Efficiency assessment of microplastic extraction from green mussel \(\textit{Perna viridis}\) Linnaeus


  • Doan Thi Oanh Hanoi University of Natural Resources and Environment, Vietnam
  • Duong Thi Thuy Graduate University of Science and Technology, VAST, Vietnam
  • Nguyen Thi Nhu Huong Institute of Environmental Technology, VAST, Vietnam
  • Hoang Thi Quynh Institute of Environmental Technology, VAST, Vietnam
  • Vu Thi Nguyet Institute of Environmental Technology, VAST, Vietnam
  • Phuong Ngoc Nam Phu Tho College of Medicine and Pharmacy, Vietnam
  • Pham Quoc Tuan Phu Tho College of Medicine and Pharmacy, Vietnam
  • Ngo Thi Xuan Thinh Phu Tho College of Medicine and Pharmacy, Vietnam
  • Bui Huyen Thuong Graduate University of Science and Technology, VAST, Vietnam
  • Le Thi Phuong Quynh Institute of Natural Product Chemistry, VAST, Vietnam




Bivalves, digestion, microplastics, green mussel, Perna viridis


Microplastics with particle size less than 5 mm are becoming a raising global environmental crisis. These pollutants were found from the poles to the equator, in continental shelves, coasts and in the oceans, moreover, they have also been identified in the water columns, sediments and even in a variety of organisms. The majority of microplastics that ended up in the oceans originate from the land. Due to their small size, they are easily accumulated in the food chain, causing harmful effects on organisms and human health. The bivalves especially caught the interest of scientific researchers because of their direct contact with microplastics through the filter-feeding habit. Therefore, it is essential to develop methods to determine the presence of microplastics in these organisms and identify their source. This study evaluated the efficiency of extracting microplastics from the tissues of green mussels (Perna viridis) using KOH 10% solution to digest and KI 50% as the separating solution. Mussel soft tissue samples were spiked five different types of microplastics: polystyrene (PS), polyvinyl chloride (PVC), polyethylene terephthalate (PET), polypropylene (PP), high-density polyethylene (HDPE) and treated with KOH 10% solution and KI 50% solution. The presence of microplastics in some green mussel species was also investigated in some mussel farming areas in Giao Thuy, Nam Dinh province, Thi Nai, Quy Nhon, Binh Dinh province and Hue city, Thua Thien Hue province. The research results showed high efficiency of microplastic extraction and recovery with the range from 76% to 97%. Microplastic concentration obtained in all mussel samples variates from 1.0 ± 0.1 particles/g to 1.7 ± 0.6 particles/g, in which fiber microplastics predominated. Microplastics in mussel samples have small sizes of < 1,000 µm and 1,000–2,000 µm, make up 74.15–82.32% and 9.76–14.71%, respectively. Purple was dominant among all mussel samples. This study proved that using KOH 10% solution and KI 50% solution to isolate microplastics is a suitable approach and can be used in monitoring studies of microplastic pollution in bivalves.


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Alessio G., Pierluigi S., Kjell B. Ø., Gianna F., 2019. First occurrence and composition assessment of microplastics in native mussels collected from coastal and offshore areas of the northern and central Adriatic Sea. Environmental Science and Pollution Research, 26: 24407–24416.

Bråte I. L. N., Hurley R., Iversen K., Beyer J., Thomas K. V., Steindal C. C., Green N. W., Olsen M., Lusher A., 2018. Mytilus spp. as sentinels for monitoring microplastic pollution in Norwegian coastal waters: A qualitative and quantitative study. Environmental Pollution, 243 (A): 383–393.

Barrows A. P. W., Cathey S. E., Petersen C. W., 2018. Marine environment microfiber contamination: Global patterns and the diversity of microparticle origins. Environmental Pollution, 237: 275–284.

Claessens M., Van C. L., Vandegehuchte M. B., Janssen C. R., 2013. New techniques for the detection of microplastics in sediments and field collected organisms. Marine Pollution Bulletin, 70 (1–2): 227–233.

Conrad S., 2020. Microplastics in Mussels Along the Coast of Cape Town, South Africa. Bulletin of Environmental Contamination and Toxicology. https://doi.org/10.1007/s00128-020-028 09-w

Dehaut A., Cassone A. L, Frère L., Hermabessiere L., Himber C., Rinnert E., Rivière G., Lambert C., Soudant P., Huvet A., Duflos G., Paul-Pont I., 2016. Microplastics in seafood: Benchmark protocol for their extraction and characterization. Environmental Pollution, 215: 223–233.

Digka N., Catherine T., Michele T., Aikaterini A., Christina Z., 2018. Microplastics in mussels and fish from the Northern Ionian Sea. Marine Pollution Bulletin, 135: 30–40.

Duncan E. M., Broderick A. C., Fuller W. J., Galloway T. S., Godfrey M. H., Hamann M., Limpus C. J., Lindeque P. K., Mayes A. G., Omeyer L. C. M., Santillo D., Snape R. T. E., Godley B. J., 2019. Microplastic ingestion ubiquitous in marine turtles. Global Change Biology, 25: 744–752.

Herrera A., Garrido-Amador P., Martínez I., María D. S., López-Martínez J., Gómez M., Packard T. T., 2018. Novel methodology to isolate microplastics from vegetal-rich samples. Marine Pollution Bulletin, 129: 61–69.

ICES, 2015. OSPAR Request on Development of a Common Monitoring Protocol for Plastic Particles in Fish Stomachs and Selected Shell fish on the Basis of Existing Fish Disease Surveys.

Karami A., Abolfazl G., Cheng K. C., Nicholas R., Yu B. H., Babak S., 2016. A high-performance protocol for extraction of microplastics in fish. Science of the Total Environment, 578 (1): 485–494.

Lahens L., Strady E., Kieu-Le T. C., Dris R., Boukerma K., Rinnert E., Gasperi J., Tassin B., 2018. Macroplastic and microplastic contamination assessment of a tropical river (Saigon River, Vietnam) transversed by a developing megacity. Environmental Pollution, 236 : 661–671.

Li J., Qu X., Su L., Zhang W., Yang D., Kolandhasamy P., Li D., Shi H., 2016. Microplastics in mussels along the coastal waters of China. Environmental Pollution, 214: 177–184.

Li J., Lusher A. L., Rotchell J. M., Deudero S., Turra A., Bråte I. L. N., Shi H., 2019. Using mussel as a global bioindicator of coastal microplastic pollution. Environmental Pollution, 244: 522–533.

Mathalon A., Hill P., 2014. Microplastic fibers in the intertidal ecosystem surrounding Halifax Harbor, Nova Scotia. Marine Pollution Bulletin, 81: 69–79.

Mizraji R., Ahrendt C., Perez-Venegas D., Vargas J., Pulgar J., Aldana M., Patricio Ojeda F., Duarte C., Galbán-Malagón C., 2017. Is the feeding type related with the content of microplastics in intertidal fish gut? Marine Pollution Bulletin, 116: 498–500.

Nam N. P., Zalouk-Vergnoux A., Abderrahmane K., Catherine M., Frederic A., Laurence P., Fabienne L., 2017. Quantification and characterization of microplastics in blue mussels (Mytilus edulis): protocol setup and preliminary data on the contamination of the French Atlantic coast. Environmental Science and Pollution Research, 25 (7): 6135–6144.

Qu X., Su L., Li H., Liang M., Shi H., 2018. Assessing the relationship between the abundance and properties of microplastics in water and in mussels. The Science of the Total Environment, 621: 679–686.

Quinn B., Murphy F., Ewins C., 2017. Validation of density separation for the rapid recovery of microplastics from sediment, Analytical Methods, 9 (9): 1491–1498.

Renzi M., Guerranti C., Blaskovic A., 2018. Microplastic contents from maricultured and natural mussels. Marine Pollution Bulletin, 131: 248–251.

Rochman C. M., Tahir A., Williams S. L., Baxa D. V., Lam R., Miller J. T., Teh F., Werorilangi S., Teh S. J, 2015. Anthropogenic debris in seafood: Plastic debris and fibers from textiles in fish and bivalves sold for human consumption. Scientific Reports, 5:14340, 1–10.

Rocío S. P., Fernando S., Nora G., 2020. First record of microplastics in the mussel Limnoperna fortunei. Regional Studies in Marine Science, 38: 101360. doi: 10.1016/j.rsma.2020.101360

Su L., Xue Y., Li L., Yang D., Kolandhasamy P., Li D., Shi H., 2016. Microplastics in Taihu Lake, China. Environmental Pollution, 216: 711–719.

Teuten E. L., Saquing J. M., Knappe D. R. U., Barlaz M. A., Jonsson S., Bjorn A., Rowland S. J., Thompson R. C., Galloway T. S., Yamashita R., Ochi D., Watanuki Y., Moore C., Pham H. V., Tana, T. S., Prudente M., Boonyatumanond, R., Zakaria M. P., Akkhavong K., Ogata Y., Hirai H., Iwasa S., Mizukawa K., Hagino Y., Imamura A., Saha M., Takada H., 2009. Transport and release of chemicals from plastics to the environment and to wildlife. Philosophical Transactions of The Royal Society B Biological Sciences, 364 (1526): 2027–2045.

Thielea C. J., Malcolm D. H., Andrea E. R., 2019. Evaluation of existing methods to extract microplastics from bivalve tissue: Adapted KOH digestion protocol improves filtration at single-digit pore Size. Marine Pollution Bulletin, 142: 384–393.

Vandermeersch G., Van C. L., Janssen C. R., Marques A., Granby K., Fait G., Kotterman M. J. J, Diogène J., Bekaert K., Robbens J., Devriese L., 2015. A critical view on microplastic quantification in aquatic organisms. Environmental Research, 143: 46–55.

Van C. L., Janssen C. R., 2014. Microplastics in bivalves cultured for human consumption. Environmental Pollution, 193: 65–70.

Von M. N., Burkhardt-Holm P., Köhler A., 2012. Uptake and effects of microplastics on cells and tissue of the blue mussel Mytilus edulis L. after an experimental exposure. Environmental Science and Technology, 46: 11327–11335.

De Witte B., Devriese L., Bekaert K., Hoffman S., Vandermeersch G., Cooreman K., Robbens J., 2014. Quality assessment of the blue mussel (Mytilus edulis): comparison between commercial and wild types. Marine Pollution Bulletin, 85: 146–155.

Woods M. N., Stacka M. E., Fields D. M., Shawa S. D., Matraib P. A., 2018. Microplastic fiber uptake, ingestion, and egestion rates in the blue mussel (Mytilus edulis). Marine Pollution Bulletin, 137: 638–645.




How to Cite

Oanh, D. T., Thuy, D. T., Huong, N. T. N. ., Quynh, H. T., Nguyet, V. T., Nam, P. N., Tuan, P. Q., Thinh, N. T. X., Thuong, B. H., & Quynh, L. T. P. (2021). Efficiency assessment of microplastic extraction from green mussel \(\textit{Perna viridis}\) Linnaeus. Academia Journal of Biology, 43(4), 55–66. https://doi.org/10.15625/2615-9023/16153




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