Genetic diversity of scorpions in Thien Duong and Tien Son caves base on mitochondrial COI gene analysis

Van Tang Duong; Thi Viet Thanh Tran; Van Mien Nguyen; Thuy Vi Phan; Thi Hang Tran; Dinh Sac Pham

doi:10.15625/vjbt-22437

Author affiliations

Authors

Van Tang Duong \(^1\) Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Thi Viet Thanh Tran \(^1\) Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Van Mien Nguyen \(^1\) Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Thuy Vi Phan \(^1\) Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam
Thi Hang Tran \(^2\) Centre for Natural Resources and Environmental, Viet Nam National University, 19 Le Thanh Tong, Hoan Kiem, Ha Noi, Vietnam
Dinh Sac Pham \(^1\) Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Hanoi, Vietnam https://orcid.org/0000-0001-8594-5270

DOI:

https://doi.org/10.15625/vjbt-22437

Keywords:

COI gene, evolutionary analysis, genetic diversity, scorpion, Vietbocap

Abstract

The mitochondrial cytochrome c oxidase subunit I (COI) genes of scorpions Vietbocap from the Thien Duong and Tien Son caves were sequenced and compared to assess the genetic diversity of populations. Phylogenetic trees and evolutionary maps were constructed to reveal the genetic affinities among the populations. A total of 32 polymorphic sites were identified in the 628-bp sequence of the mtDNA COI gene following sequence correction. The polymorphic loci were more than 10, the haplotype diversity was > 0.5, and the nucleotide diversity (Pi) was > 0.005. A total of five haplotypes were detected based on nucleotide variation among sequences. Scorpions in both Thien Duong and Tien Son caves were divided into three groups with significant genetic differences. The group in Tien Son cave exhibited clear distinctions when compared to the two groups in Thien Duong cave, with genetic distances of 4.33% and 4.64%, respectively. The two groups in Thien Duong cave were less differentiated, with a genetic distance of 0.68%. These results provide important genetic information for taxonomic study and offer a scientific basis for the existence of more than one species of scorpion within the populations at Thien Duong and Tien Son caves.

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References

Arantes E.C., Prado W.A., Sampaio S.V. and Giglio J.R. (1989). A simplified procedure for the fractionation of Tityus serrulatus venom: isolation and partial characterization of TsTX-IV, a new neurotoxin. Toxicon 27(8), 907-916. https://doi.org/10.1016/0041-0101(89)90102-5

Avise J. C. (2000). Phylogeography: The history and formation of species. Harvard University Press. https://doi.org/10.2307/j.ctv1nzfgj7

Avise J. C. (2004). Molecular markers, natural history, and evolution (2nd edition). Sinauer Associates. https://doi.org/10.1093/auk/121.4.1298

Bandelt H., Forster P. and Röhl A. (1999). Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16(1), 37–48.

https://doi.org/10.1093/oxfordjournals.molbev.a026036

Catterall W.A., Cestele S., Yarov-Yarovoy V., Yu F.H., Konoki K. and Scheuer T. (2007). Voltage-gated ion channels and gating modifier toxins, Toxicon, 49(2), 124-141.

https://doi.org/10.1016/j.toxicon.2006.09.022

Cohen L., Lipstein N., Karbat I., Ilan N., Gilles N., Kahn R. and et al. (2008). Miniaturization of scorpion beta-toxins uncovers a putative ancestral surface of interaction with voltage-gated sodium channels. Journal of Biological Chemistry, 283(22), 15169-15176.

https://doi.org/10.1074/jbc.m801229200

DeBin J.A., Maggio J.E. and Strichartz G.R. (1993). Purification and characterization of chlorotoxin, a chloride channel ligand from the venom of the scorpion. American Journal of Physiology, 264, C361-C369.

https://doi.org/10.1152/ajpcell.1993.264.2.c361

Dehesa-Davila M. and Possani L.D. (1994). Scorpionism and serotherapy in Mexico. Toxicon, 32(9), 1015-1018.

https://doi.org/10.1016/0041-0101(94)90383-2

DNA Sequence Assembler v4 (2013). SciVance Technologies, www.DnaBaser.com

Excoffier L. and Lischer H.E.L. (2010). Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10, 564-567.

https://doi.org/10.1111/j.1755-0998.2010.02847.x

Folmer O., Black M., Hoeh W., Lutz R. and Vrijenhoek R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5), 294–299.

https://doi.org/10.1371/journal.pone.0013102

Funk D.J. and Omland K. E. (2003). Species-level paraphyly and polyphyly: Frequency, causes, and consequences, with insights from animal mitochondrial DNA. Annual Review of Ecology, Evolution, and Systematics, 34, 397–423. https://doi.org/10.1146/annurev.ecolsys.34.011802.132421

Gulewitsch W. and Amiradžibi S. (1900). Ueber das Carnosin, eineneueorganische Base des Fleischextractes. Berichte der deutschen chemischen Gesellschaft, 33(2), 1902-1903.

https://doi.org/10.1002/cber.19000330275

Hebert P.D.N., Cywinska A., Ball S.L., and deWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270(1512), 313–321.

https://doi.org/10.1098/rspb.2002.2218

Hebert P.D.N., Stoeckle M.Y., Zemlak T.S., and Francis C.M. (2004). Identification of birds through DNA barcodes. PLoS Biology, 2(10), e312. https://doi.org/10.1371/journal.pbio.0020312

Lebrun B., Romi-Lebrun R., Martin-Eauclaire M.F., Yasuda M., Ishiguro A., Oyama Y. and et al. (1997). A four-disulphide-bridged toxin, with high affinity towards voltage-gated K+ channels, isolated from Heterometrus spinnifer (Scorpionidae) venom. Biochemical Journal, 328(Pt 1), 321-327.

https://doi.org/10.1042/bj3280321

Lourenco W.R. and Pham D.S. (2010). A remarkable new cave scorpion of the family Pseudochactidae Gromov (Chelicerata, Scorpiones) from Vietnam. Zookeys, 71, 1-13. https://doi.org/10.3897/zookeys.71.786

Lourenco W.R. and Pham D.S. (2012). A second species of Vietbocap Lourenço and Pham, 2010 (Scorpiones: Pseudochactidae) from Vietnam. Comptes Rendus Biologies, 335(1), 80-85. https://doi.org/10.1016/j.crvi.2011.11.004

Lourenco W.R., Pham D.S. and Tran T.H. (2018). The genus Vietbocap Lourenco and Pham, 2010 in the Thien Duong cave, Vietnam: A possible case of subterranean speciation in scorpions (Scorpiones: Pseudochactidae). Comptes Rendus Biologies, 341, 264-273.

https://doi.org/10.1016/j.crvi.2018.03.002

Moritz C. and Cicero C. (2004). DNA barcoding: promise and pitfalls. PLoS Biol. 2004 Oct;2(10):e354. https://doi.org/10.1371/journal.pbio.0020354

Martin M.F. and Rochat H. (1984). Purification and amino acid sequence of toxin I" from the venom of the North African scorpion Androctonus australis Hector. Toxicon, 22(5), 695-703.

https://doi.org/10.1016/0041-0101(84)90153-3

Prendini L., Ehrenthal V.L. and Loria S.F. (2021). Systematics of the relictual Asian scorpion family Pseudochactidae Gromov, 1998 with a review of cavernicolous, troglobitic and troglomorphic scorpions. Bulletin of the American Museum of Natural History, 453(1), 1-149. https://doi.org/10.1206/0003-0090.453.1.1

Rajendra W., Armugam A. and Jeyaseelan K. (2004). Neuroprotection and peptide toxins. Brain Research Reviews, 45(2), 125-141. https://doi.org/10.1016/j.brainresrev.2004.04.001

Ratnasingham S. and Hebert P.D.N. (2007). Bold: The Barcode of Life Data System (http://www.barcodinglife.org). Molecular Ecology Notes, 7, 355-364.

https://doi.org/10.1111/j.1471-8286.2007.01678.x

Tamura K., Stecher G. and Kumar S. (2021). MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution,38 (7), 3022-3027. https://doi.org/10.1093/molbev/msab120

Zamudio F.Z., Conde R., Arevalo C., Becerril B., Martin B.M., Valdivia H.H. and et al. (1997). The mechanism of inhibition of ryanodine receptor channels by imperatoxin I, a heterodimeric protein from the scorpion Pandinus imperator. Journal of Biological Chemistry, 272(18), 11886-11894.

https://doi.org/10.1074/jbc.272.18.11886

Zhu S., Gao B., Aumelas A., del Carmen Rodriguez M., Lanz-Mendoza H., Peigneur S. and et al. (2010). MeuTXKbeta1, a scorpion venom-derived two-domain potassium channel toxin-like peptide with cytolytic activity. Biochimica et Biophysica Acta, 1804(4), 872-883. https://doi.org/10.1016/j.bbapap.2009.12.017