The DNA barcodes for the species delimitation of the genus \(\textit{Tylopus}\) Jeekel, 1968 in Vietnam (Diplopoda: Polydesmida: Paradoxosomatidae)

Nguyen Duc Anh; Nguyen Thi Thu Anh; Phung Thi Hong Luong; Dang Thi Hoa; Nguyen Giang Son

doi:10.15625/2615-9023/15761

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Authors

Nguyen Duc Anh Institute of Ecology and Biological Resources, VAST, Vietnam https://orcid.org/0000-0001-9273-0040
Nguyen Thi Thu Anh Institute of Ecology and Biological Resources, VAST, Vietnam
Phung Thi Hong Luong Institute of Ecology and Biological Resources, VAST, Vietnam
Dang Thi Hoa Institute of Ecology and Biological Resources, VAST, Vietnam
Nguyen Giang Son Institute of Ecology and Biological Resources, VAST, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/15761

Keywords:

Molecular taxonomy, biodiversity, millipedes, COI gene.

Abstract

The 680 bp fragment of the COI gene was employed for DNA barcodes of the millipede genus Tylopus in Vietnam. A total of 22 samples representing for 14 morphological Tylopus species were analyzed. The K2P genetic divergence between Tylopus species ranges from 12.2% to 18.9% with a mean of 15 ± 1%. The intraspecific divergences are slightly different between species, from 3% to 5%. The AGBD analysis and phylogenetic trees also support 14 morphological species. It is also suggested to have more COI sequences of more species for better barcode reference library and better molecular species identification.

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