Effect of silver nanoparticles on sterilization of different explant sources of Gerbera jamesonii cultured in vitro

Vu Thi Hien; Hoang Thanh Tung; Hoang Dac Khai; Vu Quoc Luan; Do Manh Cuong; Tran Van Lich; Bui Van The Vinh; Trinh Thi Huong; Duong Tan Nhut

doi:10.15625/1811-4989/15672

Author affiliations

Authors

Vu Thi Hien
Hoang Thanh Tung Viện Nghiên cứu Khoa học Tây Nguyên https://orcid.org/0000-0002-2614-0429
Hoang Dac Khai
Vu Quoc Luan
Do Manh Cuong
Tran Van Lich
Bui Van The Vinh
Trinh Thi Huong
Duong Tan Nhut

DOI:

https://doi.org/10.15625/1811-4989/15672

Keywords:

Cây hoa đồng tiền, nano bạc, khử trùng mẫu cấy, vi nhân giống

Abstract

In this study, silver nanoparticles effects on the sterilization of different sources of explants (young leaves, young flower stalks and young flower buds) of Gerbera as well as on the in vitro morphogenesis and their growth were investigated. The explants were sterilized and cut transversally (1 mm) with the flower stalk, square (0.5 × 0.5 cm) for the leaf sample, longitudinally (0.5 mm) for the flowers (removed the petals) and cultured on MS medium; then, the explants (contamination-free or no browning/necrosis) were transferred into MS medium supplemented with 0.02 mg/L TDZ plus 0.8 mg/L adenine, 10% coconut water, 30 g/L sucrose and 8 g/L agar in 15 days. The results showed that AgNPs at the appropriate concentration and duration treatment was effective in explant sterilization of flower bud (0.02% AgNPs and 20 min), flower stalks (0.02% AgNPs and 30 min) and young leaves (0.05% AgNPs and 20 min) after 15 days of culture. In addition, 3 types of morphogenesis including callus induction, somatic embryogenesis and direct shoot regeneration of explants derived from sterilization by AgNPs were improved as compared to that of HgCl₂. In addition, research on the optimal medium for shoot multiplication, rooting as well as evaluation of acclimatization and the growth at the greenhouse were also studied. Results showed that MS medium supplemented with 2 mg/L NAA combined with 0.5 mg/L BA and 2 mg/L KIN is suitable for shoot multiplication; meanwhile, MS medium supplemented with 2 mg/L NAA improved rooting ability as well as quality of plantlets and to improving survival rate and acclimatization of Gerbera cultured in vitro.

Downloads

Download data is not yet available.

References

Amoo SO, Van Staden J (2013) Influence of plant growth regulators on shoot proliferation and secondary metabolite production in micropropagated Huernia hystrix. Plant Cell Tiss Org Cult 112(2): 249-256.

Aswath C, Choudhary ML (2002) Rapid plant regeneration from Gerbera jamesonii Bolus callus cultures. Acta Bot Croatica 61: 125-134.

Bais HP, Sudha G, Suresh B, Ravishankar GA (2000) AgNO3 influences in vitro root formation in Decalepisha miltonii Wight and Arn. Curre Sci 79: 894-898.

Cardoso JC, da Silva JAT (2012) Micropropagation of Gerbera using chlorine dioxide (ClO2) to sterilize the culture medium. In Vitro Cell Dev Bio - Plant 48(3): 362-368.

Chau NH, Bang L, Buu N, Dung T, Ha H, Quang D (2008) Some results in manufacturing of nanosilver and investigation of its application for disinfection. Adv Nat Appl Sci 9(2): 241-248.

Constantine DR (1986) Micropropagation in the commercial environment. In: Withers L, Alderson PG (eds). Plant Tissue Culture and Its Agricultural Applications. London, Butterworth, 175-186.

Đỗ Mạnh Cường, Lê Thành Long, Hoàng Thanh Tùng, Vũ Quốc Luận, Vũ Thị Hiền, Nguyễn Thị Nhật Linh, Trương Thị Bích Phượng, Dương Tấn Nhựt (2018) Vai trò của nano bạc trong khử trùng, cảm ứng mẫu cấy ban đầu và nâng cao tần suất hình thành tế bào đơn cây hoa salem (Limonium sinuatum (L.) Mill). Tạp chí Công nghệ Sinh học 16(3): 481-490.

Đỗ Mạnh Cường, Trương Thị Bích Phượng, Dương Tấn Nhựt (2018) Ảnh hưởng của nano bạc lên khả năng cảm ứng mô sẹo và tái sinh chồi từ mẫu cấy lá cây dâu tây (Fragaria x ananassa) nuôi cấy in vitro. Tạp chí Khoa học Đại học Huế 127(1C): 61-70.

Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11(1): 1-42.

Dương Tấn Nhựt, Dương Bảo Trinh, Đỗ Mạnh Cường, Hoàng Thanh Tùng, Nguyễn Phúc Huy, Vũ Thị Hiền, Vũ Quốc Luận, Lê Thị Thu Hiền, Nguyễn Hoài Châu (2018) Khảo sát nano bạc làm chất khử trùng mẫu mới trong nhân giống vô tính cây African violet (Saintpaulia ionantha H. Wendl.). Tạp chí Công nghệ Sinh học, 16(1): 87-97.

Hà Thị Mỹ Ngân, Trần Đào Hồng Trinh, Đỗ Mạnh Cường, Hoàng Thanh Tùng, Nguyễn Thị Nhật Linh, Vũ Thị Hiền, Phan Lê Hà Nguyễn, Vũ Quốc Luận, Bùi Văn Lệ, Dương Tấn Nhựt (2019) Hạn chế hiện tượng thủy tinh thể và gia tăng tỷ lệ sống của cây con hoa đồng tiền (Gerbera jamesonii) nuôi cấy in vitro trong môi trường bổ sung nano bạc. Tạp chí Công nghệ Sinh học 17(1): 115-124.

Ines M, Krunoslav D, Vesna T, Marija V, Ankica P, Zlatko C, Boris P, Zorica J (2013) In vitro sterilization procedures for micropropagation of Oblaciska sour cherry. J Agric Sci 58(2): 117-126.

Khan N, Ahmed M, Hafiz I, Abbasi N, Ejaz S, Anjum M (2015) Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes. Oeno One 49(1): 37-45.

Masoud F, Abdolreza B, Ahmad S (2012) Disinfecting effects of nano silver fluids in Gerbera (Gerbera jamesonii) capitulum tissue culture. J Biol Environ 6(17): 121-127.

Murashige T, Serpa M, Jones TB (1974) Clonal multiplication of Gerbera jamesonii through tissue culture. Hort Sci 9: 175-180.

Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Plant Physiol 1(3): 473-497.

Nabeel KA (2011) Using silver nano-particles to increase efficiency of sterile solution for in vitro techniques. Iraqi J Canc Med Genet 4(1): 48-51.

Ngan HTM, Cuong DM, Tung HT, Nghiep ND, Le BV, Nhut DT (2020) The efect of cobalt and silver nanoparticles on overcoming leaf abscission and enhanced growth of rose (Rosa hybrida L. ‘Baby Love’) plantlets cultured in vitro. Plant Cell Tiss Org Cult 141: 393-405.

Nhut DT, An TTT, Huong NTD, Don NT, Hai NT, Thien NQ, Vu NH (2007) Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture. Sci Hortic 111(2): 146-151.

Orlikowska T, Nowak E, Marasek A, Kucharska D (1999) Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from gerbera petioles. Plant Cell Tiss Org Cult 59(2): 95-102.

Pilon-Smits EAH, Quinn CF, Tapken W, Malagoli M, Schiavon M (2009) Physiological functions of beneficial elements. Curr Opin Plant Biol 12: 267-274.

Rafsanjani MSO, Alvari A, Samim M, Hejazi MA, Abdin MZ (2012) Application of novel nanotechnology strategies in plant biotransformation: A contemporary Overview. Recent Pat Biotech 6:69-79.

Shabanpour KAA, Sharifi A, Bagheri A, Moshtaghi N (2011) Effect of genotypes and culture medium on shoot regeneration and proliferation of Gerbera jamesonii. Afr J Biotech 10(57): 12211-12217.

Shah V, Belozerova I. (2008), Influence of metal nanoparticles on the soil microbial community and germination of lettuce seeds. Water Air Soil Poll 197: 143-148.

Shokri S, Babaei A, Ahmadian M, Hessami S, Arab MM (2014) The effects of different concentrations of nano silver on elimination of bacterial contaminations and phenolic exudation of Rose (Rosa hybrida L.) in vitro culture. Inter J Farm Allied Sci 3(1): 50-54.

Trần Hiếu, Hoàng Thanh Tùng, Cao Đăng Nguyên, Dương Tấn Nhựt (2018) Tạo nguồn mẫu in vitro cho giống chanh dây tím (Passiflora edulis Sims.) và vàng (Passiflora edulis f. flavicarpa). Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên 127(1C): 71-84.