TY - JOUR AU - Quang, Le Trong AU - Nam, Nguyen Phuong AU - Ha, Phan Thi AU - Tran Chau, Pham Thi PY - 2015/09/02 Y2 - 2024/03/28 TI - Purification and some properties of two protein proteinase inhibitors from the seeds of Sophora japonica L. f. JF - Academia Journal of Biology JA - AJB VL - 25 IS - 3 SE - Articles DO - 10.15625/0866-7160/v25n3.6839 UR - https://vjs.ac.vn/index.php/vjbio/article/view/6839 SP - 59-65 AB - <p>Using column chromatography on superdex-75, MonoS and PAGE methods allowed to separate two highly purified protein proteinase inhibitors from <em>Sophora japonica</em> seeds, named SJ-I and SJ-II. Each of them showed inhibitory activity against both trypsin and chymotripsin.</p> <p>The molecular mass of both inhibitors were estimated around 16 kDa. However, they were different in pI value: 6.2 for SJ-I and 5.85 for SJ-II.</p> <p>The heat stability of two inhibitors was also similar: after the treatment of inhibitors at 70<sup>o</sup>C for 3 min, TIA remained 40% and after 15 min, TIA completely lost.</p> <p>All obtained data led to a suggestion that SJ-I and SJ-II were isoforms and might be a Bowman - Birk type double headed inhibitors of higher molecular mass.</p> ER -