Study on the transient expression of infectious bronchitis virus spike protein in Nicotiana benthamiana leaves

Nguyen Thi Tra, Ngo Hong Duong, Trinh Thai Vy, Le Thi Tra My, Nguyen Thi Thu Hien, Pham Thi Van, Doan Thi Thanh Huong, Pham Bich Ngoc, Chu Hoang Ha, Hoang Thi Thu Hang
Author affiliations

Authors

  • Nguyen Thi Tra Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam https://orcid.org/0009-0001-7068-2006
  • Ngo Hong Duong Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam https://orcid.org/0009-0002-4523-3919
  • Trinh Thai Vy Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Le Thi Tra My Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Nguyen Thi Thu Hien Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Pham Thi Van Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Doan Thi Thanh Huong Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Pham Bich Ngoc Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Chu Hoang Ha Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Hoang Thi Thu Hang Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/19591

Abstract

Infectious bronchitis virus (IBV) is considered as one of the main causes of economic loss in chicken farms worldwide, especially in poultry producing nations. This virus, a member of the Coronaviridae family, is classified into different genotypes based on its surface spike glycoproteins which also play important roles in cell attachment and immune response. Due to continual genetic mutation of IBV, attenuated and inactivated vaccines show decreased effectiveness or lack of cross – protection; thus ongoing studies focus on the development of new generation vaccines to prevent new IBV outbreaks. This paper describes our study on expression of the antigenic region in S1 subunit of the spike in Nicotiana benthamiana. Two expression vectors carrying either S1 or receptor binding domain (RBD) coding gene (pCB301-S1 and pCB301-RBD) were constructed and transformed into tobacco leaves by agroinfiltration method. The RBD showed a clearly higher level of expression compared to the whole S1. Purification of RBD by immobilized metal ion chromatography and size exclusion chromatography represented a mixture of monomer, dimer and trimer glycoprotein with expected sizes in Western blot. In summary, this study demonstrated our primary success in establishing an expression model that could be used to investigate plant-based IBV recombinant vaccine.

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Published

30-09-2023

How to Cite

Tra, N. T., Hong Duong, N., Thai Vy, T., Tra My, L. T., Thu Hien, N. T., Van, P. T., Thanh Huong, D. T., Bich Ngoc, P., Hoang Ha, C., & Thu Hang, H. T. (2023). Study on the transient expression of infectious bronchitis virus spike protein in Nicotiana benthamiana leaves. Vietnam Journal of Biotechnology, 21(3), 531–541. https://doi.org/10.15625/1811-4989/19591

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