Investigating the frequency of alleles of crtRB1 and LcyE genes that are favoable for -carotene accumulation in some maize lines cultivated in VietNam
Author affiliations
DOI:
https://doi.org/10.15625/0866-7160/v40n2.13239Keywords:
Allele frequency, b-carotene, favorable alleles, maize (Zea mays L.) pro-vitamin A carotenoid.Abstract
Provitamin A carotenoid content, including b-carotene in corn kernels is very low and varies widely in maize lines /varieties. Results of the study on the role of the crtRB1 and LcyE gene alleles in the accumulation of b-carotene in maize showed that some alleles could regulate the increase in b-carotene content. These alleles are called favorable alleles as they are favorable for b-carotene accumulation. However, the frequency of these alleles in traditional maize varieties is very low. And so they are known as rare alleles. The identification of favorable alleles and high b-carotene maize lines for maize improvement in the direction of increased provitamin A carotenoid content, especially b-carotene, is very important. In this study, we investigated the frequency of alleles of the crtRB1 and LcyE genes that are favorable for β-carotene accumulation in 20 maize lines cultivated in Vietnam. The results showed that the favorable allele frequency of crtRB1 in the studied maize lines was 25% and that of LcyE was 5%. Four lines (H240, H95, H20 and Ngô nâu vàng Lắk, Đắk Lắk) carry favorable alleles at 3’ end of crtRB1 gene and have high b-carotene content (from 6,100 to 17,247 mg/g). These maize lines are an important source for researches in the improvement of b-carotene content in maize.
Downloads
Metrics
References
Burri BJ (1997) Beta-carotene and human health: a review of current research. Nutrition Research 17: 547-5
Azmach G, Gedil M , Menkir A, Spillane C (2013) Marker-trait association analysis of functional gene markers for provitamin A levels across diverse tropical yellow maize inbred lines. BMC Plant Biology 2013 13:227
Babu R, Rojas NP, Gao S, Yan J, Pixley K (2013) Validation of the effects of molecular marker polymorphisms in LcyE and crtRB1 on provitamin A concentrations for 26 tropical maize populations. Theor Appl Genet 126: 389–399 DOI 10.1007/s00122-012-1987-3
Harjes C E, Rocheford TR, Bai L, Brutnell T P, Kandianis C B, Sowinski SG, Buckler E S (2008) Natural genetic variation in lycopene epsilon cyclase tapped for maize biofortification. Science 319: 330–333
HarvestPlus 2007. The biofortification challenge programme. Annual report, HarvestPlus program. Washington DC
Kurilich AC, Juvik JA (1999) Quantification of carotenoid and tocopherol antioxidants in Zea mays. J Agric Food Chem 47:1948–55
Muthusamy V, Hossain F, Thirunavukkarasu N, Saha S, Gupta1 HS (2015) Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme. Cogent Food & Agriculture 1: 1033141, http://dx.doi.org/10.1080/23311932.2015.1033141
Pixley K, Babu R, Yan J, Palacios-Rojas N (2010) Challenges, progress, and state of breeding for pro-vitamin A. First Global Conference on Biofortification: From Discovery to Delivery, 9–11 Nov. 2010. Washington, DC
Rivera S, Canela R, 2012. Influence of sample processing on the analysis of carotenoids in maize. Molecules 17: 11255-11268
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81: 8014-8018.
Selvi TD, Senthil N , Yuvaraj A , John Joel A , Mahalingam A, Nagarajan P, Vellaikumar S , Srimathi P , Raveendran M, Nepolean T (2014) Assessment of crtRB1 Polymorphism Associated with Increased β-Carotene Content in Maize (Zea mays L.) Seeds, Food Biotechnology 28: 41-49, DOI: 10.1080/08905436.2013.870077
Tanumihardjo SA, Russel RM, Stephensen CB, Gannon BM, Craft NE, Haskell MJ, Lietz G, Schulze K, Raiten DJ (2016) Biomarkers of nutrition for development (BOND) – vitamin A review. J Nutr 146:1816S–1848S.
US Department of Agriculture (USDA) (2007) USDA Table of Nutrient Retention Factors. Release 6. United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, USA
US Department of Agriculture (USDA) (2010) A new approach that saves eyesight and lives in the developing world https://www.ars.usda.gov/
World Health Organization (2010) World health statistics. Geneva, Switzerland: WHO
Yan J, Bermudez-Kandianis CB, Harjes CE, Bai L, Kim E, Yang X, Skinner D, Fu Z, Mitchell S, Li Q, Salas-Fernandez M, Zaharieva M, Babu R, Fu Y, Palacios N, Li J, DellaPenna D, Brutnell T, Buckler E, Warburton M, Rocherford T (2010) Rare genetic variation at Zea mays crtRB1 increases -carotene in maize grain. Nat Genet 42: 322–327
Zhang X, Pfeiffer WH, Palacios-Rojas N, Babu R, Bouis H, et al. (2012) Probability of success of breeding strategies for improving provitamin A content in maize. Theor Appl Genet 125: 235–246
Zunjare RU, Hossain F, Muthusamy V, Baveja A, Chauhan HS, Thirunavukkarasu N, Saha S, Gupta HS (2017) Influence of rare alleles of β-carotene hydroxylase and lycopene epsilon cyclase genes on accumulation of provitamin A carotenoids in maize kernels. Plant Breeding 136(6): 872-880
Zunjare RU, Chhabra R, Hossain F, Baveja A, Muthusamy V, Gupta HS (2018) Molecular characterization of 5′ UTR of the lycopene epsilon cyclase (LcyE) gene among exotic and indigenous inbreds for its utilization in maize biofortification. 3. Biotech 8: 75, https://doi.org/10.1007/s13205-018-1100-
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 TAP CHI SINH HOC
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
