@article{Uyen_Thuan_Thanh Loi_Hong Hanh_2015, title={Detection of the Thermolabile hemolysin (tlh) and thermostable drect hemolysin (tdh) gene of Vibrio parahaemolyticus by PCR}, volume={25}, url={https://vjs.ac.vn/index.php/vjbio/article/view/6856}, DOI={10.15625/0866-7160/v25n4.6856}, abstractNote={<p><em>Vibrio parahaemolyticus</em> is a halophytic bacterium found in black tiger shrimp culture ponds and is associated with shrimp diseases. All <em>V. parahaemolyticus </em>strains carry the thermolablile hemolysin (<em>tlh</em>) that appears to be species specificgene. The production of the thermostabile direct hemolysin (<em>tdh</em>) by <em>V. parahaemolyticus</em> is associated pathogenically qith the organism and is encoded by the <em>tdh</em> gene. The <em>V. parahaemolyticus</em> biochemical indentication method requires 5-8 days and did not detect the pathogen strains. The PCR and multiplex PCR methods which amplify <em>tlh</em> and <em>tdh</em> genes are used to detect various strains of <em>V. parahaemolyticus</em>. All 7 isolated <em>V. parahaemolyticus</em> strains are controlledby the API 20E showed and by PCR method, show the presemee of the <em>tlh</em> gene, however only two strains VpPM4 and ATCC17803 carry the <em>tdh</em> gene. The multiplex PCR method is also and successfully to detect these two strains VpPM4 and ATCC17803.</p>}, number={4}, journal={Academia Journal of Biology}, author={Uyen, Nguyen Hoang and Thuan, Nguyen Chi and Thanh Loi, Nguyen Thi and Hong Hanh, Nguyen Thi}, year={2015}, month={Sep.}, pages={42–46} }