TY - JOUR AU - Thao, Nguyen Thi AU - Khoa, Dao Trong AU - Giang, Le Quang AU - Huyen, Do Thi AU - Trung, Nguyen Thi AU - Thu Hien, Nguyen Thi AU - Huy, Nguyen Quoc AU - Hai, Truong Nam PY - 2014/08/23 Y2 - 2024/03/29 TI - Identification of termite Coptotermes species collected from Linh Quang pagoda, Ha Dong, Hanoi based on DNA sequences of the fragments in the mitochondrial 16s rRNA gene JF - Academia Journal of Biology JA - AJB VL - 36 IS - 1 SE - Articles DO - 10.15625/0866-7160/v36n1.4518 UR - https://vjs.ac.vn/index.php/vjbio/article/view/4518 SP - 58-64 AB - <p>In 2004, Szalanski et al. developed PCR method based on 2 pairs of specific primers to amplify 2 DNA fragments of 428 bps and 151 bps located in the mitochondrial 16S rRNA for identifying <em>C. formosanus</em> from other <em>Coptotermes</em> species. According to the study, a <em>Coptotermes</em> species is <em>C. formosanus</em> if PCR products were composed of two DNA fragments of 428 bps and 151 bps observed in agarose gel. In this study, using the primers, 2 DNA fragments of 248 bps and 160 bps were amplified from genome of <em>Coptotermes </em>HN1 collected from Ling Quang pagoda. In agarose gel, these DNA fragments looked the same sizes with the DNA amplified from genome of <em>C. formosanus</em> by Szalanski et al. (2004). However, multialigment showed that the sequences were most homologous with the sequences of <em>C. gestroi</em>. Phylogenetic trees were built, based on 40 homologous genes (for each DNA fragment) in GenBank. <em>Coptotermes</em> HN1 belonged to <br /> <em>C. gestroi</em> branch, and was far to the branch of <em>C. formosanus</em> in the phylogenetic tree. So the <em>Coptotermes</em> HN1 collected from Linh Quang pagoda, Ha Dong, Hanoi was designated as <em>C. gestroi</em> HN1. From this study, the identification of <em>C. formosanus</em> by PCR developed by Szalanski et al. should have more consideration.</p> ER -