and shoots under phosphate starvation using GWAS. J Plant Physiol, 257. https://doi.org/10.1016/j.jplph.2020. 153340
Mehra P., Pandey B. K., Giri J., 2017. Improvement in phosphate acquisition and utilization by a secretory purple acid phosphatase (OsPAP21b) in rice. Plant Biotechnol J, 15: 1054–1067. https://doi.org/10.1111/pbi.12699
Qundan L., Zhong Y., Wang Y., Wang Z., Zhang L., Shi J., Wu Z., Liu Y., Mao C., Yi K., Wu P., 2014. Spx4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2 in rice. Plant Cell, 26: 1586–1597. https://doi.org/10.1105/ tpc.114.123208
Secco D., Baumann A., Poirier Y., 2010. Characterization of the rice PHO1 gene family reveals a key role for OsPHO1;2 in phosphate homeostasis and the evolution of a distinct clade in dicotyledons. Plant Physiol, 152: 1693–1704. https://doi.org/ 10.1104/pp.109.149872
Stefanovic A., Arpat A. B., Bligny R., Gout E., Vidoudez C., Bensimon M, Poirier Y., 2011. Over-expression of PHO1 in Arabidopsis leaves reveals its role in mediating phosphate efflux. Plant J, 66: 689–699. https://doi.org/10.1111/j.1365-313X.2011.04532.x
Stefanovic A., Ribot C., Rouached H., Wang Y., Chong J., Belbahri L., Delessert S., Poirier Y., 2007. Members of the PHO1 gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways. Plant J, 50: 982–994. https://doi.org/10.1111/j.1365-313X.20 07.03108.x
To H. T. M., Le K. Q., Nguyen V. H., Duong V. L., Kieu T. H., Chu T. Q. A., Tran P. T, Mai T. P. N., 2020. A genome-wide association study reveals the quantitative trait locus and candidate genes that regulate phosphate efficiency in a Vietnamese rice collection. Physiol Mol Biol Plants, 26: 2267–2281. https://doi.org/10.1007/s12298-020-00 902-2
Vigueira C. C., Small L. L., Olsen K. M., 2016. Long-term balancing selection at the Phosphorus Starvation Tolerance 1 (PSTOL1) locus in wild, domesticated and weedy rice (Oryza). BMC Plant Biol, 16:1–10. https://doi.org/10.1186/s12870-016-0783-7
Wang C., Ying S., Huang H., Li K., Wu P., Shou H., 2009. Involvement of OsSPX1 in phosphate homeostasis in rice. Plant J, 57: 895–904. https://doi.org/10.1111/j.1365-313X.20 08.03734.x
White P. J., Hammond J. P., 2008. Phosphorus nutrition of terrestrial plants. Plant Ecophysiology: 51–81. http://doi.org/10.1007/978-1-4020-8435-5_4
Yamamoto E., Yonemaru J. ichi, Yamamoto T., Yano M., 2012. OGRO: The overview of functionally characterized genes in rice online database. Rice, 5: 1–10. https://doi.org/10.1186/1939-8433-5-26
Yang Y., Zhu K., Xia H., Chen L., Chen K. 2014. Comparative proteomic analysis of indica and japonica rice varieties. Genet Mol Biol, 37: 652–661. https://doi.org/ 10.1590/S1415-47572014005000015
Yi K., Wu Z., Zhou J., Du L., Guo L., Wu Y., Wu P., 2005. OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice. Plant Physiol, 138:2087–2096. http://doi.org/10.1104/pp.105.063115
Zhang Q., Wang C., Tian J., Li K., Shou H., 2011. Identification of rice purple acid phosphatases related to phosphate starvation signalling. Plant Biol, 13: 7–15. https://doi.org/10.1111/j.1438-8677.20 10.00346.x
Zhong Y., Wang Y., Guo J., Zhu X., Shi J., He Q., Liu Y., Wu Y., Zhang L., Lv Q., Mao C., 2018. Rice SPX6 negatively regulates the phosphate starvation response through suppression of the transcription factor PHR2. New Phytol, 219: 135–148. https://doi.org/ 10.1111/nph.15155
Zhou J., Jiao F. C., Wu Z., Li Y., Wang X., He X., Zhong W., Wu P., 2008. OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants. Plant Physiol, 146:1673–1686. https://doi.org/10.1104/pp.107.111443
Downloads
Published
How to Cite
Issue
Section
