TY - JOUR AU - Thach, Bui Dinh AU - Long, Le Thanh AU - Quynh Chi, Ho Nguyen AU - Duy, Nguyen Khac AU - Chung, Doan Chinh AU - Si, Do Minh AU - Ho, Nguyen Huu AU - Suong, Ngo Ke AU - Son, Hoang Nghia PY - 2014/10/28 Y2 - 2024/03/29 TI - Plumbagin induces apoptosis characteristics in HepG2 cells JF - Academia Journal of Biology JA - AJB VL - 36 IS - 2 SE - Articles DO - 10.15625/0866-7160/v36n2.5123 UR - https://vjs.ac.vn/index.php/vjbio/article/view/5123 SP - 247-252 AB - This study is to investigate the relationship between anti-proliferative activity of plumbagin and apoptosis induction of plumbagin on HepG2 cancer cells. The results showed that plumbagin treated HepG2 cell density was decreased. The apoptotic morphologies were also observed in HepG2 cells after plumbagin treatment. Nucleus staining, RT-PCR and comet assay were applied to study apoptotic morphologies in HepG2 cells. Acridine Orange staining results demonstrated that fragmented HepG2 nucleus by plumbagin was one of the important proves for itsĀ  apoptotic induced characteristics. <em>Bax</em> transcript expression was increased in plumbagin treated HepG2 cells. However, there was no difference of bcl-2 transcripts expression between plumbagin treated HepG2 and control, suggesting that plumbagin induced a change in <em>bax</em> and <em>bcl-2</em> transcripts expression of HepG2. Comet assay was applied to measure genomic DNA fragmentation in HepG2 cells. Fragmented DNA percentage in comet tail and comet tail length were increased in HepG2 treating with higher plumbagin concentrations. In conclusion, plumbagin showed apoptotic induced activitiy through morphological changesĀ  in HepG2 cells. ER -