@article{Hong Trang_Tuong_Thanh Van_Tung_Son_Mau_2016, title={The characteristics of gmchi gene isolated from soybean cultivars with different isoflavone content}, volume={38}, url={https://vjs.ac.vn/index.php/vjbio/article/view/7959}, DOI={10.15625/0866-7160/v38n2.7959}, abstractNote={<p>In this study, isoflavone content of five soybean varieties disseminated grown in North Vietnam have been surveyed, and the <em>GmCHI</em> gene encoding chalcone isomerase, a key enzyme in metabolizing synthetic isoflavone in soybeans, was analyzed. Content of isoflavone (daidzein, genistein) in 3-day-old germinated seeds of soybean cultivar DT26 was the highest (64.27 mg/100g) and higher 2.18 times and 2.45 times compared with DT84 and DT2008 respectively. <em>GmCHI</em> gene isolated from mRNA of the soybean cultivars DT26, DT51, DT2008, DT84 is 657 nucleotides in length, encoding 218 amino acids. Similarity coefficient of nucleotide sequence of <em>GmCHI</em> gene of four soybean DT26, DT51, DT2008, DT84 ranged from 96.8% to 98.9% and similar to those of NM_001248290 on GenBank from 98% to 99%. Genetic distances of DT26 compared with DT51 DT26, DT2008, DT84 and other varieties based on nucleotide sequences of <em>GmCHI</em> gene was 19.3% and based on the amino acid sequence deduced was 4.4%. Coding region of <em>GmCHI</em> gene has been used as a material for design transfomation vector for the improvement of isoflavone content in soybean by gene transfer technique.</p><p> </p>}, number={2}, journal={Academia Journal of Biology}, author={Hong Trang, Le Thi and Tuong, Ho Manh and Thanh Van, Tran Thi and Tung, Pham Thanh and Son, Le Van and Mau, Chu Hoang}, year={2016}, month={Jun.}, pages={236–242} }