Nghiên cứu điều chế chế phẩm chống oxi hóa từ rễ thổ phục linh (Simila glabra Roxb.) của Việt Nam

Abstract

An antioxidant functional food from the root of Smilax glabra Roxb. of Vietnam (TPL-As40) was prepared, by using the purified EtOH extract which contains high astilbin content (46.1 %) as determined by HPLC analysis. The antioxidant activity of this functional food was evaluated by different methods: DPPH assay for free radical scav enging capacity, MDA assay for inhibition of lipid peroxidation and MTT assay for hepato protective effection H₂O₂-injured mouse hepatocytes. Accordingly, the TPL-As40 had significant antioxidant property with SC₅₀ value of 19.8 µg/ml (DPPH assay), IC₅₀ value of 9.32 µg/ml (MDA assay) and EC₅₀ value of 20.95 µg/ml (MTT assay). The toxicity evaluation in mice indicated that theTPL-As40 product was safe for use as functional food according to OECD.