Study of suitable media for laccase synthesis of basidiomycete fungi from Bidoup - Nuiba park and its reactive dye decolorization ability

Trần Thị Thu Hiền, Lê Thị Hiền, Nguyễn Văn Huynh, Đinh Thị Thu Hằng, Đặng Thị Cẩm Hà
Author affiliations

Authors

  • Trần Thị Thu Hiền Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
  • Lê Thị Hiền Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
  • Nguyễn Văn Huynh Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
  • Đinh Thị Thu Hằng Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
  • Đặng Thị Cẩm Hà Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam

DOI:

https://doi.org/10.15625/1811-4989/14/2/9357

Keywords:

Decolorization, inducer, laccase, mediator, Polyporus

Abstract

A fungal strain FBD154 with high laccase production was isolated from Bidoup-Nui Ba Parks, Lam Đong, Vietnam. FBD154 was identified as Polyporus sp. FBD154 based on traditional method and ITS sequence analysis. Polyporus sp. FBD154 synthesized laccase with high activity 74925 U/l on modified TSH1 (200 g/l containing potato extract, 1 g/l casein, 1 g/l rice bran, 5 g/l soybean meal, 10 g/l manose, 3 g/l NH4Cl, 0.3 mM CuSO4, pH 4). Crude laccase of Polyporus sp. FBD154 was applied to decolor several synthetic and commercial dyes. After 30 min, laccase from Polyporus sp. FBD154 could decolorize synthetic dyes at 100 mg/l concentration with efficiency of 60% acid red (NY1), 18% acid red 299 (NY7), 52% acid blue 281 (NY5) and 83% Remazol Brilliant Blue R (RBBR) without mediator. For commercial dyes at concentration of 100 mg/l, color removal efficiency reached to 62% megafix black CLS (CLS) and 72% everzol red LF2B (LF-2B) without mediator. Efficiency of synthetic dye removal by Polyporus sp. FBD154 crude laccase with 200 µM mediator obtained 90% RBBR with violuric acid (VIO) after 30 min; 80% NY5 with hydroxybenzotriazole (HBT) for 24 hours; 87% NY1 with acetosyringone (Ace) for 5 min; 92% NY7, 91% LF-2Band 73% CLS with syringaldehyde (Syr) for 5 min. The obtained evidences show that laccase was synthesized by Polyporus sp. FBD154 with high potential for application in wastewater treatment of textile plants in particular as well a sin detoxification of polycyclic aromatic compounds in general.

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Published

30-06-2016

How to Cite

Hiền, T. T. T., Hiền, L. T., Huynh, N. V., Hằng, Đinh T. T., & Hà, Đặng T. C. (2016). Study of suitable media for laccase synthesis of basidiomycete fungi from Bidoup - Nuiba park and its reactive dye decolorization ability. Vietnam Journal of Biotechnology, 14(2), 313–325. https://doi.org/10.15625/1811-4989/14/2/9357

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Articles