Polyhydroxybutyrate accumulation of \(\textit{ Bacillus}\) sp. strains using water hyacinth hydrolysate as a carbon source

Authors

  • Kim Thoa Nguyen Institute of Biotechnology, VAST, Vietnam
  • Thi Hong Nhung Lai Institute of Biotechnology, VAST, Vietnam
  • Thi Da Nguyen Institute of Biotechnology, VAST, Vietnam
  • Thi Huyen La Institute of Biotechnology, VAST, Vietnam
  • Hai Yen Dao Institute of Chemistry, VAST, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/16887

Keywords:

Polyhydroxybutyrate (PHB), Bacillus, water hyacinth, lignocellulose hydrolysis, bioplastic

Abstract

The microbial cells can store energy in the form of polyhydroxyalkanoates (PHAs) while the nutrient sources are exhausted but have an excess of carbon sources. Poly(3-hydroxybutyrate) (PHB) was known as one of the most common PHA. Currently, PHB is assessed as a potential alternative to petroleum-based plastics such as HDPE, PP. In addition, PHB can be obtained from different microbes through the fermentation of renewable and sustainable materials such as waste from food or cassava starch industry, and other agricultural by-products. Although the spread of water hyacinth (Eichhornia crassipes) is becoming a problem in many provinces, it still is considered an opulent biomass source. Besides the application in the removal of heavy metals from wastewater, or making animal feed and fertilizer, water hyacinth can be converted into a carbon source used in microbial fermentation. This paper indicates the PHA synthesis ability of 28 bacterial strains which were isolated from soybean-growing soil samples and the Cau Dien Waste-treatment Plant’s mud samples. Based on their PHA accumulation capability while using C5 and C6 sugars as carbon sources, Bacillus sp. AI 10 and Bacillus sp. CRCXL 2.2 were chosen to synthesize PHA using the water hyacinth hydrolysate as a carbon source. Pretreated water hyacinth biomass using Ca(OH)2 was subjected to enzymatic hydrolysis with a suitable ratio of Cellic→CTec2 and Cellic→HTec2, which resulted in a 409.5 mg total reducing sugars/g pretreated biomass. After 48 hours of fermentation, the dry biomass and accumulated PHA amount from Bacillus sp. AI 10 and Bacillus sp. CRCXL 2.2 were 4.79 g/L, 51.2% and 3.84 g/L, 34.7%, respectively. The Fourier transform infrared spectroscopy (FTIR) spectra of both strains’ PHA structure showed that they can accumulate the homopolymer of PHB. From these results, it is possible to produce PHB by microorganisms from water hyacinth biomass, and participate in the circular bio-economic chain.

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References

Bajpai P., 2016. Pretreatment of Lignocellulosic Biomass for Biofuel Production. Springer.

Belgacem M. N., Gandini A., 2008. Monomers, Polymers and Composites from Renewable Resources, 1st ed.; Elsevier Science: Amsterdam, The Netherlands.

Bergey D. H., Whitman W. B., De Vos P., Garrity G. M., Jones D., 2009. Bergey's Manual of Systematic Bacteriology: Vol. 3. New York: Springer.

Bradford M. M., 1976. A Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal. Biochem., 72(1–2): 248–254.

Das A., Ghosh P., Paul T., Ghosh U., Pati B. R., Mondal K. C., 2016. Production of bioethanol as useful biofuel through the bioconversion of water hyacinth (Eichhornia crassipes). 3 Biotech., 6(1): 70

Doan H. T., Nguyen P. T. M., Tran T. T., Nguyen T. K., Tran M. D., Nguyen D. B., 2021. Optimizing lime pretreatment of rice straw for biolipid production using oleaginous microorganisms. Chemosphere., 269: 129390.

Doan V. T., and Vu T. A., 2021. Bioconversion of Crude Fish Oil Into Poly-3-hydroxybutyrate by Ralstonia sp. M91. Appl. Biochem. Microbiol., 57: 219–225.

Doan V. T., Dam N. M., Tran T. L., Sudesh K., 2019. Utilization of waste fish oil and glycerol as carbon sources for polyhydroxyalkanoate production by Salinivibrio sp. M318. Int. J. Biol. Macromol., 141: 885-892.

Doan V. T., Nguyen T. C., Hatti-Kaul R., 2021. Polyhydroxyalkanoate production from rice straw hydrolysate obtained by alkaline pretreatment and enzymatic hydrolysis using Bacillus strains isolated from decomposing straw. Bioresour. Bioprocess., 8: 98.

Doan Van Thuoc, Tran Thi Loan, and Pham Thi Hong Hoa, 2020. Production of Poly(3-hydroxybutyrate) from raw cassava starch by Bacillus megaterium D8. Natural Science – HNUE Journal of Science, 65(10): 73-81.

Ganguly A., Chatterjee P. K., Dey A., 2012. Studies on Ethanol Production from Water Hyacinth-A Review. Renew. Sust. Energ. Rev., 16: 966-972

Grigore M. E., Grigorescu R. M., Iancu L., Ion R. -M., Zaharia C., Andrei E. R., 2019. Methods of Synthesis, Properties and Biomedical Applications of Polyhydroxyalkanoates: A Review. J. Biomater. Sci. Polym. Ed., 30, 695–712;

Law J. H., Slepecky R. A., 1961. Assay of poly-beta-hydroxybutyric acid. J. Bacteriol., 82(1): 33-36.

Li J., Yang Z., Zhang K., Liu M., Liu D., Yan X., Si M., Shi Y., 2021. Valorizing waste liquor from dilute acid pretreatment of lignocellulosic biomass by Bacillus megaterium B‑10. Ind. Crops. Prod., 161:113160.

Manivannan A., Narendhirakannan R. T., 2015. Bioethanol production from aquatic weed water hyacinth (Eichhornia crassipes) by yeast fermentation. Waste. Biomass. Valori. 6: 209-216.

McAdam B., Brennan-Fournet M., McDonald P., Mojicevic M., 2020. Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics. Polymers (Basel)., 12(12): 2908.

Miller G. L., 1959. Use of Dinitrosalicylic Acid Reagent For The Determination of Reducing Sugars. Anal. Chem., 31: 426-428.

Modig T., Liden G., Taherzadeh M. J., 2002. Inhibition Effects of Furfural on Alcohol Dehydrogenase, Aldehyde Dehydrogenase and Pyruvate Dehydrogenase. Biochem. J., 363(Pt.3): 769-776

Nguyen Thi Da, 2021. Scientific report on “Investigation on the recombinant bioplastic producing strains for bioconversion of fish solid waste to bioplastic”. Project ĐT.08.19/CNSHCB, managed by “Development and application of biotechnology in the field of processing industry till 2020” Project, supported by Ministry of Trade and Industry (In Vietnamese).

Nguyen Thi Da, Nguyen Trong Linh, Nguyen Thu Trang, Tran Manh Hai, La Thi Huyen, 2020. Screenning and determination of polyhydroxyalkanoate production by Bacillus sp. strains. Vietnam Journal of Food Control, 3(3): 165-172.

Ostle A. G., Holt J. G., 1982. Nile Blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl. Environ. Microbiol., 44: 238-241.

Perry D.L., 2011. Handbook of Inorganic Compounds. CRC press

Pham Thanh Ha, 2010. Scientific report on “Seeking out inexpensive materials for biopolymer production from a mutant Alcaligenes latus strain”. VAST project 2008-2009. (In Vietnamese).

Rao U., Ravichandran S., Sehgal P.K., 2010. Biosynthesis and Biocompatibility of P(3HB-Co-4HB) Produced by Cupriavidus Necator from Spent Palm Oil. Biochem. Eng. J., 49: 13–20.

Sindhu R., Ammu B., Binod P., Deepthi S. K., Ramachandran K. B., Soccol C. R., Pandey A., 2011. Production and Characterization of Poly-3-Hydroxybutyrate from Crude Glycerol by Bacillus sphaericus NII 0838 and Improving Its Thermal Properties by Blending with Other Polymers. Brazilian. Arch. Biol. Technol., 54: 783–794.

Sindhu R., Silviya N., Binod P., Pandey A., 2013. Pentose‑rich hydrolysate from acid pretreated rice straw as a carbon source for the production of poly‑3‑hydroxybutyrate. Biochem. Eng. J., 78: 67–72.

Steinbüchel A., 2001. Perspectives for Biotechnological Production and Utilization of Biopolymers: Metabolic Engineering of Polyhydroxyalkanoate Biosynthesis Pathways as a Successful Example. Macromol. Biosci. 1: 1–24;

Ushani U., Sumayya A. R., Archana G., Rajesh Banu J., Jinjin Dai., 2020. Chapter 10 - Enzymes/biocatalysts and bioreactors for valorization of food wastes. In Food Waste to Valuable Resources. Application and Managemenet. pp: 211-233.

Verlinden R. A. J., Hill D. J., Kenward M. A., Williams C. D., Radecka I., 2007. Bacterial Synthesis of Biodegradable Polyhydroxyalkanoates. J. Appl. Microbiol., 102: 1437–1449.

Xu J., Wang Z., Sharma-Shivappa R. R., Cheng J. J., 2011. Enzymatic hydrolysis of switchgrass and coastal Bermuda grass pretreated using different chemical methods, BioRes., 6(3): 2990-3003.

Zhang G. C., Liu J. J., Kong I. I., Kwak S., Jin Y. S., 2015. Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion. Curr. Opin. Chem. Biol., 29: 49–57.

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Published

28-09-2022

How to Cite

Nguyen, K. T., Nhung Lai, T. H. ., Nguyen, T. D., La, T. H., & Yen Dao, H. (2022). Polyhydroxybutyrate accumulation of \(\textit{ Bacillus}\) sp. strains using water hyacinth hydrolysate as a carbon source. Academia Journal of Biology, 44(3), 35–45. https://doi.org/10.15625/2615-9023/16887

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