TY - JOUR AU - Khoa, Tran Tu AU - Duy, Pham Minh AU - Huong, Tran Thi AU - Quynh, Nguyen Thi PY - 2021/03/31 Y2 - 2024/03/29 TI - Effects of in vitro plant ages on the subsequent growth of Plumbago indica l. after ex vitro transplantation JF - Academia Journal of Biology JA - AJB VL - 43 IS - 1 SE - Articles DO - 10.15625/2615-9023/15798 UR - https://vjs.ac.vn/index.php/vjbio/article/view/15798 SP - AB - <p>The Indian leadwort (<em>Plumbago indica</em> L.) of the family Plumbaginaceae is a plant with high pharmaceutical value, as it contains plumbagin, a naphthoquinone with antibacterial, antifungal and anticancer properties. Among the propagation methods for the Indian leadwort, <em>in vitro</em> propagation is considered an effective method in producing disease-free transplants in a short period of time with high propagation rate. When plants grown <em>in vitro</em> are transferred to <em>ex vitro</em> condition, the environmental factors in the nursery house such as light, temperature, humidity and microorganism in the soil will affect their growth. Characteristics of transplants themselves is also critical for the subsequent growth. It is, thus, essential to establish the standards to evaluate and qualify <em>in vitro</em> plants for transplanting to <em>ex vitro</em> condition. Among these standards, the culture age of <em>in vitro</em> plants affects the maturations of their root, stem and leaves, which can in turn influence the acclimating ability and growth of <em>in vitro</em> plants after transplantation. The purpose of this study is to investigate the effects of the culture age of <em>in vitro</em> Indian leadwort plants on their performance during <em>ex vitro</em> stage. For this purpose, three different culture ages of uniform <em>in vitro</em> plants, 35, 42 and 49 day-old, were studied. After 28 days of cultivation in the nursery house under the light intensity of 70 ± 10 µmol m<sup>-2</sup> s<sup>-1</sup>, temperature of 35 ± 4 <sup>o</sup>C and relative humidity (RH) of 60 ± 10%, all three treatments achieved 100% survival rate. Increased fresh and dry weights and percentage of dry matter after cultivation in <em>ex vitro</em> condition were not statistically different between 42 day-old and 49 day-old <em>in vitro</em> plants, but were significantly different between these plants and 35 day-old <em>in vitro</em> plants. The development of shoot and root in <em>ex vitro</em> stage of 42 day-old and 49 day-old <em>in vitro</em> plants was more balanced, as shown by the higher ratio of shoot/root dry weight, than 35 day-old <em>in vitro</em> plants. The results of this study showed that for this <em>Plumbago</em> species, bigger <em>in vitro</em> plants led to better growth during <em>ex vitro</em> stage. These results also indicated that it was possible to transfer <em>in vitro</em> <em>Plumbago</em> plants to <em>ex vitro</em> condition after 5 weeks of <em>in vitro</em> culture stage.</p><p><em> </em><em></em></p><div><p> </p></div> ER -