Effects of <i> in vitro </i> plant ages on the subsequent growth of <i> Plumbago indica </i> l. after <i> ex vitro </i> transplantation
Keywords:plumbago indica, ex vitro, in vitro, plant propogation, survival rate
The Indian leadwort (Plumbago indica L.) of the family Plumbaginaceae is a plant with high pharmaceutical value, as it contains plumbagin, a naphthoquinone with antibacterial, antifungal and anticancer properties. Among the propagation methods for the Indian leadwort, in vitro propagation is considered an effective method in producing disease-free transplants in a short period of time with high propagation rate. When plants grown in vitro are transferred to ex vitro condition, the environmental factors in the nursery house such as light, temperature, humidity and microorganism in the soil will affect their growth. Characteristics of transplants themselves is also critical for the subsequent growth. It is, thus, essential to establish the standards to evaluate and qualify in vitro plants for transplanting to ex vitro condition. Among these standards, the culture age of in vitro plants affects the maturations of their root, stem and leaves, which can in turn influence the acclimating ability and growth of in vitro plants after transplantation. The purpose of this study is to investigate the effects of the culture age of in vitro Indian leadwort plants on their performance during ex vitro stage. For this purpose, three different culture ages of uniform in vitro plants, 35, 42 and 49 day-old, were studied. After 28 days of cultivation in the nursery house under the light intensity of 70 ± 10 µmol m-2 s-1, temperature of 35 ± 4 oC and relative humidity (RH) of 60 ± 10%, all three treatments achieved 100% survival rate. Increased fresh and dry weights and percentage of dry matter after cultivation in ex vitro condition were not statistically different between 42 day-old and 49 day-old in vitro plants, but were significantly different between these plants and 35 day-old in vitro plants. The development of shoot and root in ex vitro stage of 42 day-old and 49 day-old in vitro plants was more balanced, as shown by the higher ratio of shoot/root dry weight, than 35 day-old in vitro plants. The results of this study showed that for this Plumbago species, bigger in vitro plants led to better growth during ex vitro stage. These results also indicated that it was possible to transfer in vitro Plumbago plants to ex vitro condition after 5 weeks of in vitro culture stage.
Agren G. I., Ingestad T., 1987. Root:shoot ratio as a balance between nitrogen productivity and photosynthesis. Plant Cell Environ. 10: 579–586. https://doi.org/ 10.1111/1365-3040.ep11604105
Bhadra S. K., Akhter T., Hossain M. M., 2009. In vitro micropropagation of Plumbago indica L. through induction of direct and indirect organogenesis. Plant Tissue Cult. & Biotech. 19(2): 169 – 175. https://doi.org/10.3329/ptcb.v19i2.5434
Bolar J. P., Norelli J., Aldwinckle H.S., Hanke V., 1998. An efficient method for rooting and acclimatization of micropropagated apple culture. Hort. Sci. 37: 1241–1252. https://doi.org/10.21273/ HORTSCI.33.7.1251
Bonifas K. D., Lindquist J. L., 2006. Predicting biomass partitioning to root versus shoot in corn and velvetleaf (Abutilon theophrasti). Weed Sci., 54: 133–137. https://doi.org/10.1614/WS-05-079R1.1
Chandra S., Bandopadhyay R., Kumar V., Chandra R., 2010. Acclimitization of tissue cultured plantlets: from laboratory to land. Biotech. Lett., 32: 1199–1205. https://doi.org/10.1007/s10529-010-0290-0
Chen J-L., Reynolds J. F., Harley P. C., Tenhunen J. D., 1993. Coordination theory of leaf nitrogen distribution in a canopy. Oecologia., 93: 63–69. https://doi.org/10.1007/BF00321192
Deb C. R, Imchen T., 2010. An efficient in vitro hardening of tissue culture raised plants. Biotechnology, 9: 79–83. https://doi.org/10.3923/biotech.2010.79.83
Donnelly D. J., Vidaver W. E., Lee K. Y., 1985. The anatomy of tissue cultured red raspberry prior to and after transfer to soil. Plant Cell Tiss. Org. Cult., 4: 43 – 50. https://doi.org/10.1007/BF00041654
Do B. H., Dang C. Q., Bui C. X., Nguyen D. T., Do D. T., Pham H. V., Vu L. N., Pham M. D., Pham M. K., Doan N. T., Nguyen T., Tran T., 2004. Chapter 31: Plumbago zeylanica L. in medicinal plants and animals in Vietnam. Science and Technology Publisher, Hanoi, Vietnam. Vol. 1, pp. 148 – 150 (in Vietnamese).
Ericsson T., 1996, Growth and shoot: root ratio of seedlings in relation to nutrient availability. Plant and Soil, 168–169: 205–214. https://doi.org/10.1007/978-94-011-0455-5_23
Galal A M., Raman V., Avula B., Wang Y H., Rumalla C S., Weerasooriya A D., Khan I A., 2013. Comparative study of three Plumbago L. species (Plumbaginaceae) by microscopy, UPLC-UV and HPTLC. J. Nat. Med., 67(3): 554–561. https://doi.org/ 10.1007/s11418-012-0717-0
Hilbert D. W., 1990. Optimization of plant root: shoot ratios and internal nitrogen concentration. Ann. Bot. 66: 91–99. https://doi.org/10.1093/oxfordjournals.aob
Hoffmann W. A., Poorter H. IK., 2002. Avoiding bias in calculations of relative growth rate. Ann. Bot., 80: 37–42. https://doi.org/10.1093/aob/mcf140
Jamal M. S., Parveen S., Beg M. A., Suhail M., Chaudhary A G A., Damanhouri G A., Abuzenadah A M., Rehan M., 2014. Anticancer compound Plumbagin and its molecular targets: A structural insight into the inhibitory mechanisms using computational approaches. Plos One. 9(2): 1–12. https://doi.org/10.1371/journal. pone.0087309
Jose B., Dhanya B P., Silja P. K., Krishnan P. N., Satheeshkumar K., 2014. Plumbago rosea L. - A review on tissue culture and pharmacological research. Int. J. Pharm. Sci. Rev. Res., 25(1): 246–256.
Levin S. A., Mooney H. A., Field C., 1989. The dependence of plant root : shoot ratios on internal nitrogen concentration. Ann. Bot., 64: 71–75. https://doi.org/10.1093/ oxfordjournals.aob.a087810
Li Y.Y., Lü X.T., Wang Z. W., Zhou C., Han X. G., 2014. Linking relative growth rates to biomass allocation: the responses of the grass Leymus chinensis to nitrogen addition. Phyton., 83: 283–289. https://doi.org/10.32604/phyton.2014.83.283
Lindquist J. L., 2001. Light-saturated CO2 assimilation rates of corn and velvetleaf in response to leaf nitrogen and development stage. Weed Sci., 49: 706–10. https://doi.org/10.1614/0043-1745(2001)0 49[0706:LSCARO]2.0.CO;2
Mallavadhani U V., Sahu G., Muralidhar J., 2002. Screening of Plumbago species for the bio-active marker plumbagin. Pharm. Biol., 40(7): 508–511. https://doi.org/ 10.1076/phbi.40.7.508.14685
Marcelis L. F. M., 1996. Sink strength as a determinant of dry matter partitioning in the whole plant. J. Exp. Bot., 47:
Morel G., Wetmore R. H., 1951. Tissue culture of monocotyledons. Am. J. Bot., 38(2): 138–140. https://doi.org/ 10.2307/2437836
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant, 15(3): 473–497. https://doi.org/ 10.1111/j.13993054.1962.tb08052.x
Nguyen S. D., Phan H. N., 2013. Chapter 5: Extracted and identified active substances of plant growth regulators by bioassays in internship in plant physiology. Publishing House of HCM National, HCM City, Vietnam, pp. 57–66 (in Vietnamese).
Nguyen Q. T., Xiao Y, Kozai T., 2016. Photoautotrophic micropropagation. pp. 271-283. In: Kozai T., Niu G., Takagaki M. (eds.) Plant Factory - An indoor vertical farming system for efficient quality food production (1st edition). Academic Press, Elsevier. California, USA. (ISBN: 978-0-12-801775-3). http://doi.org/10.1016/B978-0-12-801775-3.00020-2
Panichayupakaranant P., Tewtrakul S., 2002. Plumbagin production by root cultures of Plumbago rosea. Electron. J. Biotech., 5(3): 228–232. https://doi.org/10.2225/ vol5-issue3-fulltext-4
Pant M., Lal A., Rana S., Rani A., 2012. Plumbago zeylanica L.: a Mini Review. Int. J. Pharm. Appl., 3(3): 399–405.
Pospíšilová J., Tichá I., Ek P K Č., Haisel D., 2007. Acclimation of plantlets to ex vitro conditions: Effects of air humidity, irradiance, CO2 concentration and abscisic acid (a review). Acta Hort., 42(96): 481–497. https://doi.org/10.17660/ActaHortic. 2007.748.2
Priyanjani S. A., Senarath W. TPSK., 2019. Propagation of Plumbago indica L. (Plumbaginaceae) through direct organogenesis and induction of callus. Int. J. Bot. Studies. 4(5): 4–7
Sumathy N., Sanjayan K.P., 2011. Effect of Plumbagin, a Napthoquinone of plant origin, on the consumption and post ingestional physiological parameters of food utilization in Spodoptera litura (Fab) (Lepidoptera: Noctuidae). GJAR, 1(2): 83–88.
Weraduwage S. M., Chen J., Anozie F. C., Morales A., Weise S. E., Sharkey T. D., 2015. The relationship between leaf area growth and biomass accumulation in Arabidopsis thaliana, Front. Plant Sci, 6: 167. https://doi.org/10.3389/fpls.2015. 00167
Zakaria N. Y., Ismail M. R., Awang Y., Wahab P. E. M., Berahim Z., 2020. Effect of root restriction on the growth, photosynthesis rate, and source and sink relationship of chili (Capsicum annuum L.) grown in soilless culture. BioMed. Res. Int., Vol. 2020: 1–14. https://doi.org/ 10.1155/2020/2706937