Genetic characteristics of <i>Panax vietnamensis </i>Ha & Grushv.  populations based on ssr

Nguyen Thi Hong Mai; Le Thanh Son; Nguyen Thi Phuong Trang

doi:10.15625/0866-7160/v42n1.13906

Author affiliations

Authors

Nguyen Thi Hong Mai
Le Thanh Son
Nguyen Thi Phuong Trang

DOI:

https://doi.org/10.15625/0866-7160/v42n1.13906

Keywords:

Panax vietnamensis, conservation, ginseng, microsatellite.

Abstract

The results of genetic analysis of two Panax vietnamensis populations, collected in Tac-ngo ginseng garden, Tra Linh commune, Quang Nam Province and Mang ri ginseng garden, Kon Tum Province, showed that the population of ngoc linh ginseng in Quang Nam has higher alen frequency compared to the Ngoc Linh ginseng population in Kon Tum (AR = 2.27 > AR = 2.05). The population of ngoc linh ginseng in Quang Nam has the expected heterozygous frequency (He) higher than the expected heterozygous frequency of the ginseng population in Kon Tum (0.42 > 0.4). The observed heterozygous frequency (Ho) of the ngoc linh population in Quang Nam was also higher than the observed heterozygous frequency compared to the ngoc linh ginseng population in Kon Tum (0.49 > 0.43). These results demonstrated that both populations of ngoc linh ginseng in Quang Nam and Kon Tum are maintaining high heterozygous frequencies; self-pollination has not affected to the genetic structure of these both populations yet. However, the population of ngoc linh ginseng in Quang Nam showed a higher level of diversity and heterozygous frequency than the ngoc linh ginseng population in Kon Tum.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Choi I. J., Kim H., Kim N. H., Choi B. S., Ahn I. O., Lee J. S., and Yang T. K., 2011. Development of reproducible EST-derived SSR markers and assessment of genetic diversity in Panax ginseng cultivars and related species. Journal of Ginseng reseach, 35: 399−412.

Do Tat Loi, 2004, Vietnamese medicinal plants and herbs. Medicine Publishing House, pp. 808−810. (In Vietnamese)

Doyle J. J., Doyle J. L., 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 13−15.

Elena A. V., Iury Yu. A., Reunova G. D., T. P. T. Nguyen, Yuri N. Z., 2018. A comparative Analysis of genetic Variability and differentiation in Panax vietnamensis Ha et Grushv. and P. gienseng C.A. Meyer using ISSR markers. Russian journal of genetics, 54(2): 262−265.

Galina D. Reunova, Irina L. Kats, Tamara I. Muzarok, Trang N. T. P, The Dang Tat, Yuri N. Zhuravlev, 2010. Genetic diversity of Panax ginseng C. A. Meyer, inferred from amplified fragment length polymorphism markers”.

Ha T. D. and Grushvitzky I. V., 1985. New species in Panax (Araliaceae) in Viet Nam. J. Botany, 70: 518–522.

Keller L. F. and Waller D. M., 2002. Inbreeding effects in wild populations. Trends in ecology & evolution, 17(5), 230–241.

Kim J., Jo B. H., Lee K. L., Yoon E. S., Ryu G. H., Chung K. W., 2007. Identification of new microsatellite markers in Panax ginseng. Molecules and Cell, 24: 60−68.

Lahaye R., van der Bank M., Bogarin D., Warner J., Pupulin F., Gigot G., Maurin O., Dathoit S., Barraclough T. G., Savolainen V., 2008. DNA barcoding the floras of biodiversity hotspots. PNAS, 105(8): 2923−2928.

Le Ngoc Trieu, Nong Van Duy, Vu Tien Chinh, Tran van Tien, 2016a. Genetic diversity of Panax vietnamensis var. fucidicus K. Komatsu, S. Zhu & s.Q.cai population in western north of Vietnam detected by Inter simple sequence repeat markers. Vietnam Journal of Biotechnology, 14(4): 619−627 (In Vietnamese with English summary).

Le Ngoc Trieu, Nguyen Tuong Mien, Tran Van Tien, Nguyen Van Ket, Nong Van Duy 2016b. Genetic diversity of Panax

stipuleanatus Tsai in North Vietnam detected by inter simple sequence repeat (ISSR) markers. Biodiversity and Ecosystems, pp. 506−511.

Le Thanh Son and Nguyen Tap, 2006. Ecological characteristics of Panax vietnamensis Ha et Grushv. Journal of Medicinal Materials, 14(4): 145−147 (In Vietnamese with English summary).

MOST (Ministry of Science and Technology) and VAST (Vietnam Academy of Science and Technology), 2007. Vietnam red data book, part II. Plants (In Vietnamese)

Nguyen Tien Ban, 2003. List of Vietnamese Plant species, Agriculture Publishing House, Ha Noi. Part 2, p. 1078−1079. (In Vietnamese).

Rox Peakall, Peter E. Smouse, 2012. GenAlex 6.5: Genetic analysis in Excel Population genetic software for teaching and research-an update. Bioinformatics, 28(19): 2537−2539.