TY - JOUR AU - Phuong Duyen, Nguyen Thuy AU - Van, Tran Thi AU - Le Minh, Nguyen Thu AU - Quynh, Nguyen Thi PY - 2018/02/28 Y2 - 2024/03/29 TI - Effects of micro-environmental factors on the photoautotrophic growth of Hibiscus sagittifolius Kurz cultured in vitro JF - Academia Journal of Biology JA - AJB VL - 39 IS - 4 SE - Articles DO - 10.15625/0866-7160/v39n4.11030 UR - https://vjs.ac.vn/index.php/vjbio/article/view/11030 SP - 496-506 AB - <p>The arrow leaf abelmoschus rhizome (<em>Hibiscus sagittifolius </em>Kurz), or Sam Bo Chinhin Vietnamese, is a perennial suffrutex herb from which the tuber root is used as a medicine in folk remedy. This species is widely distributed and can be found on many terrains across South East Asia. With an aim to create a large number of uniform and high-quality <br /> <em>H.</em><em> sagittifolius</em><em> </em>transplants <em>in vitro</em>, effects of some environmental factors such as photoperiod and temperature on the photoautotrophic growth of <em>H.</em><em> sagittifolius</em><em>in vitro</em> plants were investigated. <em>In vitro</em> single nodal cuttings, each with an unfolded leaf, were cultured photoautotrophically (without sucrose and vitamins) on mineral SH medium, under light intensity of 150 µmol m<sup>-2</sup> s<sup>-1</sup>, temperature of 24<sup>o</sup>C ± 2<sup>o</sup>C, relative humidity (RH) of 55% ± 5% and three different photoperiods (8, 12 or 16 h d<sup>-1</sup>) in the first experiment. Commercial polypropylene bags (V = 1,000 ml), attached with two filter-paper membranes, were used as culture vessels. After 42 days of culture, <br /> <em>H.</em><em> sagittifolius</em><em> </em>plants under the photoperiod of 16 h d<sup>-1</sup> had the greatestincreased fresh weight (502.3 mg/plt), increased dry weight (39.5 mg/plt) and leaf area (17.0 cm<sup>2</sup>) than those put under 8 hd<sup>-1</sup>or 12 h d<sup>-1</sup>. In addition, <em>H.</em><em> sagittifolius</em><em> </em>plants also showed statistical differences in growth when photoautotrophically cultured in different air temperature regimes, including 28/25<sup>o</sup>C (photo-/dark period), 25/25<sup>o</sup>C and 20/25<sup>o</sup>C. Increased fresh weight (775 mg/plt), increased dry weight (62 mg/plt) and leaf area (22.7 cm<sup>2</sup>) of <em>in vitro</em><em> </em><em>H.</em><em> sagittifolius</em><em> </em>plants were the greatest when temperature was maintained at 28 <sup>o</sup>C during photoperiod. On the contrary, the photoperiod temperature of 20<sup>o</sup>C resulted in the shortest shoot length and root length of <em>H.</em><em> sagittifolius</em>plants. In conclusion, this study proved that <em>H</em><em>.</em><em> sagittifolius</em> plants had the best growth when cultured on SH medium, without sucrose and vitamins supplementation, under light intensity of 150 µmol m<sup>-2</sup> s<sup>-1</sup>, RH 55% ± 5%, photoperiod of 16 h d<sup>-1</sup>, temperature regime of 28/25<sup>o</sup>C(photo-/dark period).</p><p> </p><p><em>Citation</em>: Nguyen Thuy Phuong Duyen, Tran Thi Van, Nguyen Le Thu Minh, Nguyen Thi Quynh, 2017. Effects of micro-environmental factors on the photoautotrophic growth of <em>Hibiscus sagittifolius</em> Kurz cultured <em>in vitro</em>. Tap chi Sinh hoc, 39(4): 496-506. DOI: <a href="http://dx.doi.org/10.15625/0866-7160/v36n4.6177">10.15625/0866-7160</a>/v39n4.11030. </p><p>*<em>Corresponding author</em>: qtnguyen_vn@yahoo.com</p><p><em>Received 7</em><em> September 2017</em><em>, accepted 15 December 2017</em></p> ER -