TY - JOUR AU - Thu Huyen, Nguyen Thi AU - Yen, Dang Thi AU - Yen, Nguyen Thi AU - Nga, Vuong Thi AU - Hien, Lai Thuy PY - 2013/01/13 Y2 - 2024/03/29 TI - Using of response surface methodology for optimization of biohydrogen production by Clostridium sp. tr2 isolated in Vietnam JF - Academia Journal of Biology JA - AJB VL - 34 IS - 4 SE - Articles DO - 10.15625/0866-7160/v34n4.2686 UR - https://vjs.ac.vn/index.php/vjbio/article/view/2686 SP - 479-484 AB - Biohydrogen is a clean, renewable, sustainable energy resource due to the highest energy density among all fuels and its combustion has no contribution to the environmental pollution and climate change. Biohydrogen production depends on a number of nutritional and environmental variables. The present paper is to determine the optimum condition for enhanced hydrogen production by a fermentative hydrogen-producing bacterium (designated as <em>Clostridium</em> sp. Tr2) isolated from buffalo-dung in Vietnam. The response surface methodology (RSM) was employed to determine the mutual effects of glucose, yeast extract and iron concentration on its hydrogen production in a batch condition. RSM analysis showed that the highest hydrogen production potential (Ps) was obtained under the condition of 10.18 g L<sup>-1 </sup>glucose, 2.5 g L<sup>-1</sup> yeast extract and 58 mg L<sup>-1 </sup>FeSO<sub>4</sub>.7H<sub>2</sub>O. All three factors had significant influences on the Ps. Glucose and iron concentration, yeast extract and iron concentration were interdependent or there was a significant interaction on Ps. Glucose and yeast extract concentration was slightly interdependent, or their interactive effect on Ps was not significant. Under optimum conditions, the maximum H<sub>2</sub> volume of 1080 ml (L medium)<sup>-1</sup> were found after 22 h facultative anaerobic fermentation. The experiment results show that the RSM analysis with the central composite design was useful for optimizing the biohydrogen-producing process by newly isolated <em>Clostridium </em>sp. Tr2 in Vietnam. ER -