TY - JOUR AU - Xuan Thach, Tran AU - Thi Thu, Ha AU - Thi Hien, Vu AU - The Hung, Hoang AU - Thi Hoa, Nguyen AU - Thu Hong, Le Thi AU - Ngoc Anh, Luu Dam AU - Van Huong, Bui AU - Lan Anh, La Thi AU - Van Quyen, Dong AU - Thi Tuyet Nhung, Nguyen PY - 2020/11/28 Y2 - 2024/03/28 TI - Linoleic acid isomerization ability of Lactobacillus spp. isolated from Vietnamese human intestinal origins JF - Vietnam Journal of Biotechnology JA - Vietnam J. Biotechnol. VL - 18 IS - 3 SE - Articles DO - 10.15625/1811-4989/18/3/13901 UR - https://vjs.ac.vn/index.php/vjbt/article/view/13901 SP - 445-453 AB - <p>Conjugated linoleic acid (CLA) have been shown to exert numerous health benefits, including anti-carcinogenic, anti-atherogenic, anti-diabetic, antiobesity, cholesterol reducing, antioxidant, anti-microbial, immune system modulator and growth-stimulating properties. In human, CLA is produced from Linoleic acid (LA) by gut bacteria. In this study, nineteen <em>Lactobacillus</em> (Lac.) strains isolated from human feces were studied to determine their ability to metabolize LA. The bacteria were grown in the liquid form of anaerobic MRS medium supplemented with 0.5 mg/mL LA. The linoleate isomerase activity in bacteria grown on MRS medium was determined by Gas chromatograpy. The results indicated that 4 out of 19 strains, including strains Lac.02, Lac.05, Lac.14 and Lac.16 are capable of producing about 40-50 μg/mL CLA from LA. Among them, the highest ability to produce CLA from LA is Lac.02 strain. In the production of CLA from LA, enzymes involved in this metabolism in <em>Lactobacillus</em> act as catalysts of hydration/dehydration (CLA-HY), oxidation of hydroxy groups/reduction of oxo groups (CLA-DH), migration of carbon-carbon double bonds (CLA-DC), and saturation of carbon-carbon double bonds (CLA-ER). The <em>cla</em>-<em>dh</em>, <em>cla</em>-<em>dc</em>, <em>cla</em>-<em>hy</em> and <em>cla</em>-<em>er</em> genes that encode enzymes CLA-DH, CLA-DC, and CLA-ER had been found in all Lac.02, Lac.05, Lac.14 and Lac.16 strains. Gas chromatography traces indicated that these strains produced the same compounds, which was subsequently identified as <em>cis</em>-9, <em>trans</em>-11, and <em>trans</em>-10, <em>cis</em>-12 CLA. In the next study, we will optimize the conditions such as substrate concentrations, pH values, temperature and culture time of each strain to obtain the best rerults.</p> ER -