Immunomodulatory and antioxidant activities of the two new neo-cleodane obtained from the Vietnamese Ban-chi-lien plant (Scutellaria barbata D.don)

Abstract

Scutellaria barbata D.don is a medicinal herb that is widely distributed in Vietnam. In the traditional medicine, this plant is efficient in treating cancer patients. Thus, the plant gained much attention of researchers. Recently, the two novel neo-cleodane compounds which are scutebata S and scutebata T has been isolated from Scutellaria barabata D.don of Vietnam (Thao et al., 2014). These compounds showed fair cytotoxic activity on different tested cancer cell lines. In this study, the antioxidant activities and immunomodulating properties of scutebata S and T were evaluated. The results indicated that both scutebata S and scutebata T reduced the lipid peroxidation and protected liver cells against H2O2. However scutebata T possessed stronger activities than those of scutebata S. At the concentration of 100 µg/ml, scutebata T resulted 16.07% inhibitory effect of lipid peroxidation and maintained the hepatocyte viability up to 76.21% in compared with H2O2 alone (54,74%). The immunomodulating properties of these compounds were assessed throught the proliferation of lymphocytes and macrophage cellular lysosomal enzyme activity. Scutebata S inhibited the proliferation of lymphocytes at all study concentrations. Whereas, scutebata T stimulated lymphocyte proliferation and enhanced cellular lysosomal enzyme activity of macrophages at concentrations in the range of 0.08 µg/ml/ml to 20 µg/ml. Therefore, scutebata S and T demonstrated their positively immunomodulated activities at in vitro level.