@article{Duy_Quynh_2014, title={Growth and lignan accumulation of Phyllanthus amarus (Schum. & Thonn.) cultured in vitro photoautotrophically as affected by light intensity and photoperiod}, volume={36}, url={https://vjs.ac.vn/index.php/vjbio/article/view/5117}, DOI={10.15625/0866-7160/v36n2.5117}, abstractNote={<p>Light intensity and photoperiod are two important factors to the growth and secondary metabolite accumulation of <em>in vitro</em> plants, especially under photoautotrophic micropropagation. Internodal segments carrying 1 leaf of <em>in vitro</em> <em>Phyllanthus amarus</em> plants were used as explants. Four explants were put on MS medium with half strength macro elements and without sugar and vitamins, in a Magenta box-type vessel (V = 370 ml). There were two holes (Ф = 1 cm) covered by Millipore membranes (Ф = 0.45 µm) on the vessel lid. Perlite was used as supporting material. The experiment was carried out with 3 different light intensities (80, 120 or 160 µmol m<sup>-2</sup> s<sup>-1</sup>) in combination with 3 levels of photoperiod (8, 12 or 16 hours per day), under room temperature of 25 ± 2<sup>o</sup>C and relative humidity of 50%. On day 40, <em>Phyllanthus amarus</em> plants grew better under higher PPF and longer photoperiod. Increased fresh weight and dry weight, stem diameter and shoot lenght of <em>in vitro</em> plants were the highest under the PPF of 160 μmol m<sup>-2 </sup>s<sup>-1</sup> and the photoperiod of 16 hours per day. Phyllanthin, hypophyllanthin and niranthin contents were the largest when plants were cultured under the PPF of 80 µmol m<sup>-2 </sup>s<sup>-1</sup> and the photoperiod of 12 hours per day.</p>}, number={2}, journal={Academia Journal of Biology}, author={Duy, Pham Minh and Quynh, Nguyen Thi}, year={2014}, month={Oct.}, pages={203–209} }