Isolation and characterization of a gene encoding farnesyl diphosphate synthase from \(\textit{Panax vietnamensis}\) Ha et Grushv


  • Nguyen Van Giang Institute of Genome Research, VAST, Vietnam
  • Luu Han Ly Institute of Genome Research, VAST, Vietnam
  • Pham Le Bich Hang Institute of Genome Research, VAST, Vietnam
  • Le Thi Thu Hien Institute of Genome Research, VAST, Vietnam



Panax vietnamensis, ginsenoside biosynthesis, triterpenoid saponins, farnesyl diphosphate synthase, farnesyl pyrophosphate synthase, gene expression


Panax vietnamensis Ha et Grushv. is a species of the genus Panax native to Central Vietnam, containing a family of triterpene saponins named ginsenosides. This group of biomolecules possesses valuable therapeutic properties against cancer, hepatitis, diabetes, inflammation as well as stress and anxiety. Farnesyl diphosphate synthase (FPS) is a key enzyme participating in the ginsenoside biosynthesis pathway. In this study, a FPS gene from P. vietnamensis (PvFPS) was isolated and characterized. The PvFPS cDNA contained an open reading frame of 1032 bp, encoding a polypeptide chain of 342 amino acid residues. Nucleotide sequence comparison showed that FPS was highly conserved among most species, with two Aspartate-rich motifs responsible for product chain length determination strongly sustained. PvFPS was closely related to those of the same genera and order and differed from those from other kingdoms. PvFPS expression was detected at a greater level in root tissues than in leaves in all ages. Our findings provided information concerning the properties of a crucial gene in the ginsenoside biosynthesis, thus enhancing our understanding of this important pathway.


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Author Biography

Le Thi Thu Hien, Institute of Genome Research, VAST, Vietnam

Genome Biodiversity Laboratory


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How to Cite

Giang, N. V., Ly, L. H., Hang, P. L. B., & Hien, L. T. T. (2021). Isolation and characterization of a gene encoding farnesyl diphosphate synthase from \(\textit{Panax vietnamensis}\) Ha et Grushv. Academia Journal of Biology, 43(4), 119–128.