TY - JOUR AU - Nga, Vuong Thi AU - Hoa, Kieu Quynh AU - Man, Tran Dinh AU - Hien, Lai Thuy PY - 2015/03/19 Y2 - 2024/03/29 TI - Medium optimization for biosurfactant production by Rhodococcus ruber TD2 using response surface methodology JF - Academia Journal of Biology JA - AJB VL - 36 IS - 3 SE - Articles DO - 10.15625/0866-7160/v36n3.5976 UR - https://vjs.ac.vn/index.php/vjbio/article/view/5976 SP - 360-366 AB - With the advanced properties such as excellent emulsifying activity, reducing surface tension, antitumor and immunomodulation, <em>Rhodococcus</em> biosurfactants have attracted a great deal of interest from biologists community. Development of optimized procedures for their biosynthesis would increased application potentials of <em>Rhodococcus</em> biosurfactants in new advanced technologies, such as environmental bioremediation, biomedicine, etc. The goal of this study is to determine the medium optimization for biosurfactant production by <em>Rhodococcus</em> <em>ruber</em> TD2 using  response surface methodology (RSM) based on changes of medium component: diesel oil (DO), NaNO<sub>3</sub> and pH. All of three factors were the most significant influences on its biosurfactant biosynthesis. RSM analysis showed that the highest biosurfactant production was achieved with medium containing 5.7% (v/v) DO, 3.3 gL<sup>-1</sup> NaNO<sub>3</sub> and pH 8.3. In this condition, strain TD2 produced maximum yield of crude biosurfactant, which was 30.1 gL<sup>-1 </sup>after 5 days. This value was 2.23- fold higher than before medium optimization. The data showed that RSM was highly efficient in optimizing biosurfactant  production by <em>Rhodococcus ruber</em> TD2 isolated from coastal zone of Vietnam. ER -