Trematode larval infections in snails collected from aquaculture ponds in Ha Noi and Yen Bai, Vietnam


  • Nguyen Manh Hung Institute of Ecology and Biological Resources, VAST, Vietnam



Opisthorchiidae, snail hosts, trematode larvae, VAC ponds.


The hot-spots of transmission of food-borne zoonotic trematodes in northern Vietnam have been known as the integrated fish-livestock (VAC- Garden, Fish pond, Poultry shed) ponds. A cross-sectional study was conducted in Ha Noi capital and Yen Bai province to investigate the trematode larval infections (cercariae) in freshwater snails in these areas. Snails were collected from 35 VAC ponds, including 25 grow-out and 10 nursery ponds. A total of 13,895 specimens of 12 snail species were examined for cercariae shedding. Six of 12 snail species including Melanoides tuberculata, Tarebia granifera, Radix auriculata, Gyraulus convexiusculus, Bithynia fuchsiana and Parafosarulus manchouricus, shed cercariae. Seven cercarial types were detected. The amount of snails and the prevalent infection in nursery ponds were 1.14 and 1.3 times higher than in grow-out ponds, respectively. Thus, VAC ponds in northern Vietnam are still the hot-spot for trematode larval infection.


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Author Biography

Nguyen Manh Hung, Institute of Ecology and Biological Resources, VAST, Vietnam


Brandt A. M. R., 1974. The non-marine aquatic Mollusca of Thailand. Archiv Molluskenkund., 105: 1–247.

Bui T. D., Madsen H., Dang T. T., 2010. Distribution of freshwater snails in family-based VAC ponds and associated waterbodies with special reference to intermediate hosts of fish-borne zoonotic trematodes in Nam Dinh provinces, Vietnam, Acta Trop., 116: 15–23.

Chai J. Y., Shin E. H., Lee S. H., Rim H. J., 2009. Foodborne intestinal flukes in Southeast Asia. Korean J Parasitol., 47: 69–102.

Clausen J. H., Madsen H., Murrell K. D., Van P. T., Ha N. T. T., Dung D. T., Anh N. T. L., Hung N. M., Dalsgaard A., 2012. Prevention and control of fish-borne zoonotic trematodes in fish nurseries, Vietnam, Emerging Infect Dis., 18: 1438–1445.

Dang N. T., 1980. Identification of Freshwater Invertebrates in Northern Vietnam. Science and Technology Publisher, pp. 440–490. (In Vietnamese).

Doanh P. N., Le N. T., 2005. Identifical characteristics of trematode cercaria groups and differentiation of cercaria of Fasciola gigantica Cobbold, 1885 in snails Lymnaea in Vietnam. Academia Journal of Biology (Tap chi Sinh hoc), 27(3A): 37–40. (in Vietnamese).

Doanh P. N., Hien H. V., Dung B. T., Loan H. T., 2019. Infection status and molecular identification of digenean cercariae in snails in Kim Son district, Ninh Binh province and Ba Vi district, Hanoi. Academia Journal of Biology (Tap chi Sinh hoc), 41(3): 31–38. (in Vietnamese).

Dung D. T., De N. V., Waikagul J., Dalsgaard A., Chai J. Y., Sohn W. M., Murrell K. D., 2007. Fishborne zoonotic intestinal trematodes, Vietnam. Emerg Infect Dis., 13: 1828–1833.

Dung B. T., Doanh P. N., The D. T. , Loan H. T., Losson B., Caron Y., 2013. Morphological and molecular characterization of Lymnaeid snails and their potential role in transmission of Fasciola spp. in Vietnam. Korean J Parasitol., 51: 657–662.

Frandsen F., Christensen N. O., 1984. An introductiontory guide to the identification of cercariae from African freshwater snails with special reference to cercariae of trematode species of medical and veterinary importance. Acta Trop.,

: 181–202.

Ginetsinskaya T. A., 1988. Trematodes, their life cycles, biology and evolution. Amerind Publishing Company, Pvt, NewDelhi (translation of the original Russian edition, 1968).

Hayes K. A., Joshi R. C., Thiengo S. C., Cowie R. H., 2008. Out of South America: multiple origins of non-native apple snails in Asia. Divers Distrib, 14: 701–712.

Hilbe J. M., 2008. Negative Binomial Regression. Cambridge University Press, Cambridge, UK. pp. 1–533.

Hung N. M., Duc N. V., Stauffer J. R., Madsen H., 2013. Use of black carp (Mylopharyngodon piceus) in biological control of intermediate host snails of fish–borne zoonotic trematodes in nursery ponds in the Red River Delta, Vietnam. Parasit Vectors., 6: 142.

Keiser J., Utzinger J., 2009. Food-borne trematodiases. Clin Microbiol Rev., 22: 466–483.

Kino H., Inaba H., Nguyen V. D., Le V. C., Dang T. S., Hoan T. H., Toan N. D., Le C. D., Sano M., 1998. Epidemiology of clonorchiasis in Ninh Binh province, Vietnam. Southeast Asian J Trop Med Public Health., 29: 250–254.

Le N. T., Doanh P. N., The D. T., 2000. Trematode larvae in the snail Parafossarulus striatulus (Bithyniidae) collected from Ba Vi and Ngoc Tao areas of Ha Tay. Academia Journal of Biology (Tap Chi Sinh hoc), 22: 15–21. (in Vietnamese).

Le N. T., The D. T., Ngai D. D., 1990. Examination of freshwater snails as intermediate hosts of trematodes, causing trematodosis in poultry”. In: Research work collection on Ecology and Biological Resources. Science and Technology Publishing House, Ha Noi, pp. 164–169. (in Vietnamese).

Lun Z. R., Gasser R. B., Lai D. H., Li A. X., Zhu X. Q., Yu X. B., Fang Y. Y., 2005. Clonorchiasis: a key foodborne zoonosis in China. Lancet Infect Dis., 5: 31–41.

Luu L. T., 2001. The VAC system in Northern Vietnam. In: Integrated agriculture–aquaculture: a primer. FAO Fisheries Technical Paper, pp. 149.

Madsen H., Bui T. D., Dang T. T., Nguyen V. K., Dalsgaard A., Phan T. V., 2015. The role of rice fields, fish ponds and water canals for transmission of fish-borne zoonotic trematodes in aquaculture ponds in Nam Dinh province, Vietnam. Parasit Vectors., 8: 625.

Madsen H., Coulibaly G., Furu P., 1987. Distribution of freshwater snails in the river Niger basin in Mali with special reference to the intermediate host of Schistosomes. Hydrobiol., 146: 77–88.

Madsen H., Hung N. M., 2014. An overview of freshwater snails in Asia with main focus on Vietnam. Acta Trop., 140: 105–117.

Mas-Coma S., Bargues M. D., 1997. Human liver flukes: a review. Res Rev Parasitol., 57: 145–218.

Nguyen M. H., 2016. Controlling trematodiases in Vietnam: actuality, challenges and trends for resolving these problems. Vietnam Science and Technology, 7: 11–18.

Nguyen M. H., Nguyen T. H., Pham T. L., 2018. Infection status of digenea larvae-cercariae in snails collected from aquaculture ponds in Northern provinces in Vietnam. Science and Technology Journal of Agriculture and Rural Development, 341: 78–84. (in Vietnamese).

Nguyen M. H., Do T. D., Nguyen T. L. A., Phan T. V., Bui N. T., Nguyen V. H., Hoang V. H., Le X. C., 2015. Current status of fish–borne zoonotic trematode infection in Gia Vien district, Ninh Binh province, Vietnam. Parasites & Vectors, 8: 21.

Nguyen M. H., Hoang V. H., Ho T. L., Tatonova Y. V., Madsen H., 2021. Are Melanoides tuberculata and Tarebia granifera (Gastropoda, Thiaridae), suitable first intermediate hosts of Clonorchis sinensis in Vietnam? PLOS Negl Trop Dis., 15(1): e0009093.

Nguyen M. H., Madsen H., Fried B., 2013. Global status of fish–borne zoonotic trematodiasis in humans. Acta Parasitol., 58: 231–258.

Nguyen T. L. A., Phuong T. N., Ha H. G., Luong T. T., Johansen M. V., Murrell K. D., Thamsborg S. M., 2008. Evaluation of techniques for detection of small trematode eggs in feces of domestic animals. Vet Parasitol.,156: 346–349.

Nguyen V. D., Murrell K. D., Cong L. D., Cam P. D., Chau L. V., Toan N. D., Dalsgaard A., 2003. The food-borne trematode zoonoses of Vietnam. Southeast Asian J Trop Med Public Health., 34: 12–34.

Phan T. V., Ersbøll A. K., Te B. Q., Hang N. T., Murrell K. D., Dalsgaard A., 2010a. Fish-borne zoonotic trematodes in cultured and wild-caught freshwater fish from the Red river delta, Vietnam. Vector Borne Zoonotic Dis., 10: 861–866.

Phan V. T., Ersbøll A. K., Nguyen V. K., Madsen H., Dalsgaard A., 2010b. Farm-level risk factors for fishborne zoonotic trematode infection in integrated small–scale fish farms in Northern Vietnam. PLoS Negl Trop Dis., 4: e742.

Schell S. C., 1985. Trematodes of North America (north of Mexico). University Press of Idaho, pp. 263.

Shan Lv., Guo Y. H., Nguyen M. H., Sinuon M., Sayasone S., et al., 2018. Invasive Pomacea snails as important intermediate hosts of Angiostrongylus cantonensis in Laos, Cambodia and Vietnam: Implications for outbreaks of eosinophilic meningitis. Acta Trop., 183: 32–35.

Thien P. C., Dalsgaard A., Nhan N. T., Olsen A., Murrell K. D., 2009. Prevalence of zoonotic trematode parasites in fish fry and juveniles in fish farms of the Mekong delta, Vietnam. Aquaculture, 295: 15.

Tucker C., Avery J., Engle C., Goodwin A., 2004. Industry profile: Pond–Raised Channel Catfish, A review developed for the National Risk Management Feasibility Program for Aquaculture. Missisippi State University, Agreement No RMA 01-IE-0831-127.

WHO, 1995. Control of foodborne trematode infection. Report of a WHO study group. WHO Technical Report Series, 849: 1–157.

Yu S. H., Mott K. E., 1994. Epidemiology and morbidity of Food-borne intestinal trematode infections”. In: Report of World Health Organization. Schistosomiasis Control Unit, Division of Control of Tropical Diseases. Geneva. WHO Library Cataloguing in Publication Data, pp. 1–26.




How to Cite

Manh Hung, N. (2022). Trematode larval infections in snails collected from aquaculture ponds in Ha Noi and Yen Bai, Vietnam. Academia Journal of Biology, 44(2), 43–52.