Optimization of Citrus limon peel extraction, determination of main chemical components and effectiveness in repellency against Aedes mosquito dengue fever vector

Authors

  • Phung Thi Kim Hue Institute of Health Research and Educational Development in Central Highlands, 73 Le Hong Phong Street, Pleiku City, Gia Lai Province, Vietnam
  • Tran Van Loc Institute of Health Research and Educational Development in Central Highlands, 73 Le Hong Phong Street, Pleiku City, Gia Lai Province, Vietnam
  • Le Tri Vien Institute of Health Research and Educational Development in Central Highlands, 73 Le Hong Phong Street, Pleiku City, Gia Lai Province, Vietnam
  • Le Dung Sy Institute of Health Research and Educational Development in Central Highlands, 73 Le Hong Phong Street, Pleiku City, Gia Lai Province, Vietnam
  • Ho Viet Hieu Institute of Health Research and Educational Development in Central Highlands, 73 Le Hong Phong Street, Pleiku City, Gia Lai Province, Vietnam
  • Le Thi Nhung Hung Vuong Gifted High School, 48 Hung Vuong Street, Pleiku City, Gia Lai Province, Vietnam
  • Le Thanh Do Duy Tan University, 254 Nguyen Van Linh, Thanh Khe District, Da Nang City, Vietnam
  • Pham Thi Khoa Institute of Health Research and Educational Development in Central Highlands, 73 Le Hong Phong Street, Pleiku City, Gia Lai Province, Vietnam

DOI:

https://doi.org/10.15625/1811-4989/16365

Abstract

Currently, scientific publications are focusing on the pharmacological actions of Citrus limon's extract and essential oil. Its essential oil is rich in bioactive monoterpenoids, such as D-limonene, β-pinene, γ-terpinene. Citrus limon essential oils have been recognized as the potential source of natural insecticides because of their selectivity, ability to be biologically degraded into non-toxic compounds, low impacts on biodiversity and the environment. Many previous studies have reported anti-bacterial, anti-fungal, anti-inflammatory, anti-cancer, hepato-regenerative, and cardio-protective activities of Citrus limon essential oil. In this study, we collected the discarded Citrus limon peel source in Gia Lai province to utilize as a material to build an optimized extraction process with the following criteria: extraction solvent, solvent/sample ratio, extraction temperature, and extraction time. The results showed a stable extraction process with a maximum extraction efficiency of 4.02%, at 40°C, for 3 hours, with two extraction times using 95% ethanol for solvent. Using GC/MS method, the determined limonene content accounted for 12.2% of the extract. The Citrus limon peel extract exhibited potency against Aedes aegypti (arbovirus vector) at a concentration of 0.01 mL, with protection time of 70 minutes and biting percentage of 0.9%, compared to negative control with statistically significant (P < 0.05). The above results correspond with the most recent publications about the effects of mosquito repellence of certain plant-based essential oils. This study has proven that Citrus limon peel in this locality signifies a promising candidature for future studies regarding its main active compound, limonene, in the control of dengue-transmitting vectors. Therefore, Citrus limon peel extract brings hope to develop new mosquito repellency products in the future.

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Published

2022-06-30

How to Cite

Hue, P. T. K., Van Loc, T., Tri Vien, L., Dung Sy, L., Viet Hieu, H., Nhung, L. T., Thanh Do, L., & Khoa, P. T. (2022). Optimization of Citrus limon peel extraction, determination of main chemical components and effectiveness in repellency against Aedes mosquito dengue fever vector. Vietnam Journal of Biotechnology, 20(2), 329–338. https://doi.org/10.15625/1811-4989/16365

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Articles