TY - JOUR AU - Hoai Sam, Vu AU - Thi Xuyen, Nguyen AU - Thi Huong, Nguyen AU - Thi Ngoc Anh, Duong AU - Minh Tuyen, Nguyen AU - Duy Phuong, Nguyen AU - Thuy Hien, Phan PY - 2020/11/28 Y2 - 2024/03/29 TI - Study on tuberization of Rehmannia glutinosa (Gaertn.) Libosch. JF - Vietnam Journal of Biotechnology JA - Vietnam J. Biotechnol. VL - 18 IS - 3 SE - Articles DO - 10.15625/1811-4989/18/3/15713 UR - https://vjs.ac.vn/index.php/vjbt/article/view/15713 SP - 507-516 AB - <em>Rehmannia glutinosa</em> (Gaertn.) Libosch. is an herbal plant, which belongs to the Srcophulariaceae family, containing the main active ingredients such as catalpol and verbacoside in its root tubers. In traditional medicine, <em>R. glutinosa</em> tubers have been used in the fresh or dried tuber root or the prepared rehamannia root. They not only possess the comprehensive pharmacological actions in the blood system, endocrine system but also used mainly for anti-tumor treament, immune-enhancement, anti-diabetes, treament for concretion in the urinary tract, etc. <em>R. glutinosa</em> is naturally distributed in China, Korea and Japan. This species was introduced into Viet Nam since 1958 and then planted widely in many the Northern plain and midland provinces. In Vietnam, <em>R. glutinosa</em> produces flowers without seeds, therefore the plant has been mainly propagated by slicing tubers. In this work, we investigated the effect of some plant hormones and sucrose contents on the ability to producing microtubers of <em>R. glutinosa</em>. Experiments were establishmented on the 1/4 MS medium supplemented with 50g/L sucrose.  Results showed that auxin (IBA, NAA) had no effect on the <em>in vitro</em> tuber formation of <em>R. glutinosa</em>. The efficiency of using the single BAP or PP<sub>333</sub> was low. The highest <em>in vitro</em> tuberization rate of <em>R. glutinosa</em> obtained on the medium supplemented with the combination of BAP (1.0 mg/L) and PP<sub>333</sub>  (0.3 mg/L), reached 83.33% and the number of tubers/plant was 5.58. The optimal sucrose concentration for increasing the diameter and weight of microtubers was 70 g/L. 100% of plants with tuber-roots survived in the nursery and thrived on the field ER -