Micropropagation and analysis of somaclonal variation in “My Da” strawberry (<em> Fragaria ananassa </em> Duch.) using rapd marker

Ngoc Ai Trinh; Khac Nhu Nghi

doi:10.15625/2615-9023/22798

Author affiliations

Authors

Ngoc Ai Trinh Tra Vinh University
Nghi Khac Nhu Trường Đại học Trà Vinh

DOI:

https://doi.org/10.15625/2615-9023/22798

Keywords:

Coconut coir, meristem culture, multiplication, plant regulators, root formation, strawberry

Abstract

In vitro propagation of strawberry presents many challenges that must be addressed to enhance survival rates during ex vitro acclimation and to ensure the production of high-quality seedlings. This study aimed to develop an optimized protocol for the disinfection, multiplication, rooting, and acclimatization of “My Da” cultivar. Results showed that treating runner segments of “My Da” cultivar with 1% NaClO was effective. For multiplication, Murashige and Skoog (MS) medium supplemented with 0.5 mg. L^-1 BAP with 0.3 mg. L^-1 kinetin for “My Da” yielded the highest shoot proliferation, leaf count, and plant height. Rooting was most effective on half-strength MS medium supplemented with 0.3 mg. L^-1 BAP provided optimal rooting results. In the acclimatization stage, seedlings grown in organic compost was an improved survival rate. RAPD analysis revealed cultivar-dependent somaclonal variation, with the highest polymorphism in the OPK03 marker. The genotype-specific protocol supports efficient large-scale propagation and highlights the need for genetic monitoring in commercial systems.

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References

The findings achieved in the present study are an optimal protocol for micropropagation and ex vitro acclimatization of the “My Da” cultivar. Our results revealed that treatment with 1% NaClO for 20 min showed high efficiency in decreasing contamination and elevating meristem viability after the disinfection in “My Da” cultivar. The MS medium supplemented with 0.5 mg.L-1 BAP + 0.3 mg.L-1 Kinetin were the optimal media for the shoot proliferation and growth (the highest number of shoots and leaves, and greater plant height). The 1/2 MS medium supplemented with 0.1 mg.L-1 NAA + 0.1 mg.L-1 BAP was suitable for root formation. During ex vitro acclimatization, organic compost substrate was optimal for “My Da” strawberry. Moreover, using a molecular marker helps evaluate the genetic variation of meristem-derived and conventionally propagated strawberry plants was conducted in these strawberry cultivars.

Acknowledgements: We acknowledge the support of time and facilities from Tra Vinh University (TVU) for this study.

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