@article{Thao_Nga_Cuc_Phuong_Ha Phuong_Hai Yen_Tuan Anh_2020, title={Promising activity against NTERA-2 cancer stem cells of malloapelta B compound isolated from the Bum-bup plant in Vietnam}, volume={18}, url={https://vjs.ac.vn/index.php/vjbt/article/view/15270}, DOI={10.15625/1811-4989/18/1/15270}, abstractNote={<span>Recent studies have revealed that cancer stem cells (CSCs) drive tumor growth, metastasis, therapy resistant and recurrence. Therefore, CSCs are considered as new targets for screening of more effective drugs. The compound </span><span lang="PT-BR">malloapelta B, which was isolated from the Bum-bup plant (<em>Mallotus apelta</em>) (Lour.) Muel.-Arg, Euphorbiaceae) in Vietnam, presented very potent and strong anticancer <em>in</em></span><em><span lang="PT-BR">vitro</span></em><span lang="PT-BR">, especially against the activ</span><span>a</span><span lang="PT-BR">tion of</span><span lang="PT-BR">NF-kB (nuclear factor- kappa B). In our research, malloapelta B inhibited the growth of NTERA-2 line</span><span>, a CSC line,</span><span lang="PT-BR"> at the value of IC<sub>50</sub> = 12.71 ± 0.76 µM. In this study, </span><span>m</span><span lang="PT-BR">alloapelta B also </span><span>had effects on cell cycle of NTERA-2 by significantly reducing the cell number at G0/G1 phase (37.48%), while raising this number of phase G2/M to 31.12% in comparison with those of control (56.81% and 18.6%, respectively). </span><span>Besides, clonogenic and tumorspheroidal formations are acknowledged as typically renewable characteristics of CSCs. Then, in our studies, malloapelta B significantly prevented NTERA-2 to form clonogens and reduced tumorspheres’ sizes at treatments with </span><span lang="NL">20 and 100 µg/mL. However, </span><span>malloapelta B did not exhibit any obvious effects on the expression level of </span><span lang="PT-BR">CD44<sup>+</sup>/CD24<sup>+</sup>, the two </span><span>typical cell surface markers of CSCs, at 2.5 and 5 </span><span lang="PT-BR">µM treatment</span><span>s</span><span lang="PT-BR">.</span>}, number={1}, journal={Vietnam Journal of Biotechnology}, author={Thao, Do Thi and Nga, Nguyen Thi and Cuc, Nguyen Thi and Phuong, Do Thi and Ha Phuong, Trieu and Hai Yen, Pham Thi and Tuan Anh, Hoang Le}, year={2020}, month={Jul.}, pages={117–125} }