@article{Hien_Thom_Ha_Anh_Hong_2020, title={Optimization of culture conditions for squalene production and squalene extraction method of <i>Thraustochytrium<\i> sp. TN22}, volume={42}, url={https://vjs.ac.vn/index.php/vjbio/article/view/14978}, DOI={10.15625/2615-9023/v42n4.14978}, abstractNote={&lt;p&gt;Squalene, a natural triterpene with important roles as antioxidant, skin hydrating, cardio-protection and detoxifier, has attracted the attention of researchers in the world. This study investigated the optimal culture conditions for squalene production and to develop a method for squalene extraction and purification from cell suspension of &lt;em&gt;Thraustochytrium&lt;/em&gt; sp. TN22. The results showed that squalene production by the strain &lt;em&gt;Thraustochytrium &lt;/em&gt;sp. TN22 was optimum at 2% glucose, 0.5% yeast extract and 0,14% mixture of vitamins (B&lt;sub&gt;1&lt;/sub&gt;, B&lt;sub&gt;6&lt;/sub&gt; and B&lt;sub&gt;12&lt;/sub&gt;) at 28 &lt;sup&gt;o&lt;/sup&gt;C for 2 days of cultivation. At the optimum conditions, the dry biomass, squalene content and productivity were approximately 9.3 g/L, 9.9 mg/g DCW and 95.3 mg/L, respectively. Time-dependence on cell lysis and extraction solvents were selected as the extraction parameters. The obtained results showed that the highest squalene production of 178.1 mg/100 g biomass was obtained in cell lysate at alkaline medium (pH = 10), at 60 &lt;sup&gt;o&lt;/sup&gt;C for 6 hours and n-hexan was the best solvent for squalene extraction. Squalene was then purified on silica gel column using n-hexan as the mobile phase and 90% purity of squalene was archived. Our obtained results are promising for the commercial productions of various value-added compounds from species belong to the genus &lt;em&gt;Thrautochytrium &lt;/em&gt;in Vietnam.&lt;/p&gt;&lt;p&gt; &lt;/p&gt;}, number={4}, journal={Academia Journal of Biology}, author={Hien, Hoang Thi Minh and Thom, Le Thi and Ha, Nguyen Cam and Anh, Hoang Thi Lan and Hong, Dang Diem}, year={2020}, month={Oct.} }