Optimization of fermentation medium for spore production of Paenibacillus polymyxa IN937a and its antifungal activity

Authors

  • Pham Trung Hieu Center for High Technology Development, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Tran Dai Lam Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Le The Tam Vinh University, 182 Le Duan Road, Vinh City, Nghean Province, Vietnam
  • Vu Thi Thoa Research and Development Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry (VIIC), 2 Pham Ngu Lao, Hoan Kiem District, Ha Noi, Vietnam
  • Nguyen Thi Phuong Thao Institute of Tropical Biology, Vietnam Academy of Science and Technology 9/621 Hanoi Highway, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam
  • Ho Tu Cuong Institute of Environmental Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam
  • Vu Dinh Hoang Hanoi University of Science and Technology,1 Dai Co Viet Road, Hai Ba Trung District, Hanoi, Vietnam
  • Le Dang Quang Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/17202

Abstract

In this study, we optimized the fermentation medium for spore production of Paenibacillus polymyxa IN937a. Seven factors including molasses, glucose, magnesium sulfate, potassium pyrophosphate, yeast extract, zinc sulfate, and ammonium sulfate are selected as the basis for the screening of factors affecting the spore production of P. polymyxa IN937a by the Plackett-Burman experiment. Based on the analysis of the Plackett-Burman matrix, the result showed that yeast extract, molasses, and ammonium sulfate were the three main impact factors (P < 0.05), which affected the yield of P. polymyxa IN937a spores. Then, the optimum combination of the three factors was subsequently optimized by the response surface methodology (RSM) using Box-Behnken design to increase the spore production in P. polymyxa IN937a fermentation. The obtained results by RSM predicted that maximum spore density of P. polymyxa IN937a was 6.606×109 spore/mL after 48 hours of the experiment when the appropriate medium for the spore production of P. polymyxa IN937a included yeast extract 14.44 g/L, molasses 19.14 g/L, and ammonium sulfate 0.20 g/L. In addition, the antifungal activity of P. polymyxa IN937a was also tested in this study. The preliminary results of in vitro antifungal activity indicated that P. polymyxa IN937a had a good inhibition on the growth of two phytopathogenic fungal strains Sclerotium rolfsii and Rhizoctonia solani. These results could be used for further research on the fermentation of P. polymyxa IN937a on a pilot scale to obtain the optimal number of spores for use in the development of biological crop protection products.

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Published

2022-03-30

How to Cite

Trung Hieu, P., Dai Lam, T., The Tam, L., Thi Thoa, V., Thi Phuong Thao, N., Tu Cuong, H., Dinh Hoang, V., & Dang Quang, L. (2022). Optimization of fermentation medium for spore production of Paenibacillus polymyxa IN937a and its antifungal activity. Vietnam Journal of Biotechnology, 20(1), 117–124. https://doi.org/10.15625/1811-4989/17202

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