Resveratrol inhibits cytokine production in LPS-stimulated RAW264.7 cells potentially through TLR4/MyD88/NF-κB pathway

To Minh Nhat, Tran Thu Trang, Nguyen Trung Nam
Author affiliations

Authors

  • To Minh Nhat University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam https://orcid.org/0009-0004-9390-8444
  • Tran Thu Trang Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Trung Nam Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/20426

Abstract

Resveratrol is a naturally occurring compound with anti-inflammatory properties. However, the protective molecular mechanisms of resveratrol against LPS-induced inflammation have not been thoroughly known. In the present study, we examined the anti-inflammatory effect of resveratrol in inflammatory model using murine macrophage-like cell RAW264.7 stimulated with LPS. Resveratrol suppressed the production of inflammatory cytokines in LPS-stimulated RAW264.7 cells with the IC50 value as 17.5 ± 0.7 μM for IL-6, 14.2 ± 1.9 μM for IL-10, and 18.9 ± 0.6 μM for TNF-α. Gene expression of TLR4, MyD88 and NF-κB were significantly suppressed by resveratrol treatment in LPS-stimulated RAW264.7 cells. In conclusion, the anti-inflammatory property of resveratrol is potentially related to its inhibitory effecton TLR4/MyD88/NF-κB signaling pathway in macrophages.

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Published

30-12-2023

How to Cite

Nhat, T. M., Thu Trang, T., & Nam, N. T. (2023). Resveratrol inhibits cytokine production in LPS-stimulated RAW264.7 cells potentially through TLR4/MyD88/NF-κB pathway. Vietnam Journal of Biotechnology, 21(4), 611–619. https://doi.org/10.15625/1811-4989/20426

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